Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Gene Cloning, Purification, and Characterization of a Cold-Adapted Lipase Produced by Acinetobacter baumannii BD5

  • Park, In-Hye (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Sun-Hee (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Yong-Seok (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Sang-Cheol (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Zhou, Yi (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Cheol-Min (School of Medicine, College of Medicine, Pusan National University) ;
  • Ahn, Soon-Cheol (School of Medicine, College of Medicine, Pusan National University) ;
  • Choi, Yong-Lark (Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University)
  • Published : 2009.02.28
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Abstract

Acinetohacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene was cloned and expressed in Escherichia coli BL21(trxB) as an inclusion body, which was subsequently solubilized by urea, and then purified using Ni-affinity chromatography. After being purified, the lipase was refolded by incubation at $4^{\circ}C$ in the presence of a 1:10 molar ratio of lipase:chaperone. The maximal activity of the refolded lipase was observed at a temperature of $35^{\circ}C$ and pH 8.3 when p-NP caprate(C10) was used as a substrate; however, 28% of the activity observed at $35^{\circ}C$ was still remaining at $0^{\circ}C$. The stability of the purified enzyme at low temperatures indicates that it is a cold-adapted enzyme. The refolded lipase was activated by $Ca^{2+},\;Mg^{2+},\;and\;Mn^{2+}$, whereas $Zn^{2+}\;and\;Cu^{2+}$ inhibited it. Additionally, 0.1% Tween 20 increased the lipase activity by 33%, but SDS and Triton X-100 inhibited the lipase activity by 40% and 70%, respectively.

Keywords

References

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