수간주입한 아바멕틴의 곰솔과 잣나무 내 분포와 지속성

Persistence and Distribution of Trunk-Injected Abamectin in Pinus thunbergii and Pinus koraiensis Tissues

  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 김동수 (국립산림과학원 남부산림연구소) ;
  • 김철수 (산림청 산림인력개발원) ;
  • 조규성 (경상대학교 환경생명화학과) ;
  • 추호렬 (경상대학교 응용생물환경학과, 농업생명과학연구원) ;
  • 이동운 (경북대학교 생물응용학과)
  • Lee, Sang-Myeong (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Kim, Dong-Soo (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Kim, Chul-Su (Forest Human Resources Development Institute, Korea Forest Service) ;
  • Cho, Kyu-Seong (Division of Applied Life Science, Gyeongsang National University) ;
  • Choo, Ho-Yul (Department of Applied Biology and Environmental Sciences, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Lee, Dong-Woon (Department of Applied Biology, Institute of Agricultural Sciences, Kyungpook National University)
  • 발행 : 2009.09.30

초록

소나무재선충 방제제로 등록되어 사용되고 있는 abamectin 1.8% 유제를 소나무와 잣나무에 나무주사 한 뒤 수체내에서 잔류량을 측정하였다. 소나무 수체 내에서 검출한계는 $0.05\;mg\;kg^{-1}$이었으며 회수율은 가지에서는 $0.5\;mg\;kg^{-1}$ 처리시 90.9%였으며 수간에서는 93.1%였다. 수고 15 m의 곰솔에서 나무주사 된 abamectin 1.8% 유제는 처리 150일 후 소나무재선충의 매개충인 솔수염하늘소의 섭식 부분인 모든 가지부분에서 0.29-$0.73\;mg\;kg^{-1}$이 검출되었다. 평균 흥고직경 12.6 cm의 곰솔 천연림에서 abamectin 1.8% 유제의 수체 내 잔류량을 조사 한 결과 개체목별로 차이가 많았다. 곰솔의 수간부분에서는 하부와 중부에서는 abamectin 수간주사 후 시간의 경과에 따라 잔류량이 감소하는 경향을 보였으며 상부에서는 주사 30일 후에 $1.84\;mg\;kg^{-1}$이 검출되어 처리 15일 후의 $0.65\;mg\;kg^{-1}$보다 많았으며 100일과 180일 이후에는 검출한계 이하로 검출되었다. 가지부분에서는 하부에서는 처리 180일 후에 $0.183\;mg\;kg^{-1}$이 검출되었으며 중부에서는 $0.173\;mg\;kg^{-1}$이 검출되었다. 흉고직경 20 cm, 수고 9 m의 잣나무에서 가지부분의 abamectin 평균 검출량은 $0.80\;mg\;kg^{-1}$이었으며 수간에서는 $0.30\;mg\;kg^{-1}$이었고, 흉고직경 15 cm, 수고 6 m의 잣나무에서는 가지부분 $0.67\;mg\;kg^{-1}$이 검출되었고, 수간에서는 $0.36\;mg\;kg^{-1}$이 검출되었다.

The residues of abamectin 1.8% EC, resisted for control of pine wood nematode, Bursaphelenchus xylophilus in pine tree were surveyed in tissue of Pinus thunbergii and P. koraiensis after injection of a liquid formulation. Limits of detection of abamectin in tissue of P. thunbergii were $0.05\;mg\;kg^{-1}$ and mean recoveries at $0.5\;mg\;kg^{-1}$ trunk injection were 90.9% and 93.1% respectively in stem and trunk of P. thunbergii. Abamectin 1.8% EC, trunk injected in 15 m height P. thunbergii were detected in all stem (edible part of carrier insect of pine wood nematode, Monochamus alternatus) from 0.29 to $0.73\;mg\;kg^{-1}$ after 150 days injection. Amount of residue of abamectin 1.8% EC in 12.6 cm mean breast height diameter (DBH) P. thunbergii were variable depending on individual trees in natural forest. Amount of residues in lower and middle part of trunk were reduced with the passage of the injection time. In upper part of trunk were detected $1.84\;mg\;kg^{-1}$ on 30 days after injection however $0.65\;mg\;kg^{-1}$ on 15 days after injection and under detection limit on 100 and 180 days after injection in P. thunbergii. Bottom and middle parts of crown were detected $0.183\;mg\;kg^{-1}$ and $0.173\;mg\;kg^{-1}$ respectively on 180 days after injection in P. thunbergii. Mean residues of abamectin in crown and trunk were $0.80\;mg\;kg^{-1}$ and $0.30\;mg\;kg^{-1}$ on 170 days after trunk injection in 20 cm DBH and 9 m height P. koraiensis. Mean residues of abamectin in crown and trunk were $0.67\;mg\;kg^{-1}$ and $0.36\;mg\;kg^{-1}$ on 170 days after trunk injection in 15 cm DBH and 6 m height P. koraiensis.

키워드

참고문헌

  1. Dwinell, L. D. (1997) The pinewood nematode: regulation and mitigation. Ann. Rev. Phytopathol. 35:153-166 https://doi.org/10.1146/annurev.phyto.35.1.153
  2. Dwinell, L. D., and W. R. Nickle (1989) An overview of the pinewood nematode ban in North America. General Technical Report SE-55, North America Forestry Commission Publication No.2, Southeastern Forest Experimental Station, Forest Service, United States Department of Agriculture
  3. Kishi, Y. (1995) The pinewood nematode and Japanese pine sawyer. Thomas, Tokyo, p302
  4. Knowles, K, Y. Beaubien, M. J. Wingfield, F. A. Baker and D. W. French (1983) The pinewood nematode new in Canada. For. Chron. 59:40
  5. Kobayashi, F., A, Yamane and T. Ikeda (1984) The Japanese pine sawyer beetle as the vector of pine wilt disease. Ann. Rev. Entomol. 29:115-135 https://doi.org/10.1146/annurev.en.29.010184.000555
  6. Kweon, T. S., J. H. Lim, S. J. Shim, Y. D. Kwon, S. K Son, K Y. Lee, Y. T. Kim, J. W. Park, C. H. Shin, S. B. Ryu, C. K Lee, S. C. Shin, Y. J. Chung and Y. S. Park (2006) Distribution patterns of Monochamus altematus and M slatuarius (Coleoptera: Cerambycidae) in Korea. J. Korean For. Soc. 95:543-550
  7. Shin, S. C. (2008) Pine wilt disease in Korea, In Pine wilt disease (eds. Zhao, B. G., K Futai, J. R. Sutherland, and Y. takeuchi), Springer, Japan
  8. Steiner, G., and E. M. Buhrer (1934) Aphelenchoides xylophilous n. sp., a nematode associated with blue stain and other fungi in timber. J. Agric. Res. 48:949-951
  9. Takai K, T. Suzuki, and K Kawazu (2003a) Development and preventative effect against pine wilt disease of a novel liquid formulation of emamectin benzoate. Pest Manag Sci. 59:365-370 https://doi.org/10.1002/ps.651
  10. Takai K., T. Suzuki and K. Kawazu (2003b) Distribution and persistence of emamectin benzoate at efficacious concentrations in pine tissues after injection of a liquid formulation. Pest Manag. Sci. 60:42-48 https://doi.org/10.1002/ps.777
  11. Takai, K., T. Soejima, T. Suzuki and K. Kawazu (2000) Emamectin benzoate as a candidate for a trunk-injection agent against the pine wood nematode, Bursaphelenchus xylophilus. Pest Manag. Sci. 937-941
  12. Takeuchi, Y. (2008) Host fate following infection by the pine wood nematode. pp.235-249, In Pine wilt disease (eds. Zhao, B. G., K. Futai, J. R. Sutherland, and Y. takeuchi), Springer, Japan
  13. Yi, C. K., B. H. Yi, C. K., J. D. Park, S. I. Yang and K. H. Chang (1989) First finding of the pinewood nematode, Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle and its insect vector in Korea. Res. Rep. For. Res. Inst. 38:141-149
  14. 김동수, 이상명, 정영진, 최광식, 문일성, 박정규 (2003) 소나무재선충의 매개충인 솔수염하늘소 성충의 우화 생태. 한응곤지. 42:307-313
  15. 산림청 (2005) 소나무재선충병 효율적 방제 실무매뉴얼. 228pp
  16. 산림청 (2008) 산림병해충 방제 세부지침서. 15pp
  17. 한국작물보호협회 (2009) 2009 농약사용지침서. 1135pp