Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Attenuation of Ischemia-Reperfusion Injury by Antioxidant Vitamins in a Pig Model of Renal Auto-Transplantation

돼지의 신장 자가이식에서 Ascorbic Acid와 Alpha-tocoperol 의한 허혈 및 재관류 손상의 감소

  • Kim, Myung-Jin (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Jae-Yon (College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Sung-Whan (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Chang-Sik (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
  • 김명진 (충남대학교 수의과대학) ;
  • 이재연 (충남대학교 수의과대학) ;
  • 조성환 (충남대학교 수의과대학) ;
  • 박창식 (충남대학교 동물자원과학부 형질전환복제돼지연구센터) ;
  • 전무형 (충남대학교 수의과대학) ;
  • 정성목 (충남대학교 수의과대학) ;
  • 김명철 (충남대학교 수의과대학)
  • Published : 2009.02.27
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Abstract

This study was to determine the effects of ascorbic acid and alpha-tocopherol on the attenuation of an ischemia-reperfusion injury (IRI) after renal auto-transplantation in a pig model. In the treatment group, three pigs were subjected to a renal auto-transplantation followed by the administration of ascorbic acid and alpha-tocopherol and the flushing of ascorbic acid plus hepa-saline solution. Otherwise, the control group used only flushing of hepa-saline solution. Blood samples were collected from these pigs for measurement of serum blood urea nitrogen (BUN) and creatinine values on the day before surgery and day 1, 3, 5 and 7 after surgery. The kidneys were taken for histopathological evaluation following euthanasia on day 14 after surgery. Serum creatinine and BUN values showed a significantly difference between control and treatment group on day 1, 3 and 5 (P<0.05). In histopathologic findings, treatment group showed less damage than that of the control group on the basis of renal tubular damage. As a result, this study suggests that the exogenous ascorbic acid and alpha-tocopherol pretreatment therapy with ascorbic acid irrigation-aspiration has a role of attenuation of renal I/R injury and recovery of renal function in a pig transplantation model.

본 연구의 목적은 돼지의 신장 자가이식에서 항산화제에 의한 허혈 및 재관류 손상의 감소에 대하여 ascorbic acid와 alpha-tocopherol이 미치는 영향을 평가하는 데 있다. 6두의 어린 돼지에 자가 신장 이식을 실시하였으며, 처치군에서는 수술 2일전 비타민 C와 E를 이틀 동안 전처치 하고, 그 뒤에 수술 중 비타민 C와 heparin이 첨가된 생리식염수를 절제되어 자가 이식할 신장에 관주하였다. 대조군에서는 수술 중 heparin이 첨가된 생리 식염수만을 절제되어 자가 이식할 신장에 관주하였다. 신장 기능을 평가하기 위하여 혈액 샘플을 채취하였으며, 수술 전, 수술 후 1, 3, 7, 14일에 혈청 creatinine과 BUN을 측정하였다. 그리고 병리조직 검사를 위해 14일 후 신장을 적출 보관하였다. 신장의 기능 검사에서 대조군과 처치군 사이에서 전체적인 유의성은 없었지만, 1일째, 3일째 또는 5일째에 두 그룹간의 유의적인 차이가 인정되었다 (p<0.05). 병리조직 검사 결과 처치군이 대조군 보다 더 적은 조직 손상의 정도를 보였다. 이러한 결과는, 비타민 C와 heparin을 이용한 신장의 관주 및 흡인의 과정이 신장의 허혈 및 재관류 손상을 감소시키는 데에 효과가 있었음을 시사하며, 이는 돼지의 신장 자가 이식에서 허혈 및 재관류의 손상을 감소시키며 신기능의 회복에 효과가 있음을 시사하는 바이다.

Keywords

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