Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Tyrosinase Inhibitory Effects of Paeonia suffruticosa Water Extract

목단피 물 추출물의 항산화 및 Tyrosinase 억제효과

  • You, Jin-Kyoun (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • Kim, Dae-Jung (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Seo, Dong-Joo (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Park, Jeong-Hae (Hwajin Cosmetic R&D Center) ;
  • Kim, Tae-Woo (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Choe, Myeon (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
  • 유진균 (강원대학교 식물생명공학) ;
  • 정미자 (강원대학교 BK21사업단(뉴트라슈티컬바이오)) ;
  • 김대중 (강원대학교 식물생명공학) ;
  • 서동주 (강원대학교 식물생명공학) ;
  • 박정해 (화진화장품 기술연구소) ;
  • 김태우 (강원대학교 식물생명공학) ;
  • 최면 (강원대학교 식물생명공학)
  • Published : 2009.03.31
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Abstract

Tyrosinase catalyzes melanin synthesis in skin melanocytes. The effects of Paeonia sufficinalis water extract (MDP) on antioxidant and tyrosinase activities have been studied in a cell-free system and mouse melanoma B16 cell. Radical scavenging activity of MDP was tested by DPPH assay and it showed high DPPH radical scavenging activity. The cellular tyrosinase activity was measured in mouse melanoma B16 cell by RT-PCR and enzyme activity. Treatment with MDP for 24 hr resulted in decreased tyrosinase mRNA level. Tyrosinase activity was decreased, compared with control, in cells exposed to MDP. Thus, Paeonia sufficinalis water extracts may be a candidate for cosmetic use.

Tyrosinase는 피부의 melanocytes에서 멜라닌 생성을 촉진한다. 목단피의 미백효과를 알아보기 위하여 목단피 물추출물의 항산화 능력과 tyrosinase 활성 억제에 미치는 영향을 세포 모델계와 a cell-free 모델계에서 알아보았다. MDP의 라디칼 소거작용은 DPPH assay로 알아보았고, MDP는 높은 DPPH 라디칼 소거작용을 보여주었다. 세포내 tyrosinase 활성은 RT-PCR, 효소활성을 마우스 B16 세포내에서 측정함으로써 알아보았다. 24시간 목단피 물 추출물을 투여한 세포내에서 tyrosinase mRNA와 효소활성이 무처리군과 비교하여 현저히 감소하였다. 이와 같은 결과들은 항산화력이 높고, tyrosinase를 효과적으로 억제할 수 있는 목단피 물 추출물을 이용하여 melanin 생합성을 억제할 수 있는 미백 화장품 개발에 활용할 수 있는 가능성을 제시하고 있다.

Keywords

References

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