Journal of Pharmaceutical Investigation

  • 제39권1호
  • /
  • Pages.51-54
  • /
  • 2009
  • /
  • 2093-5552(pISSN)
  • /
  • 2093-6214(eISSN)
한국약제학회 (The Korean Society of Pharmaceutical Sciences and Technology)
권호 표지
DOI QR코드

DOI QR Code

βig-h3포함 키토산 상처 도포제의 섬유아세포 증식 및 상처치유 개선 능 평가

Effects of βig-h3/Chitosan Dressing on Dermal Fibroblast and Wound Healing

  • 조애리 (덕성여자대학교 약학대학) ;
  • 최희선 (덕성여자대학교 약학대학)
  • Cho, Ae-Ri (College of Pharmacy, Duksung Women's University) ;
  • Choi, Hee-Sun (College of Pharmacy, Duksung Women's University)
  • 발행 : 2009.02.20
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

${\beta}ig$-h3, is a TGF-${\beta}$-induced gene product, extracellular matrix protein with 68 kDa MW(683 amino acids) and has been known for its possible roles in cell adhesion, spreading, migration and proliferation. To minimize a proteolytic degradation of ${\beta}ig$-h3, ${\beta}ig$-h3 incorporated chitosan sponge was prepared and its effects on fibroblast adhesion and migration were investigated. And its wound healing efficacy was evaluated in deep 2nd degree burn rabbit ear wound model. ${\beta}ig$-h3 enhanced fibroblast adhesion and proliferation. In histological observation, a significant over-proliferation of epidermal regeneration was observed in ${\beta}ig$-h3/chitosan dressing applied wound while epidermal regeneration was not proceeded yet in chitosan only treated wound. ${\beta}ig$-h3/sponge dressing could enhance epidermal regeneration.

키워드

참고문헌

  1. J.S. Bae, S.H. Lee, J.E. Kim, J.Y Choi, R.W. Park, J.Y. Park, H.S. Park, Y.S Sohn, D.S. Lee, E.B Lee and I.S. Kim, $\beta$ig-h3 supports keratinocyte adhesion, migration, and proliferation through a3$\beta$1 integrin, Biochem.Biophysic.Res. Com., 294, 940-948 (2002). https://doi.org/10.1016/S0006-291X(02)00576-4
  2. J.E. Kim, H.W. Jeong, J.O. Nam, B.H. Lee, J.Y. Choi, R.W. Park, J.Y. Park and I.S. Kim, Identification of motifs in the fasciclin domains of the TGF-$\beta$ induced matrix protein βigh3 that interact with the avβ5 integrin, J. Biol. Chem., 277, 46159-46165 (2002). https://doi.org/10.1074/jbc.M207055200
  3. J.E. Kim, S.J. Kim, B.H. Lee, R.W. Park, K.S. Kim and I.S. Kim, Identification of motifs for cell adhesion within the repeated domains of TGF-$\beta$-induced gene, $\beta$ig-h3, J. Biol. Chem., 275, 30907-30915 (2000). https://doi.org/10.1074/jbc.M002752200
  4. J. Tang, H.W. Zhou, J.L. Jiang, X.M. Yang, Y.Li, H.X. Zhang, Z.N. Chen and W.P. Guo, $\beta$ig-h3 is involved in the HAb18G/CD147-mediated metastasis process in human hepatoma cells, Exp. Biol. Med., 232, 344-352 (2007).
  5. J.W. Ferguson, B.S. Thoma, M.F. Mikesh, R.H. Kramer, K. L. Bennett, A. Purchio, B. J. Bellard and R. G. LeBaron, The extracellular matrix protein $\beta$ig-h3 is expressed at myotendinous junctions and supports muscle cell adhesion, Cell and Tissue Research, 313, 93-105. https://doi.org/10.1007/s00441-003-0743-z
  6. M. Hardy, The biology of scar formation, Physical Therapy, 69, 1014-1024 (1989).
  7. J.S. Knabl, G Bayer, W. A. Bauer, I. Schewendenwein, P.F. Dado, C. Kucher, E. Turkof, B. Schossmann and G. Meissl, Controlled partial skin thickness burns: an animal model for studies of burn wound progression, Burns, 25, 229-235 (1999). https://doi.org/10.1016/S0305-4179(98)00172-7
  8. A. Cho and H. Moon, Controlled partial skin thickness burns: rabbit ear as a 2nd degree burn wound model for studies of topical therapy, J. Kor. Pharm. Sci., 36, 339-342 (2006).
  9. A. Cho Lee, H. Yong, A. Moon, J. Lee and K. Park, In vitro dermal fibroblast wound model to study mitogenic activity and cytokine levels, Proceedings of 8th World Biomaterials Congress, P-Sat-I-546, 2008.