배추좀나방과 파밤나방의 pyrethroids약제에 대한 감수성 및 전기적 신경 반응 비교

Toxicological and Electrophysiological Activities of Pyrethroids between Larvae of Diamondback Moth, plutella xylostella and Beet Armyworm, Spodoptera exigua

본 실험은 배추좀나방과 파밤나방 유충에 처리방법에 따른 살충 효과를 토대로 신경계를 작용점으로 하는 pyrethroid계 약제를 이용하여 신경반응을 비교해 보았다. 배추좀나방과 파밤나방 모두 충체분무처리시 세 약제에서 50%이하의 살충률을 나타내었다. 엽면침지시 배추좀나방은 50ppm에서 deltamethrin은 100%, fenvalerate는 80%의 살충률을 보였으며 permethrin은 63.3%로 나타났다. 하지만 파밤나방은 모든 농도에서 control과 비슷한 낮은 활성을 보여주었다. 세 약제가 배추좀나방과 파밤나방 유충 신경에 어떤 영향을 미치는지 알아보기 위하여 전기생리반응을 통해 신경감수성을 살펴본 결과, 배추좀나방은 deltamethrin은 $10^{-7}M$, fenvalerate는 $10^{-5}M$, permethrin은 $10^{-3}M$에서 높은 반응을 보였으며, 파밤나방은 이 세 약제에서 모두 둔감한 반응결과를 얻었다.

Based on the insecticidal efficacy by insecticide treatment methods, neurophysiological responses were compared with the larvae of diamondback moth (DBM) and beet armyworm (BAW) using pyrethroids working on nervous system. By body spray method, all pyrethroids were showed lower insecticidal activity below 50% on the larvae of DBM and BAW. By leaf dipping method, DBM larvae were showed the insecticidal activity as 100% at 50 ppm deltamethrin, 80.0% at fenvalervate and 63.3% at permethrin. However, BAW larvae were showed lower insecticidal activity as similar as control in all concentration. In order to examine electrophysiological response on nervous system on DBM and BAW larvae when treated three insecticides, we investigated the voltage and reaction degree. The voltage of DBM were responded as high as $10^{-7}M$ in deltamethrin, $10^{-5}M$ in fenvalerate and $10^{-3}M$ in permethrin. However, those of BAW were showed dull responses with small variation of voltage to all three insecticides.