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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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β-catenin protein utilized by Tumour necrosis factor-α in porcine preadipocytes to suppress differentiation

  • Luo, Xiao (Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University) ;
  • Li, Hui-Xia (Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University) ;
  • Liu, Rong-Xin (Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University) ;
  • Wu, Zong-Song (Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University) ;
  • Yang, Ying-Juan (Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University) ;
  • Yang, Gong-She (Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A & F University)
  • Published : 2009.06.30
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Abstract

The Wnt/$\beta$-catenin signaling pathway alters adipocyte differentiation by inhibiting adipogenic gene expression. $\beta$-catenin plays a central role in the Wnt/$\beta$-catenin signaling pathway. In this study, we revealed that tumour necrosis factor-$\alpha$ (TNF-$\alpha$), a potential negative regulator of adipocyte differentiation, inhibits porcine adipogenesis through activation of the Wnt/$\beta$-catenin signaling pathway. Under the optimal concentration of TNF-$\alpha$, the intracellular $\beta$-catenin protein was stabilized. Thus, the intracellular lipid accumulation of porcine preadipocyte was suppressed and the expression of important adipocyte marker genes, including peroxisome proliferator-activated receptor-$\gamma$ (PPAR$\gamma$) and CCAAT/enhancer binding protein-$\alpha$ (C/EBP$\alpha$), were inhibited. However, a loss of $\beta$-catenin in porcine preadipocytes enhanced the adipogenic differentiation and attenuated TNF-$\alpha$ induced anti-adipogenesis. Taken together, this study indicated that TNF-$\alpha$ inhibits adipogenesis through stabilization of $\beta$-catenin protein in porcine preadipocytes.

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