The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Biological Control of Tea Anthracnose Using an Antagonistic Bacterium of Bacillus subtilis Isolated from Tea Leaves

  • Kim, Gyoung-Hee (Department of Plant Medicine, Sunchon National University) ;
  • Lim, Myoung-Taek (Department of Plant Medicine, Sunchon National University) ;
  • Hur, Jae-Seoun (Department of Environmental Education, Sunchon National University) ;
  • Yum, Kyu-Jin (Coenbio Institute of Research and Development) ;
  • Koh, Young-Jin (Department of Plant Medicine, Sunchon National University)
  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

An antagonistic bacterium of Bacillus subtilis BD0310 against Colletotrichum theae-sinensis was isolated from the phylloplane of tea trees at a tea plantation in Korea. SC (suspension concentrate)-type biofungicide was formulated with the antagonist. Cell viability and antifungal activity of B. subtilis were maintained in the formulation more than 12 months at room temperature. The antagonist was sensitive only to copper sulfate among the chemical pesticides currently registered for tea trees in Korea. Greenhouse application demonstrated that the biofungicide controlled more effectively the disease in a protective mode than in a curative mode. Field trial showed that alternate applications of the biofungicide and chemical fungicide were more effective in controlling tea anthracnose than single application of the biofungicide or chemical fungicide with less use of chemicals. This study suggests that the biofungicide of B. subtilis 8D0310 is an effective method for biological control of anthracnose in tea plantations.

Keywords

References

  1. Fravel, D. R. 2005. Commercialization and implementation of biocontrol. Annu. Rev. Phytopathol. 43:337-359 https://doi.org/10.1146/annurev.phyto.43.032904.092924
  2. Hynes, R. K. and Boyetchko, S. M. 2006. Research initiatives in the art and science of biopesticide formulations. Soil Bioi. & Biochem. 38:845-849 https://doi.org/10.1016/j.soilbio.2005.07.003
  3. Horikawa, T. 1986. Occurrence of resistance of tea gray blight pathogen, Pestalotiopsis logiseta Spegazzini to benzimidazole fungicides. Bull. Shizuoka Tea Ezpt. Stn. 12:9-14
  4. Horikawa, T. 1987. Effective fungicides and application time for control of tea gray blight caused by Pesta/otiopsis logiseta Speg. Tea Res. J 62:45-56
  5. lanisiewicz, W. and Korsten, L. 2002. Biological control of postharvest diseases of fruits. Annu. Rev. Phytopathol. 40:411-441 https://doi.org/10.1146/annurev.phyto.40.120401.130158
  6. Kim, G H., Oh. S.-O., Hur, J.-S., Yum, K. J. and Koh, Y. J. 2006. Optimum cultivation conditions for mass production of an antagonistic bacterium Bacillus subtilis BD031 0 for development of a microbial agent controlling gray blight of tea plants. Res. Plant Dis. 12:85-90 (in Korean) https://doi.org/10.5423/RPD.2006.12.2.085
  7. Kim, G H., Lim, M. T., Hur, l-S., Yum. K. 1. and Koh, Y. J. 2008. Control of gray blight of tea plants using a biofungicide. Res. Pant Dis. 14: 37-42. (in Korean) https://doi.org/10.5423/RPD.2008.14.1.037
  8. Koh, Y. J., Shin, G H. and Hur, J. S. 2001. Seasonal occurrence and development of gray blight of tea plants in Korea. Plant Pathol. J 17:40-44
  9. Korea Crop Protection Association. 2007. 2007 Manual of Pesticide. Korea Crop Protection Association. 1031 pp. (in Korean)
  10. Oh. S.-O., Kim, G H., Lim, K.-M., Hur, l-S. and Koh, Y. J. 2005. Disease progress of gray blight on tea plant and selection of a biocontrol agent from phylloplane of tea plant. Res. Plant Dis. 11: 162-166 (in Korean) https://doi.org/10.5423/RPD.2005.11.2.162
  11. Oniki, M., Hamaya, E. and Ando, Y. 1985. The actual rate of resistant strains of the tea anthracnose fungus Gloeosporium theae-sinensis against benzimidazole fungicides in Japan. Tea Res. Report 61 :7-11
  12. Oniki, M., N arisawa, N. and Ando, Y. 1986. Incidence of strains of tea gray blight fungi Pesta/otiopsis longiseta and Pesta/otiopsis theae resistant to benzimidazole fungicides in Japan. Tea Res. J 64:29-33
  13. Park, S. K., Park, K. B. and Cha, K. H. 1996. Gray blight of tea tree caused by Pestalotiopsis longiseta. Korean J Plant Pathol. 12:463-465
  14. Park, S. K. 1995. Anthracnose of tea tree caused by Colletotrichum theae-sinensis. Pl. Dis. Agric. 1:26-28. (in Korean)
  15. Sanjay, R., Ponmurugan, P. and Baby, U. J. 2008. Evaluation of fungicides and biocontrol agents against gray blight disease of tea in the field. Crop Protect. 27:689-694 https://doi.org/10.1016/j.cropro.2007.09.014
  16. Saravanakumar, D., Vijayakumar, C., Kumar, N. and Samiyappan, R. 2007. PGPR-induced defense responses in the tea plant against blister blight disease. Crop Protect. 26:556-565 https://doi.org/10.1016/j.cropro.2006.05.007
  17. Shin, G H., Choi, H. K., Hur, 1. S. and Koh, Y. J. 1999. First report on gray blight of tea plant caused by Pestalotiopsis theae in Korea. Plant Pathol. J. 15:308-310
  18. Shin, G H., Hur, J S. and Koh, Y. J. 2000. Chemical control of gray blight oftea in Korea. Plant Pathol. J. 16: 162-165

Cited by

  1. Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum vol.103, pp.1, 2013, https://doi.org/10.1007/s10482-012-9791-2
  2. Evaluation of various fungicides and microbial based biocontrol agents against bird’s eye spot disease of tea plants vol.32, 2012, https://doi.org/10.1016/j.cropro.2011.10.001
  3. Differential antagonistic responses of Bacillus pumilus MSUA3 against Rhizoctonia solani and Fusarium oxysporum causing fungal diseases in Fagopyrum esculentum Moench vol.205, 2017, https://doi.org/10.1016/j.micres.2017.08.012
  4. Isolation and identification of Bacillus subtilis strain YB-05 and its antifungal substances showing antagonism against Gaeumannomyces graminis var. tritici vol.85, 2015, https://doi.org/10.1016/j.biocontrol.2014.12.010
  5. vol.41, pp.1, 2019, https://doi.org/10.1080/07060661.2018.1564792