Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지

ANTIMICROBIAL ACTIVITY OF ARTEMISIA SPECIES AGAINST CLINICALLY ISOLATED STREPTOCOCCUS MUTANS

임상 분리된 Streptococcus mutans에 대한 Artemisia species의 항균 활성도

  • Seo, Jung-Ah (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Jae-Gon (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Kim, Mi-Ah (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Baik, Byeong-Ju (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Yang, Yeon-Mi (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University) ;
  • Jeong, Jin-Woo (Department of Pediatric dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University)
  • 서정아 (전북대학교 치의학전문대학원 및 구강생체과학연구소) ;
  • 김재곤 (전북대학교 치의학전문대학원 및 구강생체과학연구소) ;
  • 김미아 (전북대학교 치의학전문대학원 및 구강생체과학연구소) ;
  • 백병주 (전북대학교 치의학전문대학원 및 구강생체과학연구소) ;
  • 양연미 (전북대학교 치의학전문대학원 및 구강생체과학연구소) ;
  • 정진우 (전북대학교 치의학전문대학원 및 구강생체과학연구소)
  • Published : 2009.11.30
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Abstract

Streptococcus mutans plays a major role in the formation of dental plaque and it is considered one of the important pathogens in the development of dental caries. Established dental plaque can be more resistant to antimicrobial agents and offers nutrient rich and relatively stable cariogenic conditions for bacteria. Thus bacteria growing in dental plaque have strong resistance to antimicrobial agents and they are not removed easily by the flow of saliva. Many researchers have been performed using natural materials, especially herbal extracts to prevent dental plaque. However, the strains of mutans streptococci used in the researches were not from Koreans. Therefore, it would be necessary to evaluate the antimicrobial activity of herbal extracts against clinical isolates of Streptococcus mutans isolated from saliva of Koreans living in Jeollabuk-do. For this study four clinical isolates were isolated from saliva samples of seventeen Korean people to investigate whether essential oils of Artemisia spp. have antimicrobial activity against them including four reference strains of Streptococcus mutans. Minimum inhibitory concentrations, Minimum bactericidal concentrations and time kill studies were performed and the results showed that Artemisia lavandulaefolia DC, Artemisia scoparia, and Artemisia capillaries have antimicrobial activity against the Streptococcus mutans clinical isolates and reference strains.

Streptococcus mutans는 Mutans streptococci 그룹에 속하는 치아우식 유발균으로 알려져 있다. 국내 자생쑥 종의 추출물이 이러한 치아우식을 유발하는 Streptococcus mutans균에 대해 활성 억제 효과가 있다는 것이 표준균주를 이용한 실험에서 밝혀져 있으며, 본 연구에서는 환자에서 분리된 S. mutans를 중심으로 쑥의 항균 효과를 알아보았다. 네 가지 임상 분리균주; Ch, L1, L2, P와 네 가지 표준균주: KCTC (Korean Culture for Type Collection) 3298, KCTC 3605 (ATCC 25175), KCTC 3306, Streptococcus mutans GS5를 이용하여 세 가지 종류의 쑥; 참쑥(Artemisia lavandulaefolia DC), 비쑥(Artemisia scoparia), 사철쑥(Artemisia capillaries)의 효능을 검증하였다. 항균활성은 세 가지 종류의 쑥 추출물의 Streptococcus mutans에 대한 최소 억제농도(MIC)와 최소살균농도(MBC), time-kill studies를 통해 알아보았다. 최소억제농도는 표준균주에 대해 각각 참쑥이 0.8 ug/mL, 비쑥이 0.8-1.6 ug/mL, 사철쑥이 0.2-25.6 ug/mL이 었으며, 분리된 균주에 대해서는 참쑥이 0.4-0.8 ug/mL, 비쑥이 0.4-0.8 ug/mL, 사철쑥 1.6-6.4 ug/mL이었다. 최소살균농도는 표준균주에 대해서 참쑥이 1.6-6.4 ug/mL, 비쑥이 6.4-12.8 ug/mL이였다. 분리균주에 대해서는 참쑥이 0.8-3.2 ug/mL, 비쑥이 0.8-3.2 ug/mL, 사철쑥은 6.4-12.8 ug/mL이었다. Time-kill assay에서 KCTC 3298이 4 hrs에 100% 살균되었으며, GS5도 거의 100% 살균되었고 L1과 L2 P는 0.5 hrs에 99.90% 살균되었다. 0.5 hrs에 KCTC 3298와 GS5는 99.99% 이상 살균되었고, 모든 균주들이 2.5 hrs에 99.99% 살균되었다. 전체적인 실험 결과 세 가지 종류의 쑥은 표준 균주 및 네 가지 분리 균주 모두에서 항균 효과가 있음을 확인하였다.

Keywords

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