The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Neuroprotective Effect of Insamyangyung-tang

인삼양영탕(人蔘養營湯)의 산화적 stress에 대한 뇌세포 보호효과

  • Kim, Seung-Hyun (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Lee, Chang-Hoon (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Lee, Jin-Moo (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Cho, Jung-Hoon (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Jang, Jun-Bock (Dept. of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Lee, Kyung-Sub (Dept. of Oriental Medicine, Graduate School, Kyung Hee University)
  • 김승현 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 이창훈 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 이진무 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 조정훈 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 장준복 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 이경섭 (경희대학교 한의과대학 한방부인과학 교실)
  • Published : 2009.02.27
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Abstract

Purpose: Oxidative stress was thought to play a critical role in neurodegenerative disease. Many in vivo and in vitro reports explained the possible pathway of human aging. But in therapeutic aspects, there was no clear answers to prevent aging associated with neural diseases. In this study, we investigated the antioxidant and neuroprotective effects of the Insamyangyung-tang (IYT). Methods: To estimate the antioxidant effects, we carried out 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azinobis-(3- ethylbenzothiazoline-6- sulfonic acid (ABTS) radical cation decolorization assay, and measurement of total polyphenolic content. To evaluate neuroprotective effect of IYT in vitro. We performed thiazolyl blue tetrazolium bromide (MTT) assay, reactive oxygen species (ROS) creation in SH-SY5Y. Tyrosine hydroxylase (TH) immunocytochemistry, nitric oxide (NO) assay, and TNF-${\alpha}$ assay in primary rat mesencephalic dopaminergic neurons. Results: The $IC_{50}$ values were $571.6{\mu}g/m{\ell}$ and $202.3{\mu}g/m{\ell}$ in DPPH and ABTS assay respectively. Total polyphenolic content was 1.05%. In SH-SY5Y culture, IYT significantly increased the decreased cell viability by 6-OHDA at the concentrations of $10{\mu}g/m{\ell}$ in pre-treatment group, $10-100{\mu}g/m{\ell}$ in post-treatment group, and $100{\mu}g/m{\ell}$ in co-treatment group. The production of ROS induced by 6-OHDA was significantly inhibited in IYT treated group. In mesencephalic dopaminergic cell culture, the IYT group reduced the dopaminergic cell loss against 6-OHDA toxicity and the production of No and TNF-${\alpha}$ at the concentration of $0.2{\mu}g/m{\ell}$. Conclusion: These results showed that IYT has antioxidant and neuroprotectctive effects in the dopaminergic cells through decreasing the production of ROS, NO and TNF-${\alpha}$ which can cause many neurodegenerative changes in brain cell.

Keywords

References

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