DOI QR코드

DOI QR Code

Every Single Cell Clones from Cancer Cell Lines Growing Tumors In Vivo May Not Invalidate the Cancer Stem Cell Concept

  • Li, Fengzhi (Departments of Pharmacology and Therapeutics, Roswell Park Cancer Institute)
  • Received : 2008.12.15
  • Accepted : 2009.02.16
  • Published : 2009.04.30

Abstract

We present the result of our research on the tumorigenic ability of single cell clones isolated from an aggressive murine breast cancer cell line in a matched allografting mouse model. Tumor formation is basically dependent on the cell numbers injected per location. We argue that in vivo tumor formation from single cell clones, isolated in vitro from cancer cell lines, may not provide conclusive evidence to disprove the cancer stem cell (CSC) theory without additional data.

Acknowledgement

Supported by : NIH, Charlotte Geyer Foundation

References

  1. Danovi, S.A. (2009). Cancer stem cells: common as muck. Nat. Rev. Cancer 9, 6-7 https://doi.org/10.1038/nrc2563
  2. Ewens, A., Luo, L., Berleth, E., Alderfer, J., Wollman, R., Hafeez, B.B., Kanter, P., Mihich, E., and Ehrke, M.J. (2006). Doxorubicin plus interleukin-2 chemoimmunotherapy against breast cancer in mice. Cancer Res. 66, 5419-5426 https://doi.org/10.1158/0008-5472.CAN-05-3963
  3. Quintana, E., Shackleton, M., Sabel, M.S., Fullen, D.R., Johnson, T.M., and Morrison, S.J. (2008). Efficient tumour formation by single human melanoma cells. Nature 456, 593-598 https://doi.org/10.1038/nature07567
  4. Visvader, J.E., and Lindeman, G.J. (2008). Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat. Rev. Cancer 8, 755-768 https://doi.org/10.1038/nrc2499
  5. Yoo, M.H., and Hatfield, D.L. (2008). The cancer stem cell theory: Is it correct? Mol. Cells 26, 514-516
  6. Zheng, X., Shen, G., Yang, X., and Liu, W. (2007). Most C6 cells are cancer stem cells: evidence from clonal and population analyses. Cancer Res. 67, 3691-3697 https://doi.org/10.1158/0008-5472.CAN-06-3912