Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Ascorbic Acid, Silicon, Fe, Proline and Lysine on Proliferation and Collagen Synthesis in the Human Dermal Fibroblast Cell (HS27)

비타민 C, Silicon, 철분, Proline 및 Lysine의 처리가 피부 섬유아세포의 증식 및 Collagen I과 III의 발현에 미치는 효과 비교

  • Kim, Sun-Ah (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Jin-Ah (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Jung-Min (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Hyun-Ae (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Young-Ae (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Yun, Hye-Jeong (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Cho, Yun-Hi (Dept. of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 김선아 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이진아 (경희대학교 동서의학대학원 의학영양학과) ;
  • 김정민 (경희대학교 동서의학대학원 의학영양학과) ;
  • 김현애 (경희대학교 동서의학대학원 의학영양학과) ;
  • 김영애 (경희대학교 동서의학대학원 의학영양학과) ;
  • 윤혜정 (경희대학교 동서의학대학원 의학영양학과) ;
  • 조윤희 (경희대학교 동서의학대학원 의학영양학과)
  • Published : 2009.11.30
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Abstract

In the dermis, fibroblast plays an important role in the turnover of the dermal extracellular matrix. Collagen I and III, which are the most important dermal proteins of the extracellular matrix, function as a stabilizing scaffold of dermal connective tissues, as well as a regulator of differentiation and migration of dermal cells. In this study, we investigated the effect of various nutrients, such as ascorbic acid, silicon, Fe, lysine and proline which function as cofactors or building blocks on collagen synthesis. When the physiological concentrations of ascorbic acid (0-100 ${\mu}M$), silicon (0-50 ${\mu}M$), Fe (0-50 ${\mu}M$), lysine (0-150 ${\mu}M$) and proline (0-300 ${\mu}M$) were treated at HS27 for either 3 or 5 days, 5 day treatment of ascorbic acid at the low concentration (5-10 ${\mu}M$) increased the expression of collagen I and III protein by 115-1300% without increasing cell proliferation. 3 or 5 days treatment of Fe increased the expression of collagen I and III proteins up to 323% in parallel with cell proliferation by 164%. However, cell proliferation and expression of collagen I and III protein in silicon treated HS27 did not differ. Proline and lysine only increased cell proliferation up to 247.9%. Taken together, we demonstrate that the physiological concentrations of ascorbic acid and Fe enhance the expression of collagen I and III protein for treatment of 3 or 5 days.

본 연구에서는 HS27 피부 섬유아세포에 비타민 C, silicon, 철분, proline 및 lysine을 혈청 및 피부 내 농도 범위로 처리한 후 세포의 증식을 비롯하여 콜라겐 I과 III의 mRNA 및 단백질의 발현을 비교하였다. 비타민 C는 처리 기간 및 농도와 무관하게 세포 증식 및 mRNA 발현에 영향을 미치지 않았으며 콜라겐 type I 및 III의 단백질 발현은 각각 5-50 ${\mu}M$, 10-50 ${\mu}M$ 농도에서 5일 처리 시 683.9%, 182% 이상으로 발현을 증가시켰다. Silicon의 3일 및 5일 농도별 처리는 세포증식과 콜라겐 mRNA 및 단백질 발현을 다소 감소시키거나 영향을 미치지 않았다. 철분의 3일 및 5일 처리는 혈청 내 농도(11-35 ${\mu}M$)에서 콜라겐 mRNA 발현에 영향을 미치지 않았으며 세포 증식 및 콜라겐 단백질 발현을 증가시켰다. 또한 철분의 5-30 ${\mu}M$의 농도 처리는 5일 처리보다 3일 처리 시 발현수준이 훨씬 컸다. 콜라겐의 구성 아미노산인 proline 및 lysine은 섬유아세포의 증식을 증가시켰다. 따라서 단기간의 콜라겐의 합성 증가는 철분의 섭취가 적합하나 장기간의 섭취에는 비타민 C의 섭취와 더불어 철분의 섭취가 필요한 것으로 여겨진다.

Keywords

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