Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Free Radical Scavenging Activity and Protective Ability of Methanolic Extract from Duchesnea indica Against Protein Oxidation and DNA Damage

  • Hu, Weicheng (School of Biotechnology, Kangwon National University) ;
  • Shen, Wei (College of Guangling, Yangzhou University) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidant potency of methanolic extract of Duchesnea indica (MDI; Indian strawberry) was investigated by employing various established in vitro systems, such as total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay, metal chelating assay, superoxide radical scavenging activity and protective ability of DNA damage and protein oxidation. MDI inhibited metal chelating by 75.57% at 2 mg/mL, scavenged 50% DPPH free radical at 29.13 ${\mu}$g/mL, and eliminated approximately 46.21% superoxide radical at the concentration of 1 mg/mL. In addition, MDI showed strong ability on reducing power, DNA damage protection and protein oxidation protection. Overall, results suggested that MDI might be beneficial as a potent antioxidant and effectively employed as an ingredient in food applications.

Keywords

References

  1. Bandyopadhyay U, Das D, Banerjee RK. 1999. Reactive oxygen species: oxidative damage and pathogenesis. Cur Sci 77: 658-666
  2. Clarkson PM, Thompson HS. 2000. Antioxidants: what role do they play in physical activity and health. Am J Clin Nutr 72: 637-646
  3. Wang G, Alamuri P, Maier RJ. 2006. The diverse antioxidant systems of Helicobacter pylori. Mol Microbiol 61: 847-860 https://doi.org/10.1111/j.1365-2958.2006.05302.x
  4. Shukla S, Mehta A, John J, Singh S, Mehta P, Vyas SP. 2009. Antioxidant activity and total phenolic content of ethanolic extract of Caesalpinia bonducella seeds. Food Chem Toxicol 47: 1848-1851 https://doi.org/10.1016/j.fct.2009.04.040
  5. Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. 2002. Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical and hypochlorous acid. J Agric Food Chem 50: 4989-4993 https://doi.org/10.1021/jf020225o
  6. Cai Y, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 74: 2157-2184 https://doi.org/10.1016/j.lfs.2003.09.047
  7. Samarth RM, Panwar M, Kumar M, Soni A, Kumar M, Kumar A. 2008. Evaluation of antioxidant and radical- scavenging activities of certain radioprotective plant extracts. Food Chem 106: 868-873 https://doi.org/10.1016/j.foodchem.2007.05.005
  8. Lee IR, Kim YH. 1986. Studies on the antitumor activity of Duchesnea Indicae herba. Arch Pharm Res 9: 1-4 https://doi.org/10.1007/BF02857697
  9. Kim IG, Jung IL, Oh TJ, Kim KC, Shim HW. 2002. Polysaccharide-enriched fraction isolated from Duchesnea chrysantha protects against oxidative damage. Biotech Lett 24: 1299-1305 https://doi.org/10.1023/A:1019812202099
  10. Kim KC, Kim JS, Son JK, Kim IG. 2007. Enhanced induction of mitochondrial damage and apoptosis in human leukemia HL-60 cells by the Ganoderma lucidum and Duchesnea chrysantha extracts. Cancer Lett 246: 210-217 https://doi.org/10.1016/j.canlet.2006.02.014
  11. Lee IR, Yang MY. 1994. Phenolic compounds from Duchesnea chrysantha and their cytotoxic activities in human cancer cell. Arch Pharm Res 17: 476-479 https://doi.org/10.1007/BF02979129
  12. Qiao W, Bao Z, Zhang W, Duan HQ. 2009. Two new triterpens from Duchesnea indica. Chin Chem Lett 20: 572-575 https://doi.org/10.1016/j.cclet.2008.12.052
  13. Peng JN, Lu YR, Chen DH. 1995. Studies on the chemical constituents of Indian mock strawberry. Chin Tradit Herb Drugs 26: 338-341
  14. Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. 2009. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tisssues. Pak J Pharm Sci 22: 277-281
  15. Hu W, Heo SI, Wang MH. 2009. Antioxidant and anti-inflammatory activity of Kalopanax pictus leaf. J Korean Soc Appl Biol Chem 52: 360-366 https://doi.org/10.3839/jksabc.2009.064
  16. Que F, Mao L, Zhu C, Xie G. 2006. Antioxidant properties of Chinese yellow wine, its concentrate and volatiles. LWT-Food Sci Technol 39: 111-117 https://doi.org/10.1016/j.lwt.2005.01.001
  17. Singh N, Rajini PS. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85: 611-616 https://doi.org/10.1016/j.foodchem.2003.07.003
  18. Qian ZJ, Jung WK, Byun HG, Kim SK. 2008. Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Bioresour Technol 99: 3365-3371 https://doi.org/10.1016/j.biortech.2007.08.018
  19. Kızıl G, Kızıl M, Ceken B. 2009. Protective ability of ethanol extracts of Hypericum scabroides Robson & Poulter and Hypericum tiquetrifolium Turra against protein oxidation and DNA damage. Food Sci Biotechnol 18: 130-136
  20. Jung CH, Seong HM, Choi IW, Park MW, Cho HY. 2006. Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Sci Technol 39: 266-274 https://doi.org/10.1016/j.lwt.2005.01.004
  21. Shoemaker M, Hamilton B, Dairkee SH, Cohen I, Campbell MJ. 2005. In vitro anticancer activity of twelve Chinese medicinal herbs. Phytother Res 19: 649-651 https://doi.org/10.1002/ptr.1702
  22. Wang J, Yuan X, Jin Z, Tian Y, Song H. 2007. Free radical and reactive oxygen species scavenging activities of peanut skins extract. Food Chem 104: 242-250 https://doi.org/10.1016/j.foodchem.2006.11.035
  23. Ferrari CKB, Torres EAFS. 2003. Biochemical pharmacology of functional foods and prevention of chronic diseases of aging. Biomed Pharmacother 57: 251-260 https://doi.org/10.1016/S0753-3322(03)00032-5
  24. Siriwardhana N, Lee KW, Jeon YJ, Kim SH, Haw JW. 2003. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Technol Int 9: 339-346 https://doi.org/10.1177/1082013203039014
  25. Milardovi S, Ivekovi D, Grabari BS. 2006. A novel amperometric method for antioxidant activity determination using DPPH free radical. Bioelectrochemistry 68: 175-180 https://doi.org/10.1016/j.bioelechem.2005.06.005
  26. Pin-Der-Duh X. 1998. Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free-radical and active oxygen. J Am Oil Chem Soc 75: 455-461 https://doi.org/10.1007/s11746-998-0248-8
  27. Tanaka M, Kuie CW, Nagashima Y, Taguchi T. 1988. Applications of antioxidative Maillard reaction products from histidine and glucose to sadine products. Nippon Suisan Gakkaishi 54: 1409-1414 https://doi.org/10.2331/suisan.54.1409
  28. Je JY, Kim SK. 2006. Reactive oxygen species scavenging activity of aminoderivatized chitosan with different degree of deacetylation. Bioorg Med Chem 14: 5989-5994 https://doi.org/10.1016/j.bmc.2006.05.016
  29. Loft S, Fischer-Nielsen A, Jeding IB, Vistisen K, Poulsen HE. 1993. 8-Hydroxydeoxyguanosine as a urinary biomarker of oxidative DNA damage. J Toxicol Environ Health 40: 391-404 https://doi.org/10.1080/15287399309531806
  30. Je JY, Ahn CB, Oh MJ, Kang SY. 2009. Antioxidant activity of a red seaweed Polysiphonia morrowii extract. Food Sci Biotechnol 18: 124-129
  31. Du J, Gebicki JM. 2004. Proteins are major initial cell targets of hydroxyl free radicals. Int J Biochem Cell Biol 36: 2334-2343 https://doi.org/10.1016/j.biocel.2004.05.012
  32. Bahramikia S, Ardestani A, Yazdanparast R. 2009. Protective effects of four Iranian medicinal plants against free radical-mediated protein oxidation. Food Chem 115: 37-42 https://doi.org/10.1016/j.foodchem.2008.11.054

Cited by

  1. Protective effect of black raspberry seed containing anthocyanins against oxidative damage to DNA, protein, and lipid vol.53, pp.2, 2016, https://doi.org/10.1007/s13197-015-2094-7
  2. Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts ofRumex crispus vol.6, pp.5, 2012, https://doi.org/10.4162/nrp.2012.6.5.389
  3. Antioxidant and antiproliferative properties of water extract from Mahonia bealei (Fort.) Carr. leaves vol.49, pp.4, 2011, https://doi.org/10.1016/j.fct.2010.12.001
  4. Screening of the Physiological Activity of Solvent Extracts of Paulownia coreana Bark and Antioxidative Effect of the Extracts on an Edible Oil vol.22, pp.10, 2013, https://doi.org/10.5322/JESI.2013.22.10.1317
  5. Different Solvent Fractions ofAcanthopanax senticosusHarms Exert Antioxidant and Anti-Inflammatory Activities and Inhibit the Human Kv1.3 Channel vol.18, pp.4, 2015, https://doi.org/10.1089/jmf.2014.3182
  6. Antioxidant activity of Gardenia jasminoides Ellis fruit extracts vol.128, pp.3, 2011, https://doi.org/10.1016/j.foodchem.2011.03.090
  7. Antioxidant and nitric oxide release inhibition activities of methanolic extract from Clerodendrum cyrtophyllum Turcz vol.52, pp.3, 2011, https://doi.org/10.1007/s13580-011-0204-7
  8. Antioxidant Activity and Protection from DNA Damage by Water Extract from Pine (Pinus densiflora) Bark vol.17, pp.2, 2012, https://doi.org/10.3746/pnf.2012.17.2.116
  9. ANTIOXIDANT ACTIVITY OF MUME FRUCTUS EXTRACT vol.36, pp.2, 2012, https://doi.org/10.1111/j.1745-4514.2010.00529.x
  10. Comparison of Biological Activities of Synurus deltoides (Aiton) Nakai Under Different Shading Conditions vol.26, pp.6, 2013, https://doi.org/10.7732/kjpr.2013.26.6.686
  11. Protective effect of the methanolic extract from Duchesnea indica against oxidative stress in vitro and in vivo vol.31, pp.1, 2011, https://doi.org/10.1016/j.etap.2010.09.004
  12. Antioxidant Activity of Sorbus sibirica Fruit Extracts vol.900, pp.1662-8985, 2014, https://doi.org/10.4028/www.scientific.net/AMR.900.267
  13. Natural Plants Compounds as Modulators of Epithelial-to-Mesenchymal Transition vol.10, pp.None, 2009, https://doi.org/10.3389/fphar.2019.00715