생명과학회지 (Journal of Life Science)

  • 제19권11호
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  • Pages.1651-1657
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  • 2009
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

척수타박손상 후 트레드밀운동이 근 위축지연과 기능적 회복에 미치는 효과

The Effects of Treadmill Exercise on Inhibition of Soleus Muscle Atrophy and Improvement Functional Recovery after Spinal Cord Contusion in the Rats

  • 오명진 (한남대학교 생활체육학과) ;
  • 장문녀 (한남대학교 생활체육학과) ;
  • 서태범 (한남대학교 생활체육학과) ;
  • 김종오 (한남대학교 생활체육학과) ;
  • 변재종 (우송대학교 스포츠건강관리학부) ;
  • 윤진환 (한남대학교 생활체육학과) ;
  • 정일규 (한남대학교 생활체육학과)
  • Oh, Myung-Jin (Department of Sports Science, Hannam University) ;
  • Jang, Moon-Nyeo (Department of Sports Science, Hannam University) ;
  • Seo, Tae-Beom (Department of Sports Science, Hannam University) ;
  • Kim, Jong-Oh (Department of Sports Science, Hannam University) ;
  • Byun, Jae-Jong (Department of Health and Fitness Management, Woosong University) ;
  • Yoon, Jin-Hwan (Department of Sports Science, Hannam University) ;
  • Jeong, Il-Gyu (Department of Sports Science, Hannam University)
  • 발행 : 2009.11.30
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초록

본 연구는 흰쥐의 흉추 9번을 타박 손상시키고 1주간의 휴식 후 스트레스를 유발하지 않는 저강도 트레드밀운동을 7주동안 수행하여, 가자미근 위축지연 변화를 western blotting과 H&E 염색 그리고 면역형광염색법으로 관찰하였으며, BBB-검사를 이용하여 기능적 회복을 관찰하였다. 형태학적 변화의 결과 척수손상 후 트레드밀 운동에 의해 하지부위 가자미 근과 근섬유의 횡단면 크기가 증가하였으며, 근섬유 세포에서 IGF-I 단백질 발현이 증가하였다. 또한 생화학적인 결과 척수손상 후 트레드밀 운동에 의해 하지부위 골격근 IGF-I 단백질 발현이 증가하였으며, 행동학적 검사를 통해 트레드밀 운동그룹이 비 운동그룹보다 기능적 회복이 개선된 것을 확인하였다. 이러한 결과는 근 비대에 직접적으로 관여하는 IGF-I 단백질 발현량이 운동에 의해 증가하여 가자미근크기 증가와 같은 근 위축 지연을 확인하였으며, 이러한 변화를 통해 기능적 회복에 긍정적인 결과를 보여주었다. 따라서 운동은 척수손상 환자에게 효과적인 기능재활 치료방법이 될 수 있으며, 장애를 최소화하여 자발적인 일상생활을 도모할 수 있을 것이라 생각된다.

Physical activity and exercise can promote sensorimotor recovery from central nerve injury. It has been suggested that the functional recovery promoted by exercise training after spinal cord injury might be associated with insulin-like growth factor-I in the inflicted muscle. To investigate morphological and biochemical change of the soleus muscle after spinal cord injury, all tissues were used for H&E, immunofluorescence staining and Western blot. Also, BBB-test was used to evaluate behavioral improvement after spinal cord contusion. Thirty male Sprague-Dawley rats ($230{\pm}10\;g$; 7week in age) were assigned equally to three different groups; Normal (n=10), SCI (n=10), SCI+TMT (n=10). Every rat in SCI and SCI+TMT groups underwent laminectomy at T9 level and then contusion on the exposed spinal cord site in anesthetized condition. After one week-recovery from contusion, every rat in the SCI+TMT group exercised on a motorized treadmill for 30min/d, 5d/wk for 7wks. TMT followed by injury increased IGF-I induction levels in the soleus muscle and inhibited muscle atrophy. Behavioral scales for 4 and 8 weeks after spinal cord injury were improved in the SCI+TMT group compared to the SCI group. These results suggest that treadmill exercise after spinal cord injury might promote functional recovery along with muscle regrowth through the up-regulation of IGF-1 in muscle tissue.

키워드

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