Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Primary study of sterols composition of Rhodiola sachalinensis by using GC/MS

GC/MS를 이용한 고산 홍경천의 스테롤 구성에 대한 초기연구

  • Jin, Yinzhe (Department of Chemical Engineering, Inha University) ;
  • Li, Xifeng (Key Laboratory of Nature Resource and Functional Molecular of the Changbai Mountain, Ministry of Education) ;
  • Li, Donghao (Key Laboratory of Nature Resource and Functional Molecular of the Changbai Mountain, Ministry of Education) ;
  • Row, Kyung Ho (Department of Chemical Engineering, Inha University)
  • 김은철 (화학공학과, 인하대학교) ;
  • 이희봉 (장백산 천연자원 및 공능인자 교육부 중점 실험실 (연변대학교)) ;
  • 이동호 (장백산 천연자원 및 공능인자 교육부 중점 실험실 (연변대학교)) ;
  • 노경호 (화학공학과, 인하대학교)
  • Received : 2009.05.06
  • Accepted : 2009.05.27
  • Published : 2009.06.25
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Abstract

The steroid compounds in Rhodiola sachalinensis were determined with adsorption column chromatographic purification and GC/MS. Sterols were extracted by sonication and Soxhlet with ethanol and dichloromethane, respectively. The extract was partitioned with chloroform and water using liquid-liquid extraction, and purified with a silica column after the sterols had been converted to the corresponding silyl derivatives with BSTFA. Eighteen free sterols, including $\beta$-sitosterol, stigmasterol and cycloartenol, and nine sterol conjugates were found from Rhodiola sachalinensis by GC/MS. Among them, cholest-5-ene-3-ol, cholesterol, stigmasterol, $\beta$-sitosterol were confirmed and quantified with sterol standards. Most sterols were presented in the chloroform part, with $C_{29}$ being the most abundant group in this sterol group. $\beta$-sitosterol was the most abundant compound with a relative content of 45.94% followed by ergost-7-ene-3-ol (11.33%), 4,14-dimethyl-ergosta-8,24(28)-diene-3-ol (7.07%), stigmasterol (6.09%), cycloartenol (5.43%) and 4-methyl-cholest-5-ene-3-ol (5.39%).

고산 홍경천에 포함된 스테로이드 화합물의 구성을 흡착 컬럼 크로마토그래픽 정제와 GC/MS를 이용하여 측정하였다. 스테롤은 에탄올과 디클로로메탄을 각각 초음파와 Soxhlet의 용매로 사용하여 추출하였다. 추출물은 클로로포름과 물로 액-액 추출을 수행하여 분배하였으며, 실리카 컬럼으로 정제하였으며, BSTFA를 사용하여 silyl유도 반응을 수행하였다. GC/MS를 이용하여 고산 홍경천에서 $\beta$-sitosterol, stigmasterol과 cycloartenol을 포함한 18가지 자유 스테롤과 9가지 포합체 스테롤을 검출할 수 있었다. 그중에서 cholest-5-ene-3-ol, cholesterol, stigmasterol, $\beta$-sitosterol은 스테롤 표준품으로 확인하고 정량분석을 수행하였다. 대부분의 스테롤은 클로로포름 분액에서 검출되었고, $C_{29}$는 여러 그룹 중에서 가장 많은 그룹이었다. $\beta$-sitosterol은 가장 많이 함유된 성분이며 상대함량은 45.94%이고, 차례로 ergost-7-ene-3-ol (11.33%), 4,14-dimethyl-ergosta-8,24(28)-diene-3-ol (7.07%), stigmasterol (6.09%), cycloartenol (5.43%)과 4-methyl-cholest-5-ene-3-ol (5.39%)이었다.

Keywords

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