Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Urinary Catecholamine and Cortisol Responses of Japanese Shorthorn Cows to Social Isolation

  • Higashiyama, Yumi (National Agricultural Research Center for Tohoku Region) ;
  • Nashiki, M. (National Institute of Livestock and Grassland Science) ;
  • Narita, H. (Sapporo)
  • Received : 2009.02.12
  • Accepted : 2009.06.18
  • Published : 2009.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the use of urinary catecholamines to monitor changes in the activity of the sympathetic nervous system and to determine the relationship of urinary cortisol and catecholamines in Japanese Shorthorn cows in response to social isolation. One cow was isolated from its group, which consisted of 14 cows (457 to 756 kg BW, 2 to 12 years old), for three days. The isolated cow was in contact with the other cows visually only at meal times. This isolation was repeated for 6 cows. Spontaneously voided urine samples were collected from the experimental animals once a day, before the treatment and on days 1, 2, and 3. Urinary cortisol and adrenaline levels were significantly increased compared with pre-isolation levels on the first day, and then declined to the basal levels during the next two days. Urinary noradrenaline levels changed in the same way as cortisol and adrenaline levels, but the difference was not significant. Urinary cortisol levels tended to be correlated with those of urinary adrenaline, but not noradrenaline. This study suggests that the urinary adrenaline levels can be a non-invasive indicator of stress and that the change of urinary adrenaline is similar to that of urinary cortisol.

Keywords

References

  1. Apple, J. K., M. E. Dikeman, J. E. Minton, R. M. McMurphy, M.R. Fedde, D. E. Leith and J. A. Unruh. 1995, Effects of restraint and isolation stress and epidural blockade on endocrine and blood metabolite status, muscle glycogen metabolism, and incidence of dark-cutting longissimus muscle of sheep, J. Anim. Sci. 73:2295-2307
  2. Boissy, A. and P. Le Neindre. 1997, Behavioral, cardiac and cortisol responses to brief peer separation and reunion in cattle, Physiol. Behav 61:693-699 https://doi.org/10.1016/S0031-9384(96)00521-5
  3. Dehnhard, M. 2007, Characterisation of the sympathetic nervous system of Asian (Elephas maximus) and African (Loxodonta Africana) elephants based on urinary catecholamine analyses, Gen. Comp. Endocrinol 151:274-284 https://doi.org/10.1016/j.ygcen.2007.01.001
  4. Foury, A., N. Devillers, M.-P. Sanchez, H. Griffon, P. Le Roy and P. Mormède. 2005, Stress hormones, carcass composition and meat quality in Large White×Duroc pigs, Meat Sci 69:703-707 https://doi.org/10.1016/j.meatsci.2004.11.002
  5. Foury, A., N. A. Geverink, M. Gil, M. Gispert, M. Hort$\acute{o}$s, M. Font I Furnols, D. Carrion, S. C. Blott, G. S. Plastow and P. Morm$\grave{e}$de. 2007, Stress neuroendocrine profiles in five pig breeding lines and the relationship with carcass composition. Animal 1:973-982 https://doi.org/10.1017/S1751731107000249
  6. Hay, M., M.-C. Meunier-Salaun, F. Brulaud, M. Monnier and P. Mormede. 2000, Assessment of hypothalamic-pituitary-adrenal axis and sympathetic nervous system activity in pregnant sows through the measurement of glucocorticoids andcatecholamines in urine, J. Anim. Sci 78:420-428
  7. Higasihyama, Y., H. Narita, M. Nashiki, M. Higashiyama and T. Kanno. 2005, Urinary cortisol levels in Japanese Shorthorn cattle before and after the start of a grazing season, Asian-Aust.J. Anim. Sci 18:1430-1434
  8. Higashiyama, Y., M. Nashiki, H. Narita and M. Kawasaki. 2007, A brief report on effects of transfer from outdoor grazing to indoor tethering and back on urinary cortisol and behaviour in dairy cattle, Appl. Anim. Behav. Sci 102:119-123 https://doi.org/10.1016/j.applanim.2006.03.007
  9. Lowe, T. E., C. E. Devine, R. W. Wells and L. L. Lynch. 2004, The relationship between postmortem urinary catecholamines, meat ultimate pH, and shear force in bulls and cows, Meat Sci 67:251-260 https://doi.org/10.1016/j.meatsci.2003.10.013
  10. Miller, M. W., N. T. Hobbs and M. C. Sousa. 1991, Detecting stress responses in Rocky Mountain bighorn sheep (Ovis canadensis canadensis): reliability of cortisol concentrations in urine and feces, Can. J. Zool 69:15-24 https://doi.org/10.1139/z91-003
  11. Minton, J. E. 1994, Function of the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system in models of acute stress in domestic farm animals, J. Anim. Sci 72:1891-1898
  12. Munksgaard, L. and H. B. Simonsen. 1996, Behavioral and pituitary adrenal-axis responses of dairy cows to social isolation and deprivation of lying down, J. Anim. Sci 74:769-778 https://doi.org/10.3168/jds.2007-0494
  13. Odore, R., A. D'Angelo, P. Badino, C. Bellino, S. Pagliasso and G. Re. 2004, Road transportation affects blood hormone levels and lymphocyte glucocorticoid and $\beta$-adrenergic receptor concentrations in calves, Vet. J 168:297-303 https://doi.org/10.1016/j.tvjl.2003.09.008
  14. Rushen, J., A. Boissy, E. M. C. Terlouw and A. M. B. de Passille. 1999, Opioid peptides and behavioral and physiological responses of dairy cows to social isolation in unfamiliar surroundings, J. Anim. Sci77:2918-2924
  15. Scheffe, H. 1959, The analysis of variance, John Wiley & Sons, Inc., NY
  16. Smulders, D., G. Verbeke, P. Morm$\grave{e}$de and R. Geers. 2006, Validation of a behavioral observation tool to assess pig welfare, Physiol. Behav 89:438-447 https://doi.org/10.1016/j.physbeh.2006.07.002

Cited by

  1. Review: Minimally invasive sampling media and the measurement of corticosteroids as biomarkers of stress in animals vol.92, pp.3, 2012, https://doi.org/10.4141/cjas2012-045
  2. The Neuroendocrinology of Social Isolation vol.66, pp.1, 2015, https://doi.org/10.1146/annurev-psych-010814-015240