DOI QR코드

DOI QR Code

Evaluation of Phytochemical Composition and Antioxidant Capacity in Various Leafy Vegetables

  • Lee, Joon-Hee (Health Metrology Center, Division of Quality of Life, Korea Research Institute of Standards and Science) ;
  • Lee, Sun-Young (Department of Food Science and Nutrition, The Catholic University of Korea) ;
  • Lee, Hyang-Jung (Department of Food Science and Nutrition, The Catholic University of Korea) ;
  • Kim, Young-Jun (Department of Agricultural Biotechnology, Seoul National University, R&D Food Safety Center, OTTOGI Corporation) ;
  • Koh, Kyung-Hee (Department of Food Science and Nutrition, The Catholic University of Korea)
  • Published : 2009.03.31

Abstract

Current study investigated phytochemical compositions and antioxidant capacity of Korean leafy vegetables including chajogi, gomchwi, meowi and sseumbagwi. ${\beta}$-carotene, total soluble polyphenol, total flavonoids contents were determined, and antioxidant capacity were evaluated by various methods. ${\beta}$-carotene, total soluble polyphenol, total flavonoids contents in gomchwi were significantly higher (as much as 19 folds) compare to other vegetables (p<0.05). Sseumbagwi extract ($100{\mu}g/mL$) was removed 78.6% of superoxide radicals in xanthin-xanthin oxidase system measured by EPR. Gomchwi showed the highest nitrate scavenging activity as 94.3% at pH 1.2. In SOD-like activity, chajogi, gomchwi, and meowi were evaluated in the range of $12.6{\sim}24.5%$. All samples were revealed to prevent the reaction of ferrous chloride during 9 days, which were comparable ability with 125 mg/100 g of ${\alpha}$-tocopherol. The rich phytochemical contents of gomchwi and sseumbagwi influenced to high antioxidant capacity.

Keywords

References

  1. Levi F. 1999. Cancer prevention: epidemiology and perspectives. Eur J Cancer 35: 1046-1058 https://doi.org/10.1016/S0959-8049(99)00094-5
  2. Knekt P, Kumpulainen J, Jarvinen R, Fissanen H, Heliovaara M, Reunanen A, Hakulinen T, Aromaa A. 2002. Flavonoid intake and risk of chronic diseases. Am J Clin Nutr 76: 560-568 https://doi.org/10.1093/ajcn/76.3.560
  3. Meyer AS, Heinonen M, Frankel EN. 1998. Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin, and ellagic acid on human LDL oxidation. Food Chem 61: 71-75 https://doi.org/10.1016/S0308-8146(97)00100-3
  4. Talcott ST, Lee JH. 2002. Ellagic acid and flavonoid antioxidant content of muscadine wine and juice. J Agric Food Chem 50: 3186-3192 https://doi.org/10.1021/jf011500u
  5. Svobodova A, Psotova J, Walterova D. 2003. Natural phenolics in the prevention of UV-induced skin damage. A review. Biomed Prap Med Fac Univ Palacky Olomouc Czech Repub 147: 137-145 https://doi.org/10.5507/bp.2003.019
  6. Hsu S. 2005. Green tea and the skin. J Am Acad Dermatol 52: 1049-1059 https://doi.org/10.1016/j.jaad.2004.12.044
  7. Cao G, Russell RM, Lischner N, Prior RL. 1998. Serum antioxidant capacity is increased by consumption of strawberries, spinach, red wine or vitamin C in elderly women. J Nutr 545: 51-64
  8. Ramassamy C. 2006. Emerging role of polyphenolic compounds in the treatment of neurodegenerative diseases: A review of their intracellular targets. Eur J Pharmacol 545: 51-64 https://doi.org/10.1016/j.ejphar.2006.06.025
  9. Chereau-Boudet I, de Chazeron I, Derost P, Ulla M, Lemaire JJ, Durif F, Llorca PM. 2007. Stimulation of the subthalamic nucleus in Parkinson's disease and mood disorders, one-year follow-up. Eur Psychiatry 22: 227-341 https://doi.org/10.1016/j.eurpsy.2007.01.759
  10. Takeda H, Tsuji M, Matumiya T, Kubo M. 2002. Identification of rosmarinic acid as a novel antidepressive substance in the leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba). Japanese J Psychopharm 22: 15-22
  11. Kawahata T, Otake T, Mori H, Kojima Y, Oishi I, Oka S, Fukumori Y, Sano K. 2002. A novel substance purified from Perilla frutescens Britton inhibits an early stage of HIV-1 replication without blocking viral adsorption. Antivir Chem Chemother 13: 283-288 https://doi.org/10.1177/095632020201300503
  12. Ueda H, Yamazaki C, Yamazaki M. 2002. Luteolin as an anti-inflammatory and anti-allergic constituent of Perilla frutescens. Biol Pharm Bull 25: 1197-1202 https://doi.org/10.1248/bpb.25.1197
  13. Banno N, Akihisa T, Tokuda H, Yasukawa K, Higashihara H, Ukiya M, Watanabe K, Kimura Y, Hasegawa J, Nishino H. 2004. Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory and antitumor-promoting effects. Biosci Biotechnol Biochem 68: 85-90 https://doi.org/10.1271/bbb.68.85
  14. Takano H, Osakabe N, Sanbongi C, Yanagisawa R, Inoue K, Yasuda A, Natsume M, Baba S, Ichiishi E, Yoshikawa T. 2004. Extract of Perilla frutescens enriched for rosmarinic acid, a polyphenolic phytochemical, inhibits seasonal allergic rhinoconjunctivitis in humans. Expe Biol Med (Maywood) 229: 247-254 https://doi.org/10.1177/153537020422900305
  15. Lin CS, Kuo CL, Wang JP, Chenga JS, Huanga ZW, Chena CF. 2007. Growth inhibitory and apoptosis inducing effect of Perilla frutescens extract on human hepatoma HepG2 cells. J Ethnopharmacol 112: 557-567 https://doi.org/10.1016/j.jep.2007.05.008
  16. Choi EM, Kim YH. 2008. A preliminary study of the effects of an extract of Ligularia fischeri leaves on type II collagen-induced arthritis in DBA/1J mice. Food Chem Toxicol 46: 375-379 https://doi.org/10.1016/j.fct.2007.08.018
  17. Mizushina Y, Ishidoh T, Kamisuki S, Nakazawa S, Takemura M, Sugawara F, Yoshida H, Sakaguchi K. 2003. Flavonoid glycoside: a new inhibitor of eukaryotic DNA polymerase A and a new carrier for inhibitor-affinity chromatography. Biochem Biophys Res Commun 301: 480-487 https://doi.org/10.1016/S0006-291X(02)03083-8
  18. Chung HS, Jeong HJ, Han MJ, Park ST, Seong KK, Baek SH, Jeong DM, Kim MJ, Kim HM. 2002. Nitric oxide and tumor necrosis factor-a production by Ixeris dentate in mouse peritoneal macrophages. J Ethnopharmacol 82: 217-222 https://doi.org/10.1016/S0378-8741(02)00188-5
  19. Swain T, Hillis WE. 1959. The phenolic constituents of Prumus domestica. I. The quantitative analysis of phenolic constituents. J Sci Food Agric 10: 63-68 https://doi.org/10.1002/jsfa.2740100110
  20. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71: 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  21. Riso P, Porrini M. 1996. Determination of carotenoids in vegetable foods and plasma. Internat J Vit Nutr Res 67: 47-54
  22. Mitsuda K, Mitsuda Y, Kohno M, Hiramatsu M, Mori A. 1990. The application of ESR spin-trapping technique to the evaluation of SOD-like activity of biological substances. Bull Chem Soc Jpn 63: 187-191 https://doi.org/10.1246/bcsj.63.187
  23. Sato M, Ramarathnam N, Suzuki Y, Ohkubo T, Takeuchi M, Ochi H. 1995. Varietal differences in the phenolic contents and superoxide radical scavenging potential of wine from different sources. J Agric Food Chem 44: 80-85 https://doi.org/10.1021/jf9502459
  24. Lissi E, Salim-Hanna M, Pascula C, Castillo MD. 1995. Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurement. Free Radic Biol Med 18: 153-158 https://doi.org/10.1016/0891-5849(94)00117-3
  25. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  26. Lim JA, Na YS, Baek SH. 2004. Antioxidant activity and nitrite scavenging ability of ethanol extract from Phyllostachys bambusoides. Korean J Food Sci Technol 36: 306-310
  27. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  28. Osawa T, Namiki M. 1981. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agric Biol Chem Tokyo 45: 735-739 https://doi.org/10.1271/bbb1961.45.735
  29. SAS Institute, Inc. 1996. SAS Institute Inc. SAS Campus Drive. Cary, NC, USA
  30. Bunea A, Andjelkovic M, Socaciu C, Bobis O, Neacsu M, Verhe R, Camp JV. 2008. Total and individual carotenoids and phenolic acids content in fresh, refrigerated and processed spinach (Spinacia oleracea L.). Food Chem 108: 649-656 https://doi.org/10.1016/j.foodchem.2007.11.056
  31. Kim MH, Lee NH, Lee MH, Kwon DJ, Choi UK. 2007. Antimicrobial activity of aqueous ethanol extracts of Penilla frutescens var. acuta leaf. Korean J Food Culture 22: 266-273
  32. Choi EM, Ding Y, Nguyen HT, Park SH, Kim YH. 2007. Antioxidant activity of Gomchi (Ligulara fischeri) leaves. Food Sci Biotechnol 16: 710-714
  33. Kim EK, Lee SJ, Lim BO, Jeon YJ, Song MD, Park TK, Lee KH, Kim BK, Lee SR, Moon SH, Jeon BT, Park PJ. 2008. Antioxidative and neuroprotective effects of enzymatic extracts from leaves of Perilla frutescens var. japonica. Food Sci Biotechnol 17: 279-286
  34. Beckman JS, Koppenol WH. 1996. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and ugly. Am J Physiol 217: 1421-1437 https://doi.org/10.1152/ajpcell.1996.271.5.C1424
  35. Lee YR, Hwang IG, Woo KS, Kim DJ, Hong JT, Jeong HS. 2007. Antioxidative activities of the ethyl acetate fraction from heated onion (Allium cepa). Food Sci Biotechnol 16: 1041-1045
  36. Kim HK, Cheon BS, Kim YH, Kim SY, Kim HP. 1999. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochem Pharmacol 58: 759-765 https://doi.org/10.1016/S0006-2952(99)00160-4
  37. Hong HD, Kang NG, Kim SS. 1998. Superoxide dismutase-like activity of apple juice mixed with some fruits and vegetable. Korean J Food Sci Technol 30: 1484-1487