Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Water Extracts of Paecilomyces tenuipes Inhibit Cathepsin S-induced Adipocyte Differentiation in 3T3-L1 Cells

  • Published : 2009.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Cathepsin S is a cysteine protease that affects extracellular matrix remodeling. Recently, several studies have reported that cathepsin S is involved in obesity. Both mouse and human adipose cells produce this enzyme in the early phase of adipocyte differentiation, where it degrades fibronectin. Cathepsin S gene expression is elevated in the adipose tissue of obese mice as compared to that of lean mice. Paecilomyces tenuipes water extracts (PTW) are shown to have an inhibitory effect on cathepsin S activity. In this study, Z-Val-Val-Arg-MCA was used as a cathepsin S-specific substrate in order to examine inhibitory effect of PTW. Supplementing 3T3-L1 cell media with PTW clearly reduced lipid droplet accumulation and cathepsin S-induced adipogenesis. Furthermore, PTW decreased weight gain, subcutaneous adipose tissue growth, the level of serum triglyceride, and total cholesterol in mice fed a high-fat diet. These data suggest that PTW work against adipose cathepsin S and presumably contribute to anti-obese activities.

Keywords

References

  1. Borchers AT, Stem JS. Hackman RM, Keen CI, Gershwin ME. Mushrooms, tumors, and immunity. P. Soc. Exp. BioI. Med. 221:281-293 (1999) https://doi.org/10.1046/j.1525-1373.1999.d01-86.x
  2. Liu F, Ooi VEC, Liu WK, Chang ST. Immunomodulation and antitumor activity of polysaccharide-protein complex from the culture filtrates of a local edible mushroom, Tricholoma lobayense. Gen. Pharmacol. 27: 621-624 (1996) https://doi.org/10.1016/0306-3623(95)02058-6
  3. Lee JH, Cho SM, Song KS, Hong ND, Yoo ID. Characterization of carbohydrate-peptide linkage of acidic heteroglycopeptide with immuno-stimulating activity from mycelium of Phellinus linteus. Chem. Pharm. Bull. 44: 1093-1095 (1996) https://doi.org/10.1248/cpb.44.1093
  4. Park E, Park NS, Park HR, Jin BR, Lee SM. Fruiting body extracts of Paecilomyces tenuipes ameliorate lipid and antioxidant metabolism in rats fed a high fat-cholesterol dict. Food Sci. Biotechnol. 15: 710-714 (2006)
  5. Park E, Jeon GI, Park NS, Jin BB, Lee SM. Antigenotoxic effect of Paecilomyces tenuipes cultivated on soybeans in a rat model of 1, 2-dimethylhydrazine-induced colon carcinogenesis. Food Sci. Biotechnol. 16: 1064-1068 (2007)
  6. Nam KS, Jo YS, Kim YH, Hyun JW, Kim HW. Cytotoxic activities of acetoxyscirpenediol and ergosterol peroxide from Paecilomyces tenuipes. Life Sci. 69: 229-237 (2001) https://doi.org/10.1016/S0024-3205(01)01125-0
  7. Nilanonta C, Isaka M, Kittakoop P, Palittapongampim P, Kamchonwongpaisan S, Pittayakhajonwut D, Tanticharoen M, Thebtaranonth Y. Antimycobacterial and antiplasmodial cyclodepsipeptides from the insect pathogenic fungus Paecilomyces tenuipes BCC1416. Planta Med. 66: 756-758 (2000) https://doi.org/10.1055/s-2000-9776
  8. Lee SM, Park NS, Jin BR, Kang HS, Jung JH, Park E. Effects of Paecilomyces tenuipes cultivated in egg yolk on lipid metabolism in rats on high fat-cholesterol diet. J. Med. Food 9: 214-222 (2006) https://doi.org/10.1089/jmf.2006.9.214
  9. Taleb S, Cancello, R, Clement K, Lacasa D. Cathepsin S promotes human preadipocyte differentiation: Possible involvement of fibronectin degradation. Endocrinology 147: 4950-4959 (2006) https://doi.org/10.1210/en.2006-0386
  10. Taleb S, Lacasa D, Bastard JP, Poitou C, Cancello R. Pelloux V, Viguerie N, Benis A, Zuker JD, Bouillot JL, Coussieu C, Basdevant A, Langin D. Clement K. Cathepsin S, a novel biomarker of adiposity: Relevance to atherogenesis. FASEB J. 19: 1540-1542 (2005) https://doi.org/10.1096/fj.05-3673fje
  11. Taleb S, Cancello R. Poitou C, Rouault C, Sellam P, Levy P, Bouilot JL, Coussieu C, Basdevant A, Guerre-MilIo M, Laeasa D, Clement K. Weight loss reduces adipose tissue cathepsin S and its circulating levels in morbidly obese women. J. Clin. Endocr. Metab. 91: 1042-1047 (2006) https://doi.org/10.1210/jc.2005-1601
  12. Shindo K, Suzuki H, Okuda T. Paecilopeptin, a new cathepsin S inhibitor produced by Paecilomyces carneus. Biosei. Biotech. Bioch. 66: 2444-2448 (2002) https://doi.org/10.1271/bbb.66.2444
  13. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18: 499-502 (1972)
  14. Wang B, Sun J, kitamoto S, Yang M, Grubb A, chapman HA, Kalluri R, Shi GP. Cathepsin S controls angiogenesis and tumor growth via matrix-derived angiogenic factors. J. Biol. Chem. 281:6020-6029 (2006) https://doi.org/10.1074/jbc.M509134200
  15. Sukhova GK, Zhang Y, Pan JH, Wada Y, Yamaoto T, Naito M, Kodama T, Tsimikas S, Witztum JL, Lu ML, Sakara Y, Chin MT, Libby P, Shi GP. Deficiency of cathepsin Sreduces atherosclerosis in LDL receptor-deficient mice. J. Clin. Invest. 111: 897-906 (2003) https://doi.org/10.1172/JCI200314915
  16. Nadler ST, Stoehr JP, Schueler KL, Tanimoto G, Yandell BS, Attie AD. The expression of adipogenic genes in decreased in obesity and diabetes mellitus. P. Natl. Acad. Sci. USA 97: 11371-11376 (2000) https://doi.org/10.1073/pnas.97.21.11371
  17. Yamaguchi Y, Kagota S, Nakamura K, Shinozuka K, kunitomo M. Inhibitory effects of water extracts from fruiting bodies of cultured Cordyceps sinensis on raised serum lipid peroxide levels and aortic cholesterol deposition in atherosclerotic mice. Phytother. Res. 14:650-652 (2000) https://doi.org/10.1002/1099-1573(200012)14:8<650::AID-PTR675>3.0.CO;2-0
  18. Yuan Z, He P, Takeuchi H. Ameliorating effects of water-soluble polysaccharides from woody ear (Auricularia auricular-judae Quel.) in genetically diabetic KK-Ay mice. J. Nutr. Sci. Vitaminol. 45: 151-159 (1999) https://doi.org/10.3177/jnsv.45.1_151
  19. Cha JY, Jun BS, Yoo KS, Hahm JR, Cho YS. Fermented chaga mushroom (Inonotus obliquus) effects on hypolipidemia and hepatoprotection in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Food Sci. Biotechnol. 15: 122-127 (2006)
  20. Choi SB, Park CH, Choi MK, Jun DW, Park S. Improvement of insulin resistance and insulin secretion by water extracts of Cordyceps miliatris, Phellinus linteus, and Paecilomyces tenuipes in 90% pancreatectomized rats. Biosci. Biotech. Bioch. 68: 2257-2264 (2004) https://doi.org/10.1271/bbb.68.2257
  21. Kim HS, Roh YJ, Choe M. Cordyceps miliatris increases hepatic glucokinase activities. J. Korean Soc. Food Sci. Nutr. 34: 158-161 (2004) https://doi.org/10.3746/jkfn.2005.34.2.158
  22. Kim HS, Choe M. Hypoglycemic effect of Paecilomyces japonica in NIDDM patients. J. Korean Soc. Food Sci. Nutr. 34: 821-824 (2005) https://doi.org/10.3746/jkfn.2005.34.6.821
  23. Koh JB, Choi MA, Effect of Paecilomyces japonica on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 32: 238-243 (2003) https://doi.org/10.3746/jkfn.2003.32.2.238