Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production, Purification, and Characterization of Taxol and 10-DABIII from a new Endophytic Fungus Gliocladium sp. Isolated from the Indian Yew Tree, Taxus baccata

  • Sreekanth, D. (Biochemical Sciences Division, National Chemical Laboratory) ;
  • Syed, A. (Biochemical Sciences Division, National Chemical Laboratory) ;
  • Sarkar, S. (Combi-Chem Bioresource Center, Division of Organic Chemistry Technology, National Chemical Laboratory) ;
  • Sarkar, D. (Combi-Chem Bioresource Center, Division of Organic Chemistry Technology, National Chemical Laboratory) ;
  • Santhakumari, B. (Center for Material Characterization, National Chemical Laboratory) ;
  • Ahmad, A. (Biochemical Sciences Division, National Chemical Laboratory) ;
  • Khan, M.I. (Biochemical Sciences Division, National Chemical Laboratory)
  • Published : 2009.11.30
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Abstract

We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded $10\;{\mu}g$ of taxol and $65\;{\mu}g$ of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer).

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