Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Quantitative Trait Loci Affecting Rous Sarcoma Virus Induced Tumor Regression Trait in F2 Intercross Chickens

  • Uemoto, Y. (National Livestock Breeding Center) ;
  • Saburi, J. (National Livestock Breeding Center) ;
  • Sato, S. (National Livestock Breeding Center) ;
  • Odawara, S. (National Livestock Breeding Center) ;
  • Ohtake, T. (National Livestock Breeding Center) ;
  • Yamamoto, R. (National Livestock Breeding Center) ;
  • Miyata, T. (National Livestock Breeding Center) ;
  • Suzuki, K. (STAFF Institute) ;
  • Yamashita, H. (Tokai University) ;
  • Irina, C. (National Institute of Livestock and Grassland Science) ;
  • Plastow, G. (University of Alberta) ;
  • Mitsuhashi, T. (National Institute of Livestock and Grassland Science) ;
  • Kobayashi, E. (National Livestock Breeding Center)
  • Received : 2009.02.24
  • Accepted : 2009.05.18
  • Published : 2009.10.01
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Abstract

We performed a genome-wide linkage and quantitative trait locus (QTL) analysis to confirm the existence of QTL affecting Rous Sarcoma Virus (RSV) induced tumor regression, and to estimate their effects on phenotypic variance in an F2 resource population. The F2 population comprised 158 chickens obtained by crossing tumor regressive White Leghorn (WL) and tumor progressive Rhode Island Red (RIR) lines was measured for tumor formation after RSV inoculation. Forty-three tumor progressive and 28 tumor regressive chickens were then used for genome-wide linkage and QTL analysis using a total of 186 microsatellite markers. Microsatellite markers were mapped on 20 autosomal chromosomes. A significant QTL was detected with marker LEI0258 located within the MHC B region on chromosome 16. This QTL had the highest F ratio (9.8) and accounted for 20.1% of the phenotypic variation. Suggestive QTL were also detected on chromosomes 4, 7 and 10. The QTL on chromosome 4 were detected at the 1% chromosome-wide level explaining 17.5% of the phenotypic variation, and the QTLs on chromosome 7 and 10 were detected at the 5% chromosome-wide level and explained 11.1% and 10.5% of the phenotypic variation, respectively. These results indicate that the QTLs in the non-MHC regions play a significant role in RSV-induced tumor regression. The present study constitutes one of the first preliminary reports in domestic chickens for QTLs affecting RSV-induced tumor regression outside the MHC region.

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References

  1. Abasht, B., J. C. Dekkers and S. J. Lamont. 2006, Review of quantitative trait loci identified in the chicken, Poult. Sci. 85: 2079-2096
  2. Brown, D. W., W. M. Collins, R. M. Zsigray and W. E. Briles. 1984, A non-MHC genetic influence on response to Rous sarcoma virus-induced tumors in chickens, Avian Dis. 28:884-899 https://doi.org/10.2307/1590265
  3. Bacon, L. D., R. L. Witter, L. B. Crittenden, A. Fadly and J. Motta.1981, B-haplotype influence on Marek's disease, Rous sarcoma, and lymphoid leukosis virus-induced tumors in chickens. Poult. Sci. 60:1132-1139 https://doi.org/10.3382/ps.0601132
  4. Calnek, B. W., D. A. Higgins and J. Fabricant. 1975, Rous sarcoma regression in chickens resistant or susceptible to Marek's disease, Avian Dis. 19:473-482 https://doi.org/10.2307/1589072
  5. Carte, I. F., J. H. Smith, C. R. Weston and T. F. Savage. 1972, immunogenetics and regression of RSV (RAV-1) wing web tumors in chickens, Poult. Sci. 51:1792 (Abstr)
  6. Cole, R. K. 1968, Studies on genetic resistance to Marek's disease, Avian Dis. 12:9-28 https://doi.org/10.2307/1588081
  7. Collins, W. M., W. E. Briles, R. M. Zsigray, W. R. Dunlop, A. C. Corbett, K. K. Clark, J. L. Marks and T. P. MacGrail. 1977, The B locus (MHC) in the chicken: Association with the fate of RSV-induced tumors, Immunogenetics 5:333-343 https://doi.org/10.1007/BF01570490
  8. Bates, P., L. Rong, H. E. Varmus, J. A. T. Young and L. B. Crittenden. 1998, Genetic mapping of the cloned subgroup A avian sarcoma and leukosis virus receptor gene to the TVA locus, J. Virol. 72:2505-2508
  9. Elleder, D., D. C. Melder, K. Trejbalova, J. Svoboda and M. J. Federspiel. 2004, Two different molecular defects in the tva receptor gene explain the resistance of two tvar lines of chickens to infection by subgroup A avian sarcoma and leukosis viruses, J. Virol. 78:13489-13500 https://doi.org/10.1128/JVI.78.24.13489-13500.2004
  10. Fulton, J., H. R. Juul-Madsen, C. M. Ashwell, A. A. McCarron, J. A. Arthur, N. P. O'Sullivan and R. L. Taylor. 2006, Molecular genotype identification of the gallus gallus major histocompatibility complex, Immunogenetics 58:407-421 https://doi.org/10.1007/s00251-006-0119-0
  11. Gelman, I. H. and H. Hanafusa. 1993, src-Specific immune regression of Rous sarcoma virus-induced tumors, Cancer Res.15:915-920
  12. Green, P., K. Falls and S. Crooks. 1990, Documentation for CRIMAP. Version 2.4. Washington University School of Medicine, ST. Louis, MO
  13. Haley, C. S., S. A. Knott and J. M. Elsen. 1994, Mapping quantitative trait loci in crosses between outbred lines using least squares, Genetics 136:1195-1207
  14. Hirooka, H., D. J. de Koning, J. A. van Arendonk, B. Harlizius, P. N. de Groot and H. Bovenhuis. 2002, Genome scan reveals new coat color loci in exotic pig cross. J. Hered. 93:1-8 https://doi.org/10.1093/jhered/93.1.1
  15. Kaufman, J. and K. Venugopal. 1998, The importance of MHC for Rous sarcoma virus and Marek's disease virus - some Payneful considerations, Avian Pathol. 27:82-87 https://doi.org/10.1080/03079459808419297
  16. Knap, P. W. and S. C. Bishop. 2000, Relationships between genetic change and infectious disease in domestic livestock. In: The challenge of genetic change in animal production (Ed. W. G. Hill, S. C. Bishop, B. McGuirk, J. C. McKay, G. Simm and A. J. Webb) British Society of Animal Science, Edinburgh, Occasional publication no. 27, pp. 65-80
  17. Knott, S. A., L. Marklund, C. S. Haley, K. Andersson, W. Davies, H. Ellegren, M. Fredholm, I. Hansson, B. Hoyheim, K. Lundstrom, M. Moller and L. Andersson. 1998, Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and Large White pigs, Genetics 149:1069-1080 https://doi.org/10.1590/S1415-47572008000300013
  18. Lamont, S. J. 1998, Impact of genetics on disease resistance, Poult. Sci. 77:1111-1118
  19. McKay, J. C., N. F. Barton, A. N. M. Koerhuis and J. McAdam. 2000, The challenge of genetic change in the broiler chicken. In: The challenge of genetic change in animal production (Ed. W. G. Hill, S. C. Bishop, B. McGuirk, J. C. McKay, G. Simm and A. J. Webb) British Society of Animal Science, Edinburgh, Occasional publication no. 27, pp. 1-7
  20. Okada, I., Y. Yamada, M. Akiyama, I. Nishimura and N. Kano. 1977, Changes in polymorphic gene frequencies in strains of chickens selected for resistance to marek's disease, Br. Poult. Sci. 18:237-246 https://doi.org/10.1080/00071667708416359
  21. Praharaj, N., C. Beaumont, G. Dambrine, D. Soubieux, L. Merat, D. Bouret, G. Luneau, J. M. Alletru, M. H. Pinard-Van der Laan, P. Thoraval and S. Mignon-Grasteau. 2004, Genetic analysis of the growth curve of rous sarcoma virus-induced tumors in chickens, Poult. Sci. 83:1479-1488
  22. Rous, P. 1911, A sarcoma of the fowl transmissible by an agent seperable from the tumor cell, J. Exp. Med. 13:397-411 https://doi.org/10.1084/jem.13.4.397
  23. Seaton, G., C. S. Haley, S. A. Knott, M. Kearsey and P. M. Visscher. 2002, QTL EXPRESS: mapping quantitative trait loci in simple and complex pedigrees, Bioinformatics 18:339-340 https://doi.org/10.1093/bioinformatics/18.2.339
  24. Svoboda, J., J. Plachy, J. Hejnar, I. Karakoz, R. V. Cuntaka and J. Geryk. 1992, Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens, Immunogenetics 35:309-315
  25. Taylor, R. L. 2004, Major histocompatibility (B) complex control of responses against Rous sarcomas, Poult. Sci. 83:638-649
  26. Vallejo, R. L., L. D. Bacon, H. C. Liu, R. L. Witter, M. A. M. Groenen, J. Hillel and H. H. Cheng. 1998, Genetic mapping of Marek's disease virus induced tumors in F2 intercross chickens, Genetics 148:349-360
  27. Visscher, P. M., C. S. Haley and S. A. Knott. 1996, Mapping QTL for binary traits in backcross and F2 populations, Genet. Res.68:55-63 https://doi.org/10.1017/S0016672300033887
  28. Yonash, N., L. D. Bacon, R. L. Witter and H. H. Cheng. 1999, High resolution mapping and identification of new quantitative trait loci (QTL) affecting susceptibility to Marek's disease, Anim. Genet. 30:126-135 https://doi.org/10.1046/j.1365-2052.1999.00457.x

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