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The Korean Society of Phycology (한국조류학회(藻類))
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Epistatic Relationships of Two Regulatory Factors During Heterocyst Development

  • Kim, Young-Saeng (Department of Biology,Kyungpook National University) ;
  • Kim, Il-Sup (Department of Biology,Kyungpook National University) ;
  • Shin, Sun-Young (Department of Biology,Kyungpook National University) ;
  • Kim, Hyun-young (Department of Biology,Kyungpook National University) ;
  • Kang, Sung-Ho (Division of Polar Biology & Ocean Sciences, Korea Polar Research Institute(KOPRI)) ;
  • Yoon, Ho-Sung (Department of Biology,Kyungpook National University)
  • Published : 2009.06.01
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Abstract

The filamentous cyanobacterium Anabaena sp. Strain PCC 7120 produces a developmental patten of single hete- rocysts separated by approximately 10 vegetative cells. Heterocysts differentiate from vegetative cells and are spe- cialized for nitrogen fixation. The patS gene, which encodes a small peptide that inhibits heterocyst differentiation, is expressed in proheterocysts and plays a critical role in establishing the heterocyst pattem. Another key regulator of heterocyst development is the hetR gene. hetR mutants fail to produce heterocysts and extra copies of hetR on a plas- mid cause a multiple contiguous heterocyst phenotype. To elucidate the relationship between these two counter act- ing factors in the genetic regulatory pathway during heterocyst differentiation, the expression patterns of a patS-gfp and a hetR-gfp fusion were examined in a patS deletion and a hetR deletion strain. The results, in combination with the result from a hetR and patS double deletion strain, suggest patS and hetR are mutually antagonistic and the bal- ance between these two factors in tow different cell types (heterocysts and vegetative cells) may be critical during the decision making process on their cell fates.

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References

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