Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Elucidation of Specific RNAs by Treatment of Rhus verniciflua Stokes Extract to U937 Cell

옻추출물 처리에 의한 U937 세포에서의 특정 RNA 발현 양상

  • Jeong, Mi-Young (School of Life Sciences and Biotechnology, Korea University) ;
  • Oh, Se-Wook (Food Safety Research Division, Korea Food Research Institute)
  • 정미영 (고려대학교 생명공학원) ;
  • 오세욱 (한국식품연구원 안전성연구단)
  • Published : 2008.10.31
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Abstract

Differential display RT-PCR was used for screening the differentially expressed specific genes by Rhus verniciflua extract treatment to U937 cell, human leukemic monocyte. As a result, 19 clones differentially expressed were detected. Among the detected clones, one clone was confirmed to be over-expressed by R. verniciflua extract treatment in Northern blot analysis. Nucleotide sequence of the clone showed 100% homology with H2A histone family member Z gene. Therefore, it is concluded that the treatment of R. verniciflua extract to U937 cell specifically induces the expression of H2A.Z gene but its role should be elucidated by future works.

사람의 혈액 내의 단핵구 U937을 모델시스템으로 이용하여 옻추출물 처리에 의해 발현이 조절되는 특정 유전자를 탐색하였다. DDRT-PCR을 이용하여 옻 추출물 처리 시 발현이 감소되는 클론을 하나 얻을 수 있었으며 이를 클로닝하고 염기서열 분석을 실시하였다. 그 결과 얻어진 유전자는 H2A histone family의 member Z와 100% 유사성이 있는 것으로 나타났다. 이 단백질은 특정 조건 하에서 특정한 유전자 발현을 증가시키는 역할을 하는 것으로 보고되고 있으나, 보다 정확한 작용기작을 알아내기 위해서는 유전자 관계 파악을 위하여 향후 계속적인 연구가 필요함을 알 수 있었다.

Keywords

References

  1. Shin MK. Coloured Kimsangbonchobak. Namsandang, Seoul, Korea. pp. 165-718 (1986)
  2. Jung NC. Biological activity of urushiol and flavonoids from lac tree (Rhus verniciflua Strokes). PhD thesis, Chonnam National University, Gwangju, Korea (1998)
  3. Lee SK, Kang SM, Kim YS, Kang CG. Quality composition of emulsion-type sausages made from Rhus verniciflua stokes fed pork and extract. Korean J. Food Sci. Anim. Resour. 25: 210-217 (2005)
  4. Buckingham J. Dictionary of Natural Products. Chapman and Hal I. London, England p. 761 (1994)
  5. Lee JC, Lim KT, Jang YS. Identification of Rhus verniciflua Strokes compounds that exhibit free radical scavenging and antiapototic properties. Biochim. Bioph. Acta 1570: 181-191 (2002) https://doi.org/10.1016/S0304-4165(02)00196-4
  6. Lim KT, Shim JH. Antioxidative effects of ethanol extracts from Rhus verniciflua Stokes (RVS) on mouse whole brain cells. Korean J. Food Sci. Technol. 29: 1248-1254 (1997)
  7. Kim IW, Shin DH, Baek NI. Identification of antioxidative components from ethanol extract of Rhus verniciflua STOKES. Korean J. Food Sci. Technol. 31: 1654-1660 (1999)
  8. Kim JB. Identification of antioxidative component from stem bark of Rhus verniciflua. Korean J. Food Nutr. 16: 60-65 (2003)
  9. Lee JC, Lee KY, Kim J, Na CS, Jung NC, Chung GH, Jang YS. Extract from Rhus verniciflua Stokes is capable of inhibiting the growth of human lymphoma cells. Food Chem. Toxicol. 42: 1383-1388 (2004) https://doi.org/10.1016/j.fct.2004.03.012
  10. Lee JC, Kim J, YS Jang. Ethanol-eluted extract of Rhus verniciflua Stokes inhibits cell growth and induces apoptosis in human lymphoma cells. J. Biochem. Mol. Biol. 36: 337-343 (2003) https://doi.org/10.5483/BMBRep.2003.36.4.337
  11. Liang CY, Kang SM, Kim YS, Lee SK. Effect of dietary Rhus vernificlua Stokes on the quality of hanwoo (Korean cattle) beef during cold storage after thawing. Korean J. Food Sci. Anim. Resour. 25: 196-202 (2005)
  12. Kim KM, Kim TY, Kim MK, Kim HR. Quality properties of Gangjung added with detoxified stem bark of Rhus verniciflua (RVSB) extract during acceleration storage. Korean J. Food Sci. Technol. 39: 425-431 (2007)
  13. Christoph FA, Vogel Eric Sciullo, Fumio Matsumura. Activation of inflammatory mediators and potential role of Ah-receptor ligands in foam cell formation. Cardiovasc. Toxicol. 4: 363-373 (2004) https://doi.org/10.1385/CT:4:4:363
  14. Paul E. DiCorleto, Carol A. de la Motte. Characterization of the adhesion of the Human Monocytic cell line U937 to cultured endothelial cells. J. Clin. Invest. 75: 1153-1161 (1985) https://doi.org/10.1172/JCI111810
  15. Roy L. Silverstein, Adam S. Asch, Ralph L. Nachman. Glycoprotein IV mediates thrombospondin-dependent platelet-monocyte and platelet-U937 cell adhesion. J. Clin. Invest. 84: 546-552 (1989) https://doi.org/10.1172/JCI114197
  16. Johan F, Jan N, Anders H, Anders H, Hans W, Magnus G. Oxidized low density lipoprotein induces differentiation and adhesion of human monocytes and the monocytic cell line U937. P. Natl. Acad. Sci. USA 87: 904-908 (1990)
  17. Okubo T, Yokoyama Y, Kano K, Kano I. Molecular mechanism of cell death induced by the antioxidant tert-butylhydroxyanisole in human monocytic leukemia U937 cells. Biol. Pharm. Bull. 27: 295-302 (2004) https://doi.org/10.1248/bpb.27.295
  18. Yokoyama Y, Okubo T, Ozawa S, Nagai F, Ushiyama K, Kano I,Shioda M, Kubo H, Takemura M, Namiki H, Yasugi E, Oshima M, Seyama Y, Kano K. CPP32 activation during dolichyl phosphate- induced apoptosis in U937 leukemia cells. FEBS Lett. 412: 153-156 (1997) https://doi.org/10.1016/S0014-5793(97)00763-1
  19. Troyano A, Fernandez C, Sancho P, de Blas E, Aller P. Effect of glutathione depletion on antitumor drug toxicity (apoptosis and necrosis) in U-937 human promonocytic cells. The role of intracellular oxidation. J. Biol. Chem. 276: 47107-47115 (2001) https://doi.org/10.1074/jbc.M104516200
  20. Zhao S, Ooi, SL, Pardee AB. New primer strategy improves precision of differential display. Biotechniques 18: 842-850 (1995)
  21. Namrita D, Masaya O, Shawn JS, Oscar MA, Rohinton TK. $ H_2A$.Z funtions to regulate progression through the cell cycle. Mol. Cell. Biol. 26: 489-501 (2006) https://doi.org/10.1128/MCB.26.2.489-501.2006
  22. Maryse A, Francois R, Marc L, Luc G. H2A.Z is required for global chromatin integrity and for recruitment of RNA polymerase II under specific conditions. Mol. Cell. Biol. 21: 6270- 6279 (2001) https://doi.org/10.1128/MCB.21.18.6270-6279.2001
  23. Marc L, Luc G. $ H_2A$.Z has a function reminiscent of and activator required for preferential binding to intergenic DNA. EMBO J. 22: 4512-4522 (2003) https://doi.org/10.1093/emboj/cdg427