Korean Chemical Engineering Research

  • 제47권5호
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  • Pages.538-545
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  • 2009
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
권호 표지

원자간력 현미경을 이용한 단일세포 조작 및 고효율 유전자 도입기술

Atomic Force Microscopy(AFM) based Single Cell Manipulation and High Efficient Gene Delivery Technology

  • 한성웅 (일본이화학연구소 세포시뮬레이션팀) ;
  • ;
  • ;
  • 김우식 (경희대학교 화학공학과) ;
  • 김종민 (동아대학교 화학공학과) ;
  • 장상목 (동아대학교 화학공학과)
  • Han, Sung-Woong (Computational Cell Biomechanics Team, VCAD System Research Program) ;
  • Nakamura, Chikashi (Research Institute for Cell Engineering, AIST) ;
  • Miyake, Jun (Research Institute for Cell Engineering, AIST) ;
  • Kim, Woo-Sik (Department of Chemical Engineering, Kyung Hee University) ;
  • Kim, Jong-Min (Department of Chemical Engineering, Dong A University) ;
  • Chang, Sang-Mok (Department of Chemical Engineering, Dong A University)
  • 투고 : 2009.07.08
  • 심사 : 2009.08.03
  • 발행 : 2009.10.31
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초록

본 총설에서는 주사형프로브현미경의 원리와 응용에 관하여 간략히 설명하고 최근 본 그룹에 의하여 활발하게 연구되고 있는 나노탐침과 AFM(원자간력현미경 atomic force microscopy)을 이용한 저침습성(low-invasive) 단일세포 조작기술과 고효율 유전자 도입기술을 소개하고자 한다. 시판 AFM 탐침을 침상구조로 가공한 나노탐침과 AFM을 이용하였을 경우, 탐침의 세포삽입의 성공여부를 force-distance curve 상의 척력소실의 유무로 판단할 수 있다. 침상 나노탐침을 사용하면 대부분의 세포에서 80~90%의 고효율 세포삽입이 가능하여 마이크로인젝션용 미세관을 이용하는 경우보다 세포삽입효율이 높았다. 또한 나노탐침의 직경이 400 nm 이하의 경우에는 세포 종류에 관계없이 장시간 나노탐침의 삽입에도 세포활성에 큰 영향이 없었다. 침상나노탐침을 이용하여 DNA를 도입하였을 경우에도 기존의 DNA 도입방법과 비교하여 높은 도입효율과 유전자 발현율로 DNA를 도입할 수 있는 가능성을 확인하였다.

The principle and application of a scanning probe microscopy(SPM) are reviewed briefly, and a low-invasive single cell manipulation and a gene delivery technique using an etched atomic force microscopy(AFM) probe tip, which we call a nanoneedle, are explained in detail. The nanoneedle insertion into a cell can be judged by a sudden drop of force in a force-distance curve. The probabilities of nanoneedle insertion into cells were 80~90%, which were higher than those of typical microinjection capillaries. When the diameter of the nanoneedle was smaller than 400 nm, the nanoneedle insertion into a cell over 1 hour had almost no influence on the cell viability. A highly efficient gene delivery and a high ratio of expressed gene per delivered DNA compared the conventional major nonviral gene delivery methods could be achieved using the gene modified nanoneedle.

키워드

과제정보

연구 과제 주관 기관 : 동아대학교

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