Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Identification and molecular characterization of a rabbit hemorrhagic disease virus variant (KV0801) isolated in Korea

  • Yang, Dong-Kun (National Veterinary Research and Quarantine Service) ;
  • Kim, Byoung-Han (National Veterinary Research and Quarantine Service) ;
  • Lee, Kyung-Woo (National Veterinary Research and Quarantine Service) ;
  • Kim, Ji-Yeon (National Veterinary Research and Quarantine Service) ;
  • Kim, Hee-Jin (National Veterinary Research and Quarantine Service) ;
  • Choi, Sung-Suk (National Veterinary Research and Quarantine Service) ;
  • Chun, Ji-Eun (National Veterinary Research and Quarantine Service) ;
  • Son, Seong-Wan (National Veterinary Research and Quarantine Service)
  • Accepted : 2009.09.08
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Rabbit hemorrhagic disease (RHD) is caused by RHD virus (RHDV) and is one of the most fatal diseases of rabbits. Acute death of rabbits occurred in a farm located in the Gyeonggi province of South Korea. The virus was isolated and confirmed as RHDV based on reverse transcription polymerase chain reaction and hemagglutination assay (HA), and the isolate was designated as KV0801. The nucleotide sequence of the complete VP60 gene of KV0801 was determined and the corresponding amino acid sequence was deduced. Molecular analysis showed that the KV0801 isolate can be classified as a pandemic antigenic variant strain, RHDVa. The VP60 nucleotide sequence and deduced amino acid homology between KV0801 and other Korean isolate, RHF89, which was isolated in 1988, were 92.1 and 94.3%, respectively. The pathogenicity of the KV0801 isolate at an HA titer ranging from 16,384 to 0.16 HA units was evaluated in five-month-old SFP rabbits. The rabbits inoculated with KV0801 isolate containing more than 1.63 HA units died within six days of inoculation. These results suggest that a highly pathogenic RHDVa is circulating in the rabbit populations of Korea.

Keywords

References

  1. An SH, Kim BH, Lee JB, Song JY, Park BK, Kwon YB, Jung JS, Lee YS. Studies on picornavirus haemorrhagic fever (tentative name) in rabbit. I. Physico-chemical properties of the causative virus. Res Rept RDA 1988, 31, 55-61
  2. Capucci L, Fallacara F, Grazioli S, Lavazza A, Pacciarini ML, Brocchi E. A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant. Virus Res 1998, 58, 115-126 https://doi.org/10.1016/S0168-1702(98)00106-3
  3. Forrester NL, Abubakr MI, Abu Elzein EM, Al- Afaleq AI, Housawi FM, Moss SR, Turner SL, Gould EA. Phylogenetic analysis of rabbit haemorrhagic disease virus strains from the Arabian Peninsula: did RHDV emerge simultaneously in Europe and Asia? Virology 2006, 344, 277-282 https://doi.org/10.1016/j.virol.2005.10.006
  4. Gall A, Schirrmeier H. Persistence of rabbit haemorrhagic disease virus genome in vaccinatedrabbits after experimental infection. J Vet Med B Infect Dis Vet Public Health 2006, 53, 358-362 https://doi.org/10.1111/j.1439-0450.2006.00986.x
  5. Kerr PJ, Best SM. Myxoma virus in rabbits. Rev Sci Tech 1998, 17, 256-268 https://doi.org/10.20506/rst.17.1.1081
  6. Le Gall G, Arnauld C, Boilletot E, Morisse JP, Rasschaert D. Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995. J Gen Virol 1998, 79, 11-16 https://doi.org/10.1099/0022-1317-79-1-11
  7. Le Gall-Recule G, Zwingelstein F, Laurent S, de Boisseson C, Portejoie Y, Rasschaert D. Phylogenetic analysis of rabbit haemorrhagic disease virus in France between 1993 and 2000, and the characterisation of RHDV antigenic variants. Arch Virol 2003, 148, 65- 81 https://doi.org/10.1007/s00705-002-0908-1
  8. Liu G, Zhang Y, Ni Z, Yun T, Sheng Z, Liang H, Hua J, Li S, Du Q, Chen J. Recovery of infectious rabbit hemorrhagic disease virus from rabbits after direct inoculation with in vitro-transcribed RNA. J Virol 2006, 80, 6597-6602
  9. Liu SJ, Xue HP, Pu BQ, Quian NH. A new viral disease in rabbits. Anim Husb Vet Med 1984, 16, 253- 255
  10. Matiz K, Ursu K, Kecskemeti S, Bajmocy E, Kiss I. Phylogenetic analysis of rabbit haemorrhagic disease virus (RHDV) strains isolated between 1988 and 2003 in eastern Hungary. Arch Virol 2006, 151, 1659-1666 https://doi.org/10.1007/s00705-006-0730-2
  11. McIntosh MT, Behan SC, Mohamed FM, Lu Z, Moran KE, Burrage TG, Neilan JG, Ward GB, Botti G, Capucci L, Metwally SA. A pandemic strain of calicivirus threatens rabbit industries in the Americas. Virol J 2007, 4, 96
  12. Meyers G, Wirblich C, Thiel HJ, Thumfart JO. Rabbit hemorrhagic disease virus: genome organization and polyprotein processing of a calicivirus studied after transient expression of cDNA constructs. Virology 2000, 276, 349-363 https://doi.org/10.1006/viro.2000.0545
  13. Ministry of Agriculture and Forestry (MAF). Agricultural and Forestry Statistical Yearbook. p. 131, MAF, Seoul, 2007
  14. Moss SR, Turner SL, Trout RC, White PJ, Hudson PJ, Desai A, Armesto M, Forrester NL, Gould EA. Molecular epidemiology of rabbit haemorrhagic disease virus. J Gen Virol 2002, 83, 2461-2467 https://doi.org/10.1099/0022-1317-83-10-2461
  15. Office International des Epizooties (OIE). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 6th ed. pp. 947-961, OIE, Paris, 2008
  16. Park JH, Lee YS, Itakura C. Fibrin(ogen)-related antigens in rabbits experimentally infected with rabbit haemorrhagic disease virus. Res Vet Sci 1997, 63, 123- 127 https://doi.org/10.1016/S0034-5288(97)90004-7
  17. Park NY, Chong CJ, Kim JH, Cho SM, Cha YH, Jung BT, Kim DS, Yoon JB. An outbreak of viral haemorrhagic pneumonia (tentative name) of rabbits in Korea. J Korean Vet Med Assoc 1987, 23, 603-610
  18. Rodak L, Smid B, Valicek L, Vesely T, St panek J, Hampl J, Jurak E. Enzyme-linked immunosorbent assay of antibodies to rabbit haemorrhagic disease virus and determination of its major structural proteins. J Gen Virol 1990, 71, 1075-1080 https://doi.org/10.1099/0022-1317-71-5-1075
  19. Ruvoen-Clouet N, Blanchard D, Andre-Fontaine G, Song B, Ganiere JP. Detection of antibodies to rabbit haemorrhagic disease virus: an immunoblotting method using virus-coated human erythrocyte membranes. Zentralbl Veterinarmed B 1995, 42, 197-204
  20. Schirrmeier H, Reimann I, Kollner B, Granzow H. Pathogenic, antigenic and molecular properties of rabbit haemorrhagic disease virus (RHDV) isolated from vaccinated rabbits: detection and characterization of antigenic variants. Arch Virol 1999, 144, 719-735 https://doi.org/10.1007/s007050050538
  21. Teifke JP, Reimann I, Schirrmeier H. Subacute liver necrosis after experimental infection with rabbit haemorrhagic disease virus (RHDV). J Comp Pathol 2002, 126, 231-234 https://doi.org/10.1053/jcpa.2001.0534
  22. Tham KM, Barnes SM, Hunter SN. Polymerase chain reaction amplification and gene sequence analysis of a calicivirus from a feral rabbit. Virus Genes 1999, 18, 235-242 https://doi.org/10.1023/A:1008020303036
  23. Thumfart JO, Meyers G. Rabbit hemorrhagic disease virus: identification of a cleavage site in the viral polyprotein that is not processed by the known calicivirus protease. Virology 2002, 304, 352-363 https://doi.org/10.1006/viro.2002.1660
  24. Yang L, Wang F, Hu B, Xue J, Hu Y, Zhou B, Wang D, Xu W. Development of an RT-PCR for rabbit hemorrhagic disease virus (RHDV) and the epidemiology of RHDV in three eastern provinces of China. J Virol Methods 2008, 151, 24-29 https://doi.org/10.1016/j.jviromet.2008.04.003