Physiological Characteristics of Medicinal Plant Extracts for Use as Functional Materials in Seasoning Sauce for Pork Meat

돈육양념소스 소재 개발을 위한 한약재 추출물의 생리활성

  • Lee, Shin-Ho (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Kang, Kyung-Myung (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Park, Hyo-Jin (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Baek, Lag-Min (Department of Food Science and Technology, Catholic University of Daegu)
  • 이신호 (대구가톨릭대학교 식품가공학과) ;
  • 강경명 (대구가톨릭대학교 식품가공학과) ;
  • 박효진 (대구가톨릭대학교 식품가공학과) ;
  • 백락민 (대구가톨릭대학교 식품가공학과)
  • Published : 2009.02.28

Abstract

This study investigated the physiological characteristics of various medicinal plant extracts including Crataegi Fructus (CF), Perilla frutescens Britton var. acuta Kudo (PF), Geranium thunbergii sieb. et Zucc. (GT) and Polygonum multiflorum Thunberg (PM) for use in extending the shelf-life and improving the functional properties of seasoned pork. CF and GT showed a wide spectrum of antimicrobial activities against 5 kinds of Gram (+) bacteria and 5 kinds of Gram (-) bacteria. The antimicrobial activities of the extracts decreased as the heat treatment temperature increased between 60 and $121^{\circ}C$, but their activities remained relatively high following heat treatment. The total phenolic compound contents of the CF, PF, GT, PM extracts were 66.2, 29.0, 96.6 and 13.3 mg/g, respectively. The order of their DPPH radical scavenging activity was GT > CF > PF > PF. The nitrite scavenging activities of the CF, PF and GT extracts at 0.1% concentration were 10.5, 1.6 and 3.8%, respectively. The GT extract (99.7%) showed the highest degree of lipid rancidity inhibition compared to CF (90.3%), PF (88.9%) and PM (41.2%). After heat treatment for 15 min at $121^{\circ}C$, the DPPH radical scavenging activity of CF decreased but it increased in GT and PM. The nitrite scavenging activities of CF and GT decreased after heat treatment for 15 min at $121^{\circ}C$ but remained at relatively high levels. Also, degrees of lipid rancidity inhibition remained relatively high in PF (86.3%) and GT (99.8%) after heat treatment for 15 min at $121^{\circ}C$. These results suggest that CF and GT extracts could be used as ingredients in seasoning sauce to improve the shelf-life and functional properties of seasoned pork.

양념 돈육의 저장성 증진 및 기능성 보강용 돈육 양념소스 소재를 개발하기 위해 산사, 차조기, 현초, 하수오의 생리활성을 검토하였다. Gram positive 5균주와 Gram negative 5균주에 대한 항균활성을 검정한 결과 산사와 현초가 가장 넓은 항균 spectrum을 보였다. 열처리 온도가 높을수록 추출물의 항균 활성은 다소 감소하였으나 열처리 후에도 잔존하였다. 0.1% 산사, 차조기, 현초, 하수오 에탄올 추출물의 총 폴리페놀 함량은 각각 66.24 mg/g, 28.97 mg/g, 96.51 mg/g, 그리고 13.33 mg/g를 나타내어 현초가 가장 높았다. 현초의 DPPH 라디칼 소거능은 0.1, 0.5, 1.0%에서 각각 56.79, 92.24, 97.56%를 보였으며, 산사, 차조기, 현초, 하수오 추출물 중 DPPH 라디칼 소거능은 현초 추출물이 가장 높았으며 산사, 차조기, 하수오 순이었다. 추출물의 아질산염 소거능은 0.1% 농도에서 산사, 차조기, 현초 추출물은 각각 19.66, 1.66, 3.86%를 나타내어 산사가 가장 높았다. 각 추출물의 지방 산패 억제도는 산사 89.99%, 차조기 88.57%, 현초 99.71% 그리고 하수오는 43.21%로 현초가 가장 높았다. 추출물을 $121^{\circ}C$에서 15분 동안 열처리한 후 DPPH 라디칼 소거능은 산사는 감소하였으며, 차조기와 현초는 증가하였다. 산사, 차조기의 아질산염 소거활성은 열 처리 후에 다소 감소하기는 하였으나, 잔존하는 것으로 보였다. 열처리 후 지방 산패 억제도는 산사를 제외한 차조기, 현초에서는 다소 감소하였으나, 열처리 후에도 50${\sim}$99.8% 범위의 높은 활성을 보였다.

Keywords

References

  1. Ahn DH, Kim TH, Choi JI, Kim SN, Park ST. Studies on the improvement of pork meat quality using salt-fermented shrimp. J. Korean Soc. Food Sci. Nutr. 27: 482-488 (1998)
  2. KFDA. Official Book for Food. KFDA, Seoul, Korea. pp. 1-30 (2002)
  3. Moon JH, Ryu HS, Lee KH. Effect of garlic on the digestion of beef protein during storage. J. Korean Soc. Food Sci. Nutr. 20: 447-454 (1991)
  4. Oh DH. Studies on the quality of cured meat in the processing. PhD dissertation, Chonbuk National University, Jeonju, Korea (1986)
  5. Youn SK, Choi JS, Park SM, Ahn DH. Studies on the improvement of shelf-life and quality of vacuum-packaged seasoned pork meat by added chitosan during storage. J. Anim. Sci. Technol. 46:1023-1030 (2004)
  6. Han GJ, Shin DS, Kim JS, Cho YS, Jeong KS. Effects of propolis addition on quality characteristics of oriental medicinal seasoning pork. Korean J. Food Sci. Technol. 38: 75-81 (2006)
  7. Cho SH, Park BY, Yoo YM, Chae HS, Wyi JJ, Ahn CN, Kim JH, Lee JM, Kim YK, Yun SG. Physico-chemical and sensory characteristics of pork bulgogi containing ginseng saponin. Korean J. Food Sci. Anim. Resour. 22: 30-36 (2002)
  8. Jin SK, Kim IS, Hah KH, Hur SJ, Lyon HJ, Park KH, Bae DS. Changes of qualities in aerobic packed ripening pork using a Korea traditional seasoning during storage. J. Anim. Sci. Technol. 47: 73-82 (2005) https://doi.org/10.5187/JAST.2005.47.1.073
  9. Jin SK, Kim IS, Hah KH, Park KH, Kim IJ, Lee JR. Changes of pH, acidity, protease activity and microorganism on sauces using a Korean traditional seasoning during cold storage. Korean J. Food Sci. Anim. Resour. 26: 159-165 (2006)
  10. Lee HJ, Choi MS. Measurement of inhibitory activities on 3-hydroxy- 3-methylglutaryl CoA reductase and acyl-CoA: Cholesterol acyltransferase by various plant extracts in vitro. J. Korean Soc. Food Sci. Nutr. 28: 958-962 (1999)
  11. Oh DH, Ham SS, Park BK, Ahn C, Yu JY. Antimicrobial activities of natural medicinal herbs on the food spoilage or foodborne disease microorganisms. Korean J. Food Sci. Technol. 30: 957-963 (1998)
  12. Ban SS, Yoon HD, Shin OC, Shin YJ, Park CS, Park JH, Seo BI. The effects of Artemisiae Capillaris, Ponciri Fructus and Cartaegi Fructus in obese rats induced by high fat diet. Korean J. Herbol. 21: 55-67 (2006)
  13. Kim JS, Lee GD, Kwon JH, Yoon HS. Identification of phenolic antioxidative components in Crataegus pinnatifida Bunge. J. Korean Agri. Chem. Soc. 36: 154-157 (1993)
  14. Kang IH, Cha JH, Lee SW, Kim HJ, Kwon SH, Ham IH, Hwang BK, Whang WK. Isolation of antioxidant from domestic Cratageus pinnatifida Bunge leaves. Korean J. Pharmacon. 36: 121-128 (2005)
  15. Heo BG, Park SK. The origin of common name in Korean wild flower. Joongang Life Publishing Co., Seoul. Korea. p.170 (2002)
  16. Jung MH, Lee MY. Effect of Geranii herba water extract on gastric secretion and experimental ulceration in rats. J. Korean Pharm. Sci. 10: 1-7 (1989)
  17. Kim MH, Kang WW, Lee NH, Kwoen DJ, Choi UK. Antioxidant activities of extract with water and ethanol of Perilla frutescensvar. acuta kudo leaf. J. Korean Soc. Appl. Biol. Chem. 50: 327-333 (2007)
  18. Kim JY, Park CB, You DH, Yu YS, Noh SK, Ryu J. Studies on root cutting propagation culture for Polygonum multiflorum. Korean J. Med. Crop. Sci. 14: 502-503 (2006)
  19. Wang W, Wang JH, Shi TR. Effect of Polygonum multiflorum on the life-span and lipid metabolism in senile Japanese quails. Zhong Xi Yi Jie He Za Zhi 8: 223-224 (1988)
  20. Moon YG, Choi KS, Lee KJ, Kim KY, Heo MS. Screening of antioxidative and antibacterial activity from hot water extracts of indigenous plants, Jeju-island. Korean J. Biotechnol. Bioeng. 21:146-169 (2006)
  21. Cho MH. Effect of chitosan treatment on growth and quality of sunflower sprout. MS thesis, Catholic University of Daegu, Gyeongsan, Korea (2007)
  22. Blois MS. Antioxidant determination by the use of a stable freeradical. Nature 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  23. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agr. Bio.Chem. 51: 1333-1338 (1987) https://doi.org/10.1271/bbb1961.51.1333
  24. Kim DS, Ahn BW, Yeum DM, Lee DH, Kim SB, Park YH. Degradation of carcinogenic nitrosamine formation factor by natural food components : 1. Nitrite-scavenging effects vegetable extracts. Bull. Korean Fish. Soc. 20: 463-468 (1987)
  25. Buege JA, Aust SD. Microsomal lipid peroxidation. Method Enzymol. 105: 302-310 (1978)
  26. Kim MH, Lee NH, Lee MH, Kwon DJ, Choi UK. Antimicrobial activity of aurous ethanol extracts of Perilla frutescens var. acutaleaf. Korean J. Food Culture 22: 266-273 (2007)
  27. Jo DH, Min KJ, Cha CG. The antioxidant and antitumor effects of the extract of Bulnesia sarmientia. J. Food. Hyg. Saf. 22: 120-126 (2007)
  28. Lee JM, Chang PS, Lee JH. Comparison of oxidative stability for the thermally-oxidized vegetable oils using a DPPH method. Korean J. Food Sci. Technol. 39: 133-137 (2007)
  29. Kim J, Kim SA, Yun WK, Kim EJ, Woo MK, Lee MS. Antioxidative effect of ethanol extract for 5 kinds of spice. J. Korean Soc. Food Sci. Nutr. 33: 1426-1431 (2004) https://doi.org/10.3746/jkfn.2004.33.9.1426
  30. Gray JI, Dugan LR, Jr. Inhibition of N-nitrosamine formation in model food systems. J. Food Sci. 40: 981-984 (1975) https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  31. Kim DS, Ahn BW, Yeum DM, Lee DH, Kim SB, Park YH. Degradation of carcinogenic nitrosamine formation factor by natural food components: 1. Nitrite-scavenging effects of vegetable extracts. Bull. Korean Fish. Soc. 20: 463-468 (1987)
  32. Macrae R, Tobinson RK, Sadler MJ. Encyclopedia of food science, food technology and nutrition. Academic Press, New York, NY, USA. pp. 3240-3249 (1993)
  33. Lee JM, Ahn MS. A study on nitrite scavenging ability of teaextracts. Korean J. Diet. Culture 12: 567-572 (1997)