대한약침학회지 (Journal of Pharmacopuncture)

  • 제12권4호
  • /
  • Pages.63-76
  • /
  • 2009
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
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膈下逐瘀湯이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 肝癌 形成과 肝損傷에 미치는 영향

The Effect of the Keughachukeo-tang Extract on the Hepatocellular Carcinogenesis and Acute Liver Damage Induced by Diethylnitrosamine and CCl4 in Rats

  • 허래경 (원광대학교 한의과대학 내과학교실) ;
  • 성기문 (원광대학교 한의과대학 내과학교실) ;
  • 김소연 (원광대학교 한의과대학 내과학교실) ;
  • 제준태 (원광대학교 한의과대학 내과학교실) ;
  • 권소연 (원광대학교 한의과대학 내과학교실) ;
  • 문구 (원광대학교 한의과대학 내과학교실) ;
  • 이종덕 (원광대학교 광주병원 영상의학과) ;
  • 원진희 (원광대학교 한의과대학 내과학교실)
  • Heo, Rae-Kyong (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Seung, Kee-Moon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Kim, So-Yeon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Je, Jun-tae (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Kwon, So-yeon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Moon, Goo (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Lee, Jong-Deok (Department of Radiology, Won-kwang University Hospital) ;
  • Won, Jin-Hee (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University)
  • 발행 : 2009.12.30
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초록

This study was performed to observe the effect of Keughachukeo-tang(KH) extract on the hepatocellular carcinogenesis and acute liver damage induced by Diethylnitrosamine(DENA) and $CCl_4$ in Rats. Experimental groups were divided into four; normal group(Nor), acute liver damage and hepatocellular cancer inducing control group(Con), KH extract 350㎎/㎏/day(KHA), and 700㎎/㎏/day(KHB) administered groups to Con. The results obtained are as follows: The body weight increased in KHA and KHB than Con from 7th week to the 8th week. The activities of Alanine aminotransferase(ALT) were the most increased in the Con among experimental group. The activities of aspartate aminotransferase(AST), alkaline phosphatase(ALP), and lactacte dehydrogenase(LDH) were significantly decreased(p$<$0.05) in the KHA and KHB compared with Con. Alpha fetoprotein(AFP) were the most increased in the Con among experimental groups. The activities of superoxide dismutase(SOD) were the most increased in the Con among experimental groups. The activities of catalase were significantly increased(p$<$0.05) in the KHA and KHB compared with Con. The results of light microscopical observation, a number of hepatocytes were damaged in the Con compared with Nor and KH extract administerd groups. The number of hepatic p53 positive cells was reduced in the KH extract administered groups compared with Con. These results suggest that administration of KH extract suppress or retard on the Hepatocellular Carcinogenesis and acute liver damage induced by DENA and $CCl_4$ in Rats.

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참고문헌

  1. Devita VT, Hellman S, Rosenberg SA, Cancer. Principles and Practice of Oncology. Philadelphia. Lippincott Williams & Wilkins. 2004;7:17
  2. 의학교육연구원 편. 가정의학. 서울대학교 출판부. 1995:266.
  3. 통계청 편집부. 사망원인통계연보 전국편(`2006). 2007.
  4. No HJ, Jeon BH, Moon G, Moon SJ. Experimental Syudies on the Antitumor Effects of Sojeokbojungwhan Extract. J of Korean Oriental Oncology. 1996;2(1):43-56
  5. Jeong HW, Jeon BH, Jeong WY. Experimental Study of NaetakKanghwal-Tang against tumor and immunologic control function. Korean J of oriental medical pathology. 1997;11(2):81-91
  6. Yu DH, Eun JS, Kwon J, Oh CH. Effects of Sa-Mul-Tang on Immunocytes of L1210 Celltransplanted or Antitumor Drugs-administered Mice. Korean J of Pharmacognosy. 1998;29(2):110-119.
  7. Baek DG. Anticancer Effect of Paljinhangahm dan on Human Acute Myeloid Leukemia. J of Wonkwang Uni. 2002.
  8. Baek EG. Anticancer Effect of Combination with Paljinhangaham-dan and adriamycin on HepG2 Human Malignant Hepatoma Cell Line. J of Wonkwang Uni. 2003.
  9. 王淸任. 醫林改錯. 서울. 一中社. 1992:66-8.
  10. 余桂淸. 歷代中醫腫類案論選粹. 北京. 北京 出版社. 1988:1-49.
  11. Lee KH. A Study on the Effect of Keughachukeo-tang on Thrombosis and elevated Blood Viscosity. J of Kyunghee Uni. 1992:15-22.
  12. Reitman S, Frankel AS. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1987;28:56-63
  13. Lee HS, Chung YH, Kim CY. Specificities of serum alpha-fetoprotein in HBsAg+ and HBsAg- patients in the diagnosis of hepatocellular carcinoma. Hepatology. 1991;14:68-72 https://doi.org/10.1002/hep.1840140112
  14. Sato Y, Nakata K, Kato Y, et al. Early recognition of hepatocellular carcinoma based on altered profiles of alpha-fetoprotein. N Engl J Med. 1993;328:1802-1806. https://doi.org/10.1056/NEJM199306243282502
  15. Beers RF Jr, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1992;195(1):133-140.
  16. 이문호. 내과학. 서울. 금강출판사. 1970:2458-82.
  17. 서정선. 20세기말의 의 생물학의 새로운 비전 apoptosisa.몸의 효율적 생존을 위한 세포의 능동적 사망기전. 서울. 의학연구의 최신동향. 1998: 30-32.
  18. 王氷. 黃帝內經. 서울. 高文社. 1977:素問 91, 166, 229, 326 靈樞 76, 88.
  19. 上海中醫學院. 實用中醫內科學. 上海. 上海科學技術出版社. 1986: 621-35.
  20. 신민교. 臨床本草學. 서울. 남산당. 2000:172-5, 236-9, 242-4, 385-7, 466-8, 477-8, 483-4, 530-2, 534-5, 540-2, 728-9, 731-2.
  21. 李時珍. 本草綱目. 서울. 高文社. 1983:401, 467, 485, 488, 497, 517, 564, 996, 1116, 1189, 1466.
  22. 許浚. 東醫寶鑑. 서울. 南山堂. 1986:731.
  23. Kim HS, Kang JH, Kim CH, Bae GB, Kim JS, Park SJ, Yoon HJ, Kim SY, Gwac SH. The Effect of Nutritional Support with Nucare^R in Cancer Patients Receiving Chemotherapy. Chungnam medical J. 1997;24(2):181-189.
  24. 이귀녕, 권오현. 임상병리파일. 서울. 의학문화사. 2003:334-7, 345-6, 373-6, 399-404, 872-6.
  25. Takeda, Y, A Ichihara, H Taruoka and H Inove. The biochemistry of animal cells the effect of corticosteroids on leakage of enzyme from dispersed rat liver cell. J Biol Chem. 1964;239:3590-3596
  26. Brucker, JV, MF Mackenzie and S muralidhara. Oral toxicity of carbon tetrachloride Acute, subacute, and subchronic studies in rats. Fund Appl Toxicol. 1986:616-34.
  27. Dinman, BD, EA Hamdin, CF Fox, WJ Frajola WJ. CCl4 Toxicity III. Hepatostructural and enzyme change Arch Environ Health. 1963;7:630-46. https://doi.org/10.1080/00039896.1963.10663595
  28. 대한임상병리학회, 임상병리학, 고려의학, 1996:67-76.
  29. Henry RJ, Cannon DC, Winkelman JW. Clinical chemistry principles and techniques. New York. Harper and Row. 1974;2:829.
  30. Lee JH, Song IS, Kim YT, Chung YH, Lee HS, Kim CY, Jung HC, Yun YB. Relationship Between the Size of Hetatocellular Carcinoma and Serum Alpha-Fetoprotein Level and Ultrasonographic Echogenicity. Korean J Gastroenterol. 1988;20(3):679-684
  31. Fieg D.I., Reid T.M., and L.A. Reactive oxygen species in tumorigenesis. Cancer Res. 1994:1890-1894.
  32. Diguiseppi. J, and Fridovich I. Oxygen toxicity in streptococcus sangius. J. biol. Chem. 1982;257:4046-4051.
  33. Bellisola G, Casaril M, Gabrielli GB, Caraffi M, Corrocher R. Catalase activity in human hepatocellular carcinoma(HCC). Italy. Clin Biochem. 1987;20(6):415-417. https://doi.org/10.1016/0009-9120(87)90007-5
  34. Strobel P, K limea F, Zerban H, K opp-Schneider A, BaNKasch P. Xenomorphic hepatocellular precusor and neoplastic progression of tigroid cell foci induced in rats with low doses of N-nitrosomorpholine. Carcinogenesis. 1998;19:2069-2080. https://doi.org/10.1093/carcin/19.12.2069
  35. Steinberg P, Klingelhoffer A, Schafer A, Wust G, Weibe G, Oesch F, Eigenbrodt. Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats. Virchows Arch. 1999; 434:213-220. https://doi.org/10.1007/s004280050330
  36. Yu E, Lee I, Lee MJ, Jang JJ. Progressive changes of the nuclear matrix protein composition in diethylnitrosamine induced rat hepatocarcinogenesis. Cancer Lett. 1999;138:151-157. https://doi.org/10.1016/S0304-3835(98)00390-5
  37. Pruschy M, Rocha S. Key targets for the execution of radiation induced tumor cell apoptosis : the role of p53 and caspases. Int. J. Radiat. Oncol. Biol. Phys. 2001;49(2):561-567. https://doi.org/10.1016/S0360-3016(00)01480-2
  38. Jessup JM, Loda M. Prognostic markers in rectal carcinoma. Semin Surg Oncol.1998;15:131-140. https://doi.org/10.1002/(SICI)1098-2388(199809)15:2<131::AID-SSU11>3.0.CO;2-5
  39. Sturm I, Papadopoulos S, Hillebrand T, Benter T, Luck HJ, Wolff G, Dorken B, Daniel PT. Impaired BAX proteins expression in breast cancer:mutational analysis of the Bax and the p53 gene. Int J Cancer. 2000;87:517-521. https://doi.org/10.1002/1097-0215(20000815)87:4<517::AID-IJC9>3.0.CO;2-B