Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Angiotensin I Converting Enzyme Inhibitory Effects of Gelatin Hydrolysates Prepared from Tilapia mossambica Scales by Hot Water and Enzymatic Extraction

열수 및 효소적 가수분해로 제조된 틸라피아 비늘 젤라틴 가수분해물의 ACE 저해 활성

  • Ahn, Yong-Seok (Department of Food Bioengineering, Jeju National University) ;
  • Lee, Won-Woo (Department of Marine Life Science, Jeju National University) ;
  • Lee, Seung-Hong (Department of Marine Life Science, Jeju National University) ;
  • Ahn, Gin-Nae (Department of Marine Life Science, Jeju National University) ;
  • Ko, Chang-Ik (Department of Marine Life Science, Jeju National University) ;
  • Oh, Chang-Kyung (Department of Tourism Hotel Culinary Art, Jeju College of Technology) ;
  • Oh, Myung-Cheol (Department of Tourism Hotel Culinary Art, Jeju College of Technology) ;
  • Kim, Dong-Woo (Central Research Center, Natural F&P Co., Ltd.) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University) ;
  • Kim, Soo-Hyun (Department of Food Bioengineering, Jeju National University)
  • 안용석 (제주대학교 식품생명공학과) ;
  • 이원우 (제주대학교 해양생명과학과) ;
  • 이승홍 (제주대학교 해양생명과학과) ;
  • 안긴내 (제주대학교 해양생명과학과) ;
  • 고창익 (제주대학교 해양생명과학과) ;
  • 오창경 (제주산업정보대학 관광호텔조리과) ;
  • 오명철 (제주산업정보대학 관광호텔조리과) ;
  • 김동우 ((주)네추럴 F&P 중앙연구소) ;
  • 전유진 (제주대학교 해양생명과학과) ;
  • 김수현 (제주대학교 식품생명공학과)
  • Published : 2009.10.31
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Abstract

Fish scales have potential in functional food preparation due to their antioxidant and antihypertensive properties. We investigated the angiotensin I converting enzyme (ACE) inhibitory activity of Tilapia mossambica scale extracts. Hydrolysates of tilapia scales were prepared by enzymatic extraction using five proteases (${\alpha}$-chymotrypsin, Alcalase, Kojizyme, Protamex and trypsin) after scales were treated with hot water for 3 hr. Scale enzymatic hydrolysates prepared using both hot water and enzyme treatments exhibited elevated hydrolysis (about 25%-55%) compared to only enzyme treatment (about 15%-45%). Enzymatic hydrolysates (1 mg/mL) prepared by both hot water and enzyme treatments also showed significantly increased ACE inhibitory activities from about 20%-75%. The pattern of ACE inhibitory activities was similar to the degree of hydrolysis. Alcalase and ${\alpha}$-chymotrypsin hydrolysates displayed the highest ACE inhibitory activities ($IC_{50}$ = 0.83 mg/mL and 0.68 mg/mL, respectively). In addition, the ACE inhibitory effects of $IC_{50}$-chymotrypsin hydrolysates increased with decreasing molecular weight (5 kDa>, 10 kDa> and 30 kDa>), with the 5 kDa> fraction displaying the highest ACE inhibitory activity (about 89.9% and $IC_{50}$ = 0.1 mg/mL). We suggest that the peptide compounds of enzymatic hydrolysates prepared from tilapia scale enhances ACE inhibitory activity and might be useful as an antihypertensive material.

Keywords

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