Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of a Compound Extract from Agrimonia pilosa Ledeb, Grifola umbellata (pers.) Pilat, and Gambogia on Human Gastric Carcinoma MGC-803 Cells

  • Zhao, Li (Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University) ;
  • Zhang, Xiao-Nan (Department of Pharmacology, the Fourth Military Medical University) ;
  • Gu, Hong-Yan (Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University) ;
  • Wang, Jia (Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University) ;
  • Tao, Lei (Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University) ;
  • Mu, Rong (Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University) ;
  • Guo, Qing-Long (Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University)
  • Published : 2009.02.28
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Abstract

Three traditional Chinese medicines, Agrimonia pilosa Ledeb, Grifola umbellata (pers.) Pilat, and Gambogia, are combined to form a compound extract, AGC. In this study, the in vitro and in vivo inhibitory effects of AGC on human gastric carcinoma MGC-803 cells were demonstrated, and the molecular mechanisms underlying these effects are investigated. Our results indicate that AGC inhibited MGC-803 cell growth in a dose-dependent manner as measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, with an $IC_{50}$ of about $6.045{\pm}0.69{\mu}g/mL$. In vivo, AGC inhibited growth of human gastric carcinoma in xenograft tumors in nude mice, and the inhibitory rate reached 55.2% at 300 mg/kg. The pro-apoptotic activity of AGC was attributed to its ability to decrease the expression of Bcl-2 and Pro-caspase3 and increase the expression of Bax. These results demonstrate that AGC can effectively induce programmed cell death and may be a promising anti-tumor drug in human gastric carcinoma.

Keywords

References

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