Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Aureobasidium-Derived Soluble Branched (1,3-1,6) $\beta$-Glucan (Sophy $\beta$-glucan) Enhances Natural Killer Activity in Leishmania amazonensis-Infected Mice

  • Yatawara, Lalani (Department of Environmental Health Sciences, Kochi Medical School) ;
  • Wickramasinghe, Susiji (Department of Environmental Health Sciences, Kochi Medical School) ;
  • Nagataki, Mitsuru (Department of Environmental Health Sciences, Kochi Medical School) ;
  • Takamoto, Misa (Research and Development Division, Life Science Center, Sophy Incorporation) ;
  • Nomura, Haruka (Department of Environmental Health Sciences, Kochi Medical School) ;
  • Ikeue, Yasunori (Research and Development Division, Life Science Center, Sophy Incorporation) ;
  • Watanabe, Yoshiya (Research and Development Division, Life Science Center, Sophy Incorporation) ;
  • Agatsuma, Takeshi (Department of Environmental Health Sciences, Kochi Medical School)
  • Published : 2009.12.31
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Abstract

The $\beta$-glucans derived from yeast cell walls have been reported for having many immunomodulatory activities in vivo and in vitro. In this study, Aureobasidium-derived soluble branched (1,3-1,6) $\beta$-glucan (Sophy $\beta$-glucan) was checked for natural killer (NK) activity and for the production of IFN-$\gamma$ and IL-4 in Leishmania amazonensis infection. The main experiment was performed with a group of female C57BL/6 and BALB/c mice, orally supplemented with 5% of Sophy $\beta$-glucan and infected with promastogotes of L. amazonensis ($1\;{\times}\;10^7$) into the footpad. Increase in the footpad thickness with time was observed in BALB/c mice in spite of the oral Sophy $\beta$-glucan supplement, but it was less in C57BL/6 mice. The difference in overall mean footpad thickness between 'infection only' versus 'infection + glucan' groups was statistically significant (P < 0.001). High NK activity in C57BL/6 than BALB/c mice was observed in 'glucan only' group compared to the control group and also in 'infection + glucan' group compared to 'infection only' group. The difference in the NK activity among these groups was significant (P < 0.05). The IFN-$\gamma$ level increased at weeks 7 and 8 post-infection in C57BL/6 mice and was significantly high in 'infection + glucan' group compared to the 'infection only' group (P < 0.05). IL-4 levels did not increase up to detectable levels throughout the study. The results led a conclusion that Sophy $\beta$-glucan enhances NK activity and cellular immunity in L. amazonensis-infected mice.

Keywords

References

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