Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

A Study on the Effect of Changchuldointanggami-bang(Cāngzhútáoréntāngjiāwèi-fāng) in Rheumatoid Arthritis

창출도인탕가미방(蒼朮桃仁湯加味方)이 류마토이드 관절염에 미치는 실험적 연구

  • Park, Eun-Sook (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoon, Il-Ji (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 박은숙 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 윤일지 (대전대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.07.15
  • Accepted : 2009.10.14
  • Published : 2009.10.31

⟨ Previous Next ⟩

Abstract

Objectives : This study was carried out to understand the immune responses of the Changchuldointanggami-bang(after referred to CDIT) on antioxidants, THP-1 cell and rheumatoid arthritis in Collagen-induced Arthritis(CIA) mice. Methods : The antioxidative effect of CDIT on DPPH free radical and SOD-like activity was measured. CDIT was administered to THP-1 cell, cytokine and mRNA associated with inflammation were measured. CDIT was orally administered to mice with arthritis by collagen II and then cytokine, PBMC in the serum were measured. Results : 1. The scavenging activity of CDIT on DPPH free radical was dose-dependent. 2. The effect of CDIT on SOD-like activity was dose-dependent. 3. The production of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ was decreased significantly in THP-1 cell. 4. The expression of $IL-1{\beta}$ mRNA, IL-6 mRNA, $TNF-{\alpha}$ mRNA were decreased significantly in THP-1 cell. 5. $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ were decreased significantly in the serum of CIA mice. 6. CD3+, CD4+, CD8+ were increased significantly, but CD3+/CD69+, CD3+/CD49b+, B220+/CD23+ were decreased significantly in PBMC of CIA mice. Conclusions : Taking all these observations into account, CDIT is considered to be effective in rheumatoid arthritis. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.

Keywords

References

  1. 한방재활의학과학회. 한방재활의학과학. 서울:군자출판사. 2003:91.
  2. 해리슨 번역 편찬위원회. 해리슨 내과학. 서울:정담출판사. 2006:2148-57.
  3. 巢元方. 諸病源候論. 北京:人民衛生出版社. 1982:5-8.
  4. 李梃. 編註醫學入門. 서울:大星文化社. 1989:369-71, 523.
  5. 王燾. 外臺秘要方. 서울:大星文化社. 1983:424
  6. 權宰植外. 痺證. 서울:鼎談. 1993:207-8, 214-5, 219-22, 270.
  7. TrenthamDE, TowensAS, KangAH. Autoimmunity totype II collagen : an experimental model of arthritis. J. Exp. Me. 1997;146:857-68
  8. 김영태, 이재동, 이윤호. 최근 RA와 관련된 임상 및 실험 논문의 경향. 대한침구학회지. 2002;19(5):190-4.
  9. 신경희. 蒼濟證方. 서울:삼장원. 1990:142
  10. 최정식, 송태원. 鷄血藤이 collagen으로 유발된 생쥐의 관절염억제에 관한 연구. 대한본초학회지. 2003;18(3):79-88.
  11. 서해경, 오민석, 김동희. 류마토이드 關節炎 患者 滑膜細胞에 대한 鷄血藤의 免疫反應. 동의생리병리학회지. 2003;17(3):780-6.
  12. 조영주. 豨薟이 炎症性 싸이토카인 및 류마티스양 關節炎에 미치는 影響. 大田大學校大學院. 2003.
  13. 安春載 外. 현호색, 유향 및 몰약 약침 자극이 진통효과에 미치는 영향. 대한침구학회지. 1994;11(1):145-58
  14. 배상철. 류마티스 관절염 치료의 최신지견. 가정의학회지. 2001;22(1):21-6.
  15. Peter Schenck, Stefan Schneider, Rolf Miehlke and Peter Prehm. Synthesis and degradation of hyaluronate by synovia from patients with rheumatoid arthritis. J. Rheumatol. 1995;22:400-5.
  16. 대한병리학회. 병리학. 서울:고문사. 1997:85, 1166-70,1672.
  17. 대한정형외과학회. 정형외과학. 서울:최신의학사. 2001:175-6.
  18. 龔信. 古今醫統. 南昌:江西科學技術出版社. 1990:284-5.
  19. 이승헌, 정수현, 김순중, 서일복. 秦艽, 威靈仙, 夏枯草 복합방이 Monosodium Iodoacetate로 유발된 흰쥐의 골관절염에 미치는 영향. 한방재활의학과학회지. 2008;18(1):75.
  20. 공상은, 오민석. 해동피복합방이 Collagen II 유발 관절염에 미치는 실험적 연구. 한방재활의학과학회지. 2008;18(2):1.
  21. 박진상, 한명금, 임양의, 이수경, 신병철, 권영달, 송용선. 생체외 류마토이드 관절염 실험 모델에서 청열사습탕의 효과. 한방재활의학과학회지. 2004;14(2):29.
  22. 설재욱, 신미숙, 최진봉. 마황강활탕이 Adjuvant 관절염에 미치는 실험적 효과. 한방재활의학과학회지. 2005;15(2):5.
  23. 손동우, 김순종, 서일복. 趁痛散이 흰쥐의 Adjuvant 유발 관절염 및 척수내 C-fos 발현에 미치는 영향. 한방재활의학과학회지. 2005;15(3):25.
  24. 全國韓醫科大學 本草學敎授 共編著. 本草學. 서울:永林社. 1995:196, 276, 306, 413, 445.
  25. 송규용, 이효정, 길재호, 김성훈. 자초의 항암성분 유도체 2-thioDMNQ-S160에 관한 연구. 동의생리병리학회지. 2002;16(3):542-6.
  26. 윤광재 외 3인. 자초뿌리의 성분 및 항균력에 관한 연구. 경희약대논문집. 1998;16:155-61.
  27. Chung HS, Kang M, Cho C, Park S, Kim H, Yoon YS, Kang J, Shin MK, Hong MC, Bae H. Inhibition of lipopolysaccharide and interferon-gamma-induced expression of inducible nitric oxide synthase and tumornecrosis factor-alphaby Lithospermi radixin mouse peritoneal macrophage. J Ethnopahrmacol. 2005;102(3):412-7. https://doi.org/10.1016/j.jep.2005.06.028
  28. Halliwell B, Gutteridge JM. Oxygen free radicals and iron in relation to biology and medicine : some problemsand concepts. Arch Biochem Biophys. 1986:246, 501-14.
  29. Kehrer JP. Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol. 1993:21-48
  30. Giatgen A. The Dual Role of Nitric Oxide in Islet ${\beta}$-cell. New Physiol Sci. 1999:14, 49-53.
  31. 방면호. 植物資源으로부터 天然抗酸化劑의 分離, 同定. 경희대학교 대학원. 1999:18.
  32. 최병기, 정세영, 박광식, 조정희. 활성산소와 질환. 서울:도서출판 신일상사. 2004:53-65, 166, 254.
  33. 이귀녕, 권오현. 임상병리파일 제3판. 서울:의학문화사. 2000:345-7, 399-404.
  34. Abul K. Abbas, Andrew H. Lichtman. Cellular and Molecular Immunology. 서울:범문사. 2004:185,423.
  35. 김창종, 서병세. 최신 병태생리학. 서울:신일서적. 2000:449.
  36. Punzi L, Calo L, Plebani M. Clinical significance of cytokine determination in synovial fluid. Crit Rev Clin Lab Sci. 2002;39:63-88. https://doi.org/10.1080/10408360290795448
  37. Harris ED Jr. Rheumatoidarthritis - Pathology and implication for therapy. New Engl. J. Med. 1990;322:1277-89 https://doi.org/10.1056/NEJM199005033221805
  38. Arend WP and Dayer JM. Cytokines and cytokine inhibitors of antagonists in rheumatoid arthritis. Arthritis Rheum. 1990;33:305-15. https://doi.org/10.1002/art.1780330302
  39. Saxne T, Palladino MA, Heinegard D, Talal N, Wollheim F. Detection of TNF-${\alpha}$ but not TNF-${\beta}$ in rheumatoid arthritis synovial fluid and serum. Arthritis Rheum. 1988;31:1041-5. https://doi.org/10.1002/art.1780310816
  40. Morita. Differential invitro effects of IL-4, IL-10 and IL-13 on proinflammatory cytokine production and fibroblast proliferation in rheumatoid synovium. Rheumatology International. 2001;20(2):49-54 https://doi.org/10.1007/s002960000074
  41. Okumoto H, Matsuda T and Turner M. The synovial expression and serum level of IL-6, IL-1, leukemia inhibitory factor and oncostatin inrheumatoid arthritis. Arthritis and Rheumatism. 1997;40:1096-105. https://doi.org/10.1002/art.1780400614
  42. Katherine L, Manson TL and Moore JM. Cytokine level in synovial fluid and serum. J Immunol Methods. 1994;138:47-56 https://doi.org/10.1016/0022-1759(91)90063-L
  43. Navarro S, Debili N, Bernaudin JF, Vainchenker W, Doly J. Regulation of the expression of IL-6 in human monocytes. J Immunol. 1989 ;142(12):4339-4.
  44. Feldman M. Development of anti-TNF therapy for rheumatoid arthritis. Nat Rev Immunol. 2002;2:364-71. https://doi.org/10.1038/nri802
  45. Feldman M, Maini RN, Bondeson J, Taylor P, Foxwell BM, Brennan FM. Cytokine blockade in rheumatoid arthritis. Adv Exp Med Biol. 2001;490:119-27.
  46. 정태호, 김정철, 김문규. 최신면역학 강의.대구:경북대학교 출판부. 1996:10, 22, 25, 77, 193, 200, 211-2, 215, 232-5, 248, 357.
  47. Choy EH, Panayi GS. Cytokine pathways and joint inflammation in rheumatoid arthritis. N Engl J Med. 2001;344:907-16 https://doi.org/10.1056/NEJM200103223441207
  48. Varbanova BB.et al. Clinic limmunology. New York:Mosby. 2001;4:13, 6:18.