Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
권호 표지

Fabrication of 3D PCL/PLGA/TCP Bio-scaffold using Multi-head Deposition System and Design of Experiment

다축 적층 시스템과 실험 계획법을 이용한 3차원 PCL/PLGA/ICP 바이오 인 공지지체 제작

  • Kim, Jong-Young (Department of Mechanical Engineering, POSTECH) ;
  • Yoon, Jun-Jin (Department of Pathology and Regenerative Medicine, School of Dentistry, KNU) ;
  • Park, Eui-Kyun (Department of Pathology and Regenerative Medicine, School of Dentistry, KNU) ;
  • Kim, Shin-Yoon (Department of Orthopedic Surgery, Kyungpook National University Hospital) ;
  • Cho, Dong-Woo (Department of Mechanical Engineering, POSTECH)
  • 김종영 (포항공과대학교 기계공학과 대학원) ;
  • 윤준진 (경북대학교 치의학과) ;
  • 박의균 (경북대학교 치의학과) ;
  • 김신윤 (경북대학교 정형외과) ;
  • 조동우 (포항공과대학교 기계공학과 대학원)
  • Published : 2009.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

In recent tissue engineering field, it is being reported that the fabrication of 3D scaffolds having high porous and controlled internal/external architectures can give potential contributions in cell adhesion, proliferation and differentiation. To fabricate these scaffolds, various solid free-form fabrication technologies are being applied. The solid free-form fabrication technology has made it possible to fabricate solid free-form 3D microstructures in layer-by-layer manner. In this research, we developed a multi-head deposition system (MHDS) and used design of experiment (DOE) to fabricate 3D scaffold having an optimized internal/external shape, Through the organization of experimental approach using DOE, the fabrication process of scaffold, which is composed of blended poly-caprolactone (PCL), poly-lactic-co-glycolic acid (PLGA) and tricalcium phosphate (TCP), is established to get uniform line width, line height and porosity efficiently Moreover, the feasibility of application to the tissue engineering of MHDS is demonstrated by human bone marrow stromal cells (hBMSCs) proliferation test.

Keywords

References

  1. Marra, K. G., Szem, J. W., Kumta, P. N., DiMilla, P. A. and Weiss, L. E., "In vitro analysis of biodegradable polymer blend/hydroxyl apatite composites for bone tissue engineering," J. of Biomedical Materials Research, Vol. 47, No. 3, pp. 324-335, 1999 https://doi.org/10.1002/(SICI)1097-4636(19991205)47:3<324::AID-JBM6>3.0.CO;2-Y
  2. Yang, D. -Y., Lim, T. W., Son, Y., Kong, H. -J., Lee, K. -S., Kim, D. -P. and Park, S. H., "Additive process using femto-second laser for manufacturing threedimensional nano/micro-struct ures," International Journal of Precision Engineering and Manufacturing, Vol. 8, No. 4, pp. 63-69, 2007
  3. Hutmacher, D. W., Schantz, T., Zein, I., Ng, K. W., Teoh, S. H. and Tan, K. C., "Mechanical properties and cell cultural response of polycaproplactonescaffolds designed and fabricated via fused deposition modeling," J. of Biomedical Materials Research Part A, Vol. 55, Issue 2, pp. 203-216, 2001 https://doi.org/10.1002/1097-4636(200105)55:2<203::AID-JBM1007>3.0.CO;2-7
  4. Lee, S. -J., Kang, H. -W., Kang, T. -Y., Kim, B., Lim, G., Rhie, J. -W. and Cho, D. -W., "Development of a scaffold fabrication system using an axiomaticapproach," J. Micromech. Microeng., Vol. 17, No. 1, pp. 147-153, 2007 https://doi.org/10.1088/0960-1317/17/1/019
  5. Wang, F., Shor, L., Darling, A., Khlil, S., Sun, W., Guceri, S. and Lau, A., "Precision extruding deposition and characterization of cellular poly-ecaprolactone tissue scaffolds," Rapid Prototyping Journal, Vol. 10, No. 1, pp. 42-49, 2004 https://doi.org/10.1108/13552540410512525
  6. Hutmacher, D. W., Sittinger, M. and Risbud, M. V., "Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems," Trends Biotechnol, Vol. 22, No. 7, pp. 354- 362, 2004 https://doi.org/10.1016/j.tibtech.2004.05.005
  7. Goldstein, S. A., "The mechanical properties of trabe cular bone: dependence on anatomic location and function," J. of Biomechanics, Vol. 20, No. 11-12, pp.1055-1061, 1987 https://doi.org/10.1016/0021-9290(87)90023-6
  8. Rezwan, K., Chen, Q. Z., Blaker, J. J. and Boccaccini, A. R., "Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissueengineering," Biomaterials, Vol. 27, Issue 18, pp. 3413-3431, 2006 https://doi.org/10.1016/j.biomaterials.2006.01.039