Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of transgenic sweet potato producing human lactoferrin

인체 락토페린 생산 형질전환 고구마 개발

  • Min, Sung-Ran (Plant Systems Engineering Research Center) ;
  • Kim, Jae-Wha (Medical Genomics Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jeong, Won-Joong (Plant Systems Engineering Research Center) ;
  • Lee, Young-Bok (Dept. of Horticulture, Chungnam National University) ;
  • Liu, Jang R. (Plant Systems Engineering Research Center)
  • 민성란 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김재화 (유전체의학연구센터) ;
  • 정원중 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 이영복 (충남대 농업생명과학대학 원예학과) ;
  • 유장렬 (한국생명공학연구원 식물시스템공학연구센터)
  • Published : 2009.09.30
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Abstract

Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.

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References

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