KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Melanin Inhibition, Anti-aging and Anti-inflammation Effects of Portulaca oleracea Extracts on Cells

쇠비름 추출물의 미백 및 항노화, 항염증 효과

  • Zhang, Rui (Department of Microbial Engineering, Konkuk University) ;
  • Lee, Hyun-Jin (Department of Microbial Engineering, Konkuk University) ;
  • Yoon, Yeong-Min (Department of Microbial Engineering, Konkuk University) ;
  • Kim, Su-Mi (Department of Microbial Engineering, Konkuk University) ;
  • Kim, Hyun-Sook (Department of Microbial Engineering, Konkuk University) ;
  • Li, Shun Hua (Department of Microbial Engineering, Konkuk University) ;
  • An, Sung-Kwan (Department of Microbial Engineering, Konkuk University)
  • 장뢰 (건국대학교 미생물공학과) ;
  • 이현진 (건국대학교 미생물공학과) ;
  • 윤영민 (건국대학교 미생물공학과) ;
  • 김수미 (건국대학교 미생물공학과) ;
  • 김현숙 (건국대학교 미생물공학과) ;
  • 리순화 (건국대학교 미생물공학과) ;
  • 안성관 (건국대학교 미생물공학과)
  • Published : 2009.08.29
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Abstract

The Portulaca oleracea (P. oleracea) is a popular herbal medicine in East Asia that was known to possess detoxification, antifebrile and antifungal effects. In the present study, we examined the biological activities of ethanol extracts of P. oleracea under various conditions with NIH3T3, B16F10, and MCF-7 cell line model systems. Extracts of P. oleracea (0.5 mg/ml) showed inhibition of expression of tyrosinase, but does not suppress either of TYRP-1 or DCT expression on B16F10 cells. Extracts of P. oleracea (2 mg/ml) showed anti-inflammatory effects on TNF-$\alpha$-stimulated NIH3T3/$NF{\kappa}B$-Luc cells and increase of the synthesis of collagen on NIH3T3 (wild type) cells. These results suggest that extracts of P. oleracea could be used as a functional biomaterial in developing a skin whitening agent and having the anti-inflammatory, anti-wrinkle, and anti-aging activities.

본 연구에서는 몇 가지 생물공학 기반 기술로서 이미 한방 화장품의 소재로 사용되고 있는 쇠비름 에탄올 추출액의 tyrosinase 저해, collagen 합성, 항염증 기대 효과, 항노화 대한 효능을 알아보기 위하여 B16F10 mouse melanoma cell, NIH3T3 mouse embryonic fibroblast, MCF-7 human breast adenocarcinoma cell에 쇠비름 추출액을 처리하여 실험하였다. 실험 결과, 쇠비름 에탄을 추출액 (0.5mg/mL)은 tyrosinase 발현을 억제하여 멜라닌 합성을 억제하며, 농도 의존적으로 NIH3T3 mouse fibroblast 세포의 collagen 합성을 촉진시켰다. 또한 2.0 mg/mL 농도의 쇠비름 추출액은 목단피보다 더욱 효과적으로 cytokine (TNF-$\alpha$)에 의한 NF-${\kappa}B$ 활성을 억제시켰다. Doxorubicin에 의한 과노화에서도 효과적인 항노화 작용을 하는 것으로 나타났다. Tyrosinase 합성을 억제하므로 미백에 대한 효과와 세포의 생장을 도와 collagen 합성을 촉진함으로서 노화방지에 효과적인 것으로 사료되며, TNF-$\alpha$에 의한 NF-${\kappa}B$의 활성화를 저해함으로서 염증반응 억제 효과도 기대 할 수 있다.

Keywords

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