Anti-microbial Activity of Grapefruit Seed Extract and Processed Sulfur Solution against Human Skin Pathogens

피부질환 원인균에 대한 자몽종자추출물과 법제유황수의 항균 효과

  • Ha, Yu-Mi (IRIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Lee, Bo-Bae (IRIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Bae, Hee-Jung (IRIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Je, Kyoung-Mo (WellbuyKorea Co. Ltd.) ;
  • Kim, Soon-Rae (WellbuyKorea Co. Ltd.) ;
  • Choi, Jae-Suk (IRIS Center, Industry-Academic Cooperation Foundation, Silla University) ;
  • Choi, In-Soon (IRIS Center, Industry-Academic Cooperation Foundation, Silla University)
  • Published : 2009.01.30


This study was carried out to examine the antimicrobial effects of grapefruit seed extract (GSE) and processed sulfur solution (PSS) against human skin pathogens: Malassezia furfur, M. restricta, Propionibacterium arnes, Trichophyton mentagrophytes and T. rubrum. The antimicrobial effects of GSE and PSS were tested by agar diffusion method and micro broth dilution method. As the results, the MIC values of GSE against M. furfur, M. restricta, P. acnes, T. mentagrophytes and T. rubrum were 3.91, 3.91, 0.004, 0.024, and $0.012{\mu}l/ml$, respectively. The MIC values of PSS were 0.03, 0.03, 0.156, 0.003, and $0.012{\mu}l/ml$, respectively. Antimicrobial activity of skin care emulsion products containing 0.5% GSE and 0.5% PSS against human skin pathogens were 5.2, 4.3, 8.0, 9.5 and 12.8 mm, respectively. Refractive index, pH, viscosity and color value of skin care emulsions containing GSE and PSS were measured. According to these results, it was concluded that the GSE and PSS were the promising sources of antibacterial agent which could be useful for skin and hair care products as well as for the alternative medicine development in treatment of certain types of skin ailments.


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