Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis

  • Lee, Ji Hye (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Keun Jung (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Seon A (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Li, Xiaoxia (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Eun Young (College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oh, Hyun Ju (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong Chun (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Hye Jin (Haemaru Small Animal Referral Hospital) ;
  • Park, Byung Kwon (College of Industrial Science, Kongju National University) ;
  • Kim, Min Kyu (College of Agriculture and Life Sciences, Chungnam National University)
  • Accepted : 2009.12.02
  • Published : 2009.12.31
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Abstract

This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with $1.3{\times}$ $10^8$ spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.

Keywords

References

  1. Andersen K. Insemination with frozen dog semen based on a new insemination technique. Zuchthygiene 1975, 10, 1-4 https://doi.org/10.1111/j.1439-0531.1975.tb00275.xAboutDOI
  2. Cremonesi F, Salamon L, Groppetti D, Pecile A. Results of a single transcervical endoscopic insemination using frozen semen in the bitch. Vet Res Commun 2005, 29 (Suppl 2), 187-189 https://doi.org/10.1007/s11259-005-0039-8
  3. Farstad W. Bitch fertility after natural mating and after artificial insemination with fresh or frozen semen. J Small Anim Pract 1984, 25, 561-565 https://doi.org/10.1111/j.1748-5827.1984.tb03429.x
  4. Farstad W, Berg KA. Factors influencing the success rate of artificial insemination with frozen semen in the dog. J Reprod Fertil Suppl 1989, 39, 289-292
  5. Ferguson JM, Renton JP, Farstad W, Douglas TA. Insemination of beagle bitches with frozen semen. J Reprod Fertil Suppl 1989, 39, 293-298
  6. Fredholm M, Winter$\phi$ AK. Efficient resolution of parentage in dogs by amplification of microsatellites. Anim Genet 1996, 27, 19-23
  7. Georges M, Hilbert P, Lequarre AS, Leclerc V, Hanset R, Vassart G. Use of DNA bar codes to resolve a canine paternity dispute. J Am Vet Med Assoc 1988, 193, 1095-1098
  8. Johnston SD, Root Kustritz MV, Olson PNS. Canine and Feline Theriogenology. 1st ed. pp. 41-65, Saunders, Philadelphia, 2001
  9. Lee BC, Kim MK, Jang G, Oh HJ, Yuda F, Kim HJ, Hossein MS, Kim JJ, Kang SK, Schatten G, Hwang WS. Dogs cloned from adult somatic cells. Nature 2005, 436, 641 https://doi.org/10.1038/436641a
  10. Linde-Forsberg C. Achieving canine pregnancy by using frozen or chilled extended semen. Vet Clin North Am Small Anim Pract 1991, 21, 467-485 https://doi.org/10.1016/S0195-5616(91)50054-1
  11. Linde-Forsberg C. Artificial insemination with fresh, chilled extended, and frozen-thawed semen in the dog. Semin Vet Med Surg (Small Anim) 1995, 10, 48-58
  12. Linde-Forsberg C, Forsberg M. Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen. J Reprod Fertil Suppl 1989, 39, 299-310
  13. Linde-Forsberg C, Forsberg M. Results of 527 controlled artificial inseminations in dogs. J Reprod Fertil Suppl 1993, 47, 313-323
  14. Linde-Forsberg C, Str$\"{o}$m Holst B, Govette G. Comparison of fertility data from vaginal vs intrauterine insemination of frozen-thawed dog semen:a retrospective study. Theriogenology 1999, 52, 11-23 https://doi.org/10.1016/S0093-691X(99)00106-5
  15. N$\"{o}$thling JO, Gerstenberg C, Volkmann DH. Success with intravaginal insemination of frozenthawed dog semen - a retrospective study. J S Afr Vet Assoc 1995, 66, 49-55
  16. N$\"{o}$thling JO, Shuttleworth R. The effect of straw size, freezing rate and thawing rate upon post-thaw quality of dog semen. Theriogenology 2005, 63, 1469-1480 https://doi.org/10.1016/j.theriogenology.2004.07.012
  17. Olar TT, Bowen RA, Pickett BW. Influence of extender, cryoperservative and seminal processing procedures on postthaw motility of canine spermatozoa frozen in straws. Theriogenology 1989, 31, 451-461 https://doi.org/10.1016/0093-691X(89)90550-5
  18. Parker HG, Kruglyak L, Ostrander EA. Molecular genetics: DNA analysis of a putative dog clone. Nature 2006, 440, E1-2 https://doi.org/10.1038/nature04685
  19. Rijsselaere T, Van Soom A, Van Cruchten S, Coryn M, Gortz K, Maes D, de Kruif A. Sperm distribution in the genital tract of the bitch following artificial insemination in relation to the time of ovulation. Reproduction 2004, 128, 801-811 https://doi.org/10.1530/rep.1.00273
  20. Root Kustritz MV. Clinical management of pregnancy in cats. Theriogenology 2006, 66, 145-150 https://doi.org/10.1016/j.theriogenology.2006.03.018
  21. Rota A, Iguer-Ouada M, Verstegen J, Linde- Forsberg C. Fertility after vaginal or uterine deposition of dog semen frozen in a tris extender with or without Equex STM paste. Theriogenology 1999, 51, 1045-1058 https://doi.org/10.1016/S0093-691X(99)80010-7
  22. Seager SWJ. Successful pregnancies utilizing frozen dog semen. AI Digest 1969, 17, 6-16
  23. Silva LD, Onclin K, Lejeune B, Verstegen JP. Comparisons of intravaginal and intrauterine insemination of bitches with fresh or frozen semen. Vet Rec 1996, 138, 154-157 https://doi.org/10.1136/vr.138.7.154
  24. Thomassen R, Farstad W, Krogenaes A, Fougner JA, Berg KA. Artificial insemination with frozen semen in dogs: a retrospective study. J Reprod Fertil Suppl 2001, 57, 341-346
  25. Tsumagari S, Ichikawa Y, Toriumi H, Ishihama K, Morita M, Kanamaki M, Takeishi M. Optimal timing for canine artificial insemination with frozen semen and parentage testing by microsatellite markers in superfecundency. J Vet Med Sci 2003, 65, 1003-1005 https://doi.org/10.1292/jvms.65.1003
  26. Tsutsui T, Ejima H. Experimental induction of superfecundation in the dog. Nippon Juigaku Zasshi 1988, 50, 581-583 https://doi.org/10.1292/jvms1939.50.581
  27. Tsutsui T, Hase M, Tanaka A, Fujimura N, Hori T, Kawakami E. Intrauterine and intravaginal insemination with frozen canine semen using an extender consisting of orvus ES paste-supplemented egg yolk tris-fructose citrate. J Vet Med Sci 2000, 62, 603-606 https://doi.org/10.1292/jvms.62.603
  28. Tsutsui T, Hori T, Yamada A, Kirihara N, Kawakami E. Intratubal insemination with fresh semen in dogs. J Vet Med Sci 2003, 65, 659-661 https://doi.org/10.1292/jvms.65.659
  29. Tsutsui T, Shimizu T, Ohara N, Shiba Y, Hironaka T, Orima H, Ogasa A. Relationship between the number of sperms and the rate of implantation in bitches inseminated into unilateral uterine horn. Nippon Juigaku Zasshi 1989, 51, 257-263 https://doi.org/10.1292/jvms1939.51.257
  30. Tsutsui T, Tezuka T, Mikasa Y, Sugisawa H, Kirihara N, Hori T, Kawakami E. Artificial insemination with canine semen stored at a low temperature. J Vet Med Sci 2003, 65, 307-312 https://doi.org/10.1292/jvms.65.307
  31. Tsutsui T, Tezuka T, Shimizu T, Murao I, Kawakami E, Ogasa A. Artificial insemination with fresh semen in beagle bitches. Nippon Juigaku Zasshi 1988, 50, 193-198 https://doi.org/10.1292/jvms1939.50.193
  32. Wildt DE, Chan SY, Seager SW, Chakraborty PK. Ovarian activity, circulating hormones, and sexual behavior in the cat. I. Relationships during the coitusinduced luteal phase and the estrous period without mating. Biol Reprod 1981, 25, 15-28 https://doi.org/10.1095/biolreprod25.1.15
  33. Wilson MS. Non-surgical intrauterine artificial insemination in bitches using frozen semen. J Reprod Fertil Suppl 1993, 47, 307-311