Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Optimization of Supercritical Fluid Extraction of Tocotrienol from Grape Seed

초임계유체 추출을 이용한 포도씨 tocotrienol 추출조건 최적화

  • Kim, Kyeong-Mi (Department of Food Science and Technology, Chungbuk National University) ;
  • Woo, Koan Sik (Crop Post-Harvest Technology Division, National Institute of Crop Science) ;
  • Hwang, In-Guk (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Youn-Ri (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2009.02.28
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Abstract

In this study, supercritical carbon dioxide extraction (SFE) was utilized for the extraction of tocotrienol from grape seeds. The optimal conditions for vitamin E and tocotrienol extraction were determined via response surface methodology (RSM). Central composite design was utilized to assess the effects of oven temperature (30-$50^{\circ}C$, X1), operating pressure (17-25 MPa, X2), and extraction time (1-5 hr, X3) of supercritical fluid extraction. Vitamin E and tocotrienol contents were 8.65 mg/100 g and 7.88 mg/100 g at $40^{\circ}C$, 20MPa and 5 hr, respectively. The predicted extraction condition was validated via actual experimentation. The predicted extraction conditions were $40^{\circ}C$, 3.8 hr, and 20.7MPa. The vitamin E and tocotrienol contents under these conditions were 8.20 mg/100 g and 7.42 mg/100 g, respectively. The vitamin E and tocotrienol contents of solvent extraction with hexane were 8.18 mg/100 g and 7.24 mg/100 g, respectively.

포도가공 시 발생되는 부산물인 포도씨로부터 유용성분인 vitamin E 및 tocotrienol을 초임계 유체추출방법으로 최적화 하였다. 추출 온도(30-$50^{\circ}C$, $X_1$), 추출압력(17-25MPa, $X_2$) 및 추출시간(1-5 hr, $X_3$)을 변수로 반응표면 분석법으로 vitamin E 및 tocotrienol 함량을 측정하였다. 초임계 이산화탄소상에서 vitamin E 및 tocotrienol 함량은 추출온도, 압력 및 시간이 증가함에 따라 증가하였다 (plt;0.001). 각각의 추출조건 가운데 $40^{\circ}C$, 20 MPa 및 5 hr에서 vitamin E는 8.65 mg/100 g 그리고 tocotrienol은 7.88 mg/100 g으로 가장 높은 함량을 나타내었다. 반응표면분석에 의한 최적추출조건은 $40^{\circ}C$, 20.7MPa 및 3.8 hr으로 나타났으며, 이 때의 vitamin E 및 tocotrienol 함량은 각각 8.20 mg/100 g 및 7.42 mg/100 g으로 나타났다. Vitamin E 및 tocotrienol 함량에 대한 실측값은 통계적 예측값의 각각 99.27-106.10% 및 96.09-106.60%로 나타나 추출조건의 유효성이 인정되었다. 또한 유기용매 추출시 vitamin E 및 tocotrienol 함량은 각각 8.18 mg/100 g 및 7.24 mg/100 g이었다. 따라서 용매추출법과 초임계 이산화탄소 추출법을 비교했을 때 초임계 이산화탄소 추출법을 이용하는 것이 더 효과적으로 vitamin E 및 tocotrienol을 추출할 수 있을 것으로 판단되었다.

Keywords

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