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Activation of apoptotic protein in U937 cells by a component of turmeric oil

  • Lee, Yong-Kyu (Department of Food and Biotechnology, Dongseo University)
  • Published : 2009.02.28

Abstract

Aromatic (ar)-turmerone from turmeric oil displays anti-tumorigenesis activity that includes inhibited cell proliferation. This study investigated ar-turmerone-mediated apoptotic protein activation in human lymphoma U937 cells. Ar-turmerone treatment inhibited U937 cell viability in a concentration-dependent fashion, with inhibition exceeding 84%. Moreover, the treatment produced nucleosomal DNA fragmentation and the percentage of sub-diploid cells increased in a concentration-dependent manner; both are hallmarks of apoptosis. The apoptotic effect of ar-turmerone was associated with the induction of Bax and p53 proteins, rather than Bcl-2 and p21. Activation of mitochondrial cytochrome c and caspase-3 demonstrated that the activation of caspases accompanied the apoptotic effect of ar-turmerone, which mediated cell death. These results suggest that the apoptotic effect of ar-turmerone on U937 cells may involve caspase-3 activation through the induction of Bax and p53, rather than Bcl-2 and p21.

Keywords

References

  1. Wyllie, A.H., Kerr, J.F. and Currie, A.R. (1980) Cell death: the significance of apoptosis. Int. Rev. Cytol. 68, 251-306 https://doi.org/10.1016/S0074-7696(08)62312-8
  2. Liu, X., Kim, C.N., Yang, J., Jemmerson, R. and Wang, X. (1996) Induction of apoptotic program in cell-free extract: requirement for dATP and cytochrome c. Cell 80, 147-157
  3. Li, P., Nijhawan, D., Budihardjo, I., Srinivasula, S.M., Ahmad, M., Alnemri, E.S. and Wang, X. (1997) Cytochrome c and dATP-dependent formation of Apaf-1/caspase complex initiates an apoptotic protease cascade. Cell 91, 479-489 https://doi.org/10.1016/S0092-8674(00)80434-1
  4. Stennicke, H.R. and Salvesen, G.S. (1998) Properties of the caspases. Biochem. Biophysics. Acta. 1387, 17-31 https://doi.org/10.1016/S0167-4838(98)00133-2
  5. Miyashita, T., Krajewski, S., Krajewska, M., Wang, H.G.,Lin, H.K., Liebermann, D.A., Hoffman, B. and Reed, J.C. (1994) Tumor suppressor p53 is a regulator of Bcl-2 and Bax gene expression in vitro and in vivo. Oncogene 9, 1799-1805
  6. Lowe, S.W., Ruley, H.E., Jacks, T. and Housman, D.E. (1993) P53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 74, 957-967 https://doi.org/10.1016/0092-8674(93)90719-7
  7. El-Deiry, W.S., Tokino, T., Velculescu, V.E., Levy, D.B., Parsons, R., Trent, J.M., Lin, D., Mercer, W.E., Kinzler, K.W. and Vogelstein, B. (1993) WAF1, a potential mediator of p53 tumor suppression. Cell 75, 817-825 https://doi.org/10.1016/0092-8674(93)90500-P
  8. Tsujimoto, Y. (1998) Role of Bcl-2 family proteins in apoptosomes or mitochondria? Genes Cells 3, 697-707 https://doi.org/10.1046/j.1365-2443.1998.00223.x
  9. Rosse, T., Olivier ,R., Monney, L., Rager M., Conus, S., Fellay, I., Jansen, B. and Borner, C. (1998) Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c. Nature 391, 496-499 https://doi.org/10.1038/35160
  10. Finucanne, D.M., Bossy-Wetzel, E., Waterhouse, N.J., Cotter, T.G. and Green, D.R. (1999) Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-XL. J. Biol. Chem. 274, 2225-2233 https://doi.org/10.1074/jbc.274.4.2225
  11. Roth, G.N., Chandra, A. and Nair, M.G. (1998) Novel bioactivities of Curcuma longa constituents. J. Nat. Prod. 61, 542-545 https://doi.org/10.1021/np970459f
  12. Manzan, A.C., Toniolo, F.S., Bredow, E. and Povj, N.P. (2003) Extraction of essential oil and pigments from Curcuma longa by steam distillation and extraction with volatile solvents. J. Agric. Food Chem. 51, 6802-6807 https://doi.org/10.1021/jf030161x
  13. Ji, M.G., Choi, J.S., Lee, J.N. and Lee, Y.K. (2004) Induction of apoptosis by ar-turmerone on various cell lines. Int. J. Mol. Med. 14, 253-256
  14. Hongo, T., Fujii, Y. and Igarashi, Y. (1990) An in vitro chemosensitivity test for the screening of anti-cancer drugs in childhood leukemia. Cancer 65, 1263-1272 https://doi.org/10.1002/1097-0142(19900315)65:6<1263::AID-CNCR2820650602>3.0.CO;2-S
  15. Mossman, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  16. Kluck, R.M., Bossy-Wetzel, E., Green, D.R. and Newmeyer, D.D. (1997) The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science 275, 1132-1136 https://doi.org/10.1126/science.275.5303.1132
  17. Kroemer, G., Dallaporta, B. and Resche-Rigon, M. (1998) The mitochondrial death/life regulator in apoptosis and necrosis. Annu. Rev. Physiol. 60, 619-642 https://doi.org/10.1146/annurev.physiol.60.1.619
  18. Hsu, Y.T., Wolter K.G. and Youle, R.J. (1997) Cytosol to membrane redistribution of Bax and Bcl-X (L) during apoptosis. Proc. Natl. Acad. Sci. U.S.A. 94, 3668-3672 https://doi.org/10.1073/pnas.94.8.3668
  19. Chao, D.T. and Korsmeyer, S.J. (1998) Bcl-2 family: regulators of cell death. Annu. Rev. Immunol. 16, 395-419 https://doi.org/10.1146/annurev.immunol.16.1.395
  20. Thornberry, N.A., Bull, H.G., Calaycay, J.R., Chapman, K.T., Howard, A.D., Kostura, M.J., Miller, D.K., Molineaux, S.M., Weidner, J.R. and Aunius, J. (1992) A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes. Nature 356, 768-774 https://doi.org/10.1038/356768a0
  21. Yuan, J., Shaham, S., Ledoux, S., Ellis, H.M. and Horvitz, H.R. (1993) The C. Elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme. Cell 75, 641-652 https://doi.org/10.1016/0092-8674(93)90485-9
  22. Sentman, C.L., Shutter, J.R., Hockenbery, D., Kanagawa, O. and Korsmeyer, S.J. (1991) Bcl-2 inhibits multiple forms of apoptosis but not negative selection in thymocyte. Cell 67, 879-888 https://doi.org/10.1016/0092-8674(91)90361-2
  23. Decaudin, D., Marzo, I., Brenner, C. and Kroemer, G. (1998) Mitochondria in chemotherapy-induced apoptosis: a prospective novel target of cancer therapy. Int. J. Oncol. 12, 141-152
  24. Earnshaw, W.C., Martins, L.M. and Kaufmann, S.H. (1996) Mammalian caspases: structure, activation, substrates and functions during apoptosis. Annu. Rev. Biochem. 6, 383-424
  25. Hong, C.H., Kim, Y.L. and Lee, S.K. (2001) Sesquiterpenoids from the rhizome of Curcuma zedoaria. Arch. Pharm. Res. 24, 424-426 https://doi.org/10.1007/BF02975188
  26. Mohammad, A.M., Razieh, Y. and Mohammad, H.S.(2005) The cytotoxic and anti-proliferative effects of 30hydrogenkwadaphin in K562 and Jurkat cells is reduced by guanosine. J. Biochem. Mol. Biol. 38, 391-398 https://doi.org/10.5483/BMBRep.2005.38.4.391
  27. Bhalla, R.C., Toth, K.F., Bhatty, R.A., Thompson, L.P. and Sharma, R.V. (1997) Estrogen reduces proliferation and agonist- induced calcium increase in coronary artery smooth muscle cells. Am. J. Physiol. 272, H1996-2003

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