Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Extracts from Rhizopus oryzae KSD-815 of Korean Traditional Nuruk Confer the Potential to Inhibit Hypertension, Platelet Aggregation, and Cancer Metastasis in vitro

  • Lee, Sang-Jin (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Bae, Hyun-Jin (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Ryu, Ji-Yeon (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Lee, Dae-Young (Natural Products Chemistry Laboratory, Department of Biotechnology, Kyung Hee University) ;
  • Kim, Gye-Won (Research Laboratories, Kooksoondang Brewery Co., Ltd.) ;
  • Baek, Na-Min (Natural Products Chemistry Laboratory, Department of Biotechnology, Kyung Hee University) ;
  • Kwon, Moo-Sik (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Hong, Sung-Youl (Department of Genetic Engineering, Sungkyunkwan University)
  • Published : 2009.12.31
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Abstract

Rhizopus oryzae KSD-815 was isolated from nuruk that has been used to make Korean traditional wines. This study was performed to investigate the effect of cultures of R. oryzae KSD-815 on cardiovascular disorders and cancer metastasis. Firstly, these cultures were sequentially fractionationed with n-hexane (TAHe), ethylacetate (TAE), n-butanol (TAB), and $H_2O$ (TAW). The TAE inhibited the activity of angiotensin-converting enzyme (ACE) and TAB suppressed platelet aggregation in vitro. TAE and TAB inhibited cell motility of human breast cancer cells. Furthermore, TAW interrupted the formation of neovasculature and tube-like structure, and down-regulated the expression of angiogenic factors, basic fibroblast growth factor (bFGF), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), and hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) in breast cancer cells. These results indicated that cultures of R. oryzae KSD-815 display the inhibitory activities on hypertension, platelet aggregation, and metastasis, and suggest that these cultures might be further probed for the purposes as therapeutic agents or dietary supplements.

Keywords

References

  1. Agli MD, Busciala A, Bosisio E. Vascular effects of wine polyphenols. Cardiovasc. Res. 63: 593-602 (2004) https://doi.org/10.1016/j.cardiores.2004.03.019
  2. Renaud S, de Lorgeril M. Wine, alcohol, platelets, and the French paradox for coronary artery disease. Lancet 339: 1523-1526 (1992) https://doi.org/10.1016/0140-6736(92)91277-F
  3. Tunstall-Pedoe H, Kuulasmaa K, Mahonen M, Tolonen H, Ruokokoski E, Amouyel P. Contribution of trends in survival and coronary-event rates to changes in coronary heart disease mortality: 10-years results from 37 WHO MONICA project populations: Monitoring trends and determinants in cardiovascular disease. Lancet 353: 1547-1557 (1999) https://doi.org/10.1016/S0140-6736(99)04021-0
  4. Shon SK, Rho YH, Kim HJ, Bae SM. Takju brewing of uncooked rice starch using Rhizopus koji. Korean J. Appl. Microbiol. Biotechnol. 18: 506-510 (1990)
  5. Yu TS, Yeo SH, Kim HS. A new species of hypmycetes, Aspergillus coreanus sp. nov., isolated from traditional Korean nuruk. J. Microbiol. Biotechn. 14: 182-187 (2004)
  6. Jeon BY, Kim SJ, Kim DH, Na BK, Park DH, Tran HT, Zhang R, Ahn DH. Development of a serial bioreactor system for direct ethanol production from starch using Aspergillus niger and Saccharomyces cerevisiae. Biotechnol. Bioprocess Eng. 12: 566-573 (2007) https://doi.org/10.1007/BF02931356
  7. Yoon CG, Chae SN, Huh NE, Kim HS, Yu TS. Effects of nuruk or wheat bran supplemented diet on the serum levels of cholesterol and activities of hepatic oxygen free radical metabolizing enzymes rats. J. Korean Soc. Food Sci. Nutr. 28: 212-217 (1999)
  8. Jung H, Kang JC. Documental studies on anti-cancer therapy by using medi-alcohol. J. Korean Oncol. 5: 119-136 (1999)
  9. Kim HR, Ahn BH. Research trend of Korean traditional alcoholic beverage. Food Ind. Nutr. 6: 5-10 (2001)
  10. Lee SJ, Kwon YJ, Kim HR, Ahn BH. Chemical and sensory characterization of Korean commercial rice wines (yakju). Food Sci. Biotechnol. 16: 374-380 (2007)
  11. Kim JH, Lee DH, Choi SY, Lee JS. Characterization of physiological functionalities in Korean traditional liquors. Korean J. Food Sci. Technol. 34: 118-122 (2002)
  12. Kim JH, Lee JH, Kim HJ, Choi SY, Lee JS. Effects of barley koji and legumes on the quality and fibrinolytic activity of Korean traditional rice wine. J. Korean Soc. Food Sci. Nutr. 32: 1066-1070 (2003) https://doi.org/10.3746/jkfn.2003.32.7.1066
  13. Kim JH, Jeong SC, Kim NM, Lee JS. Effect of Indian millet koji and legumes on the quality and angiotensin I-converting enzyme inhibitor activity of Korean traditional rice wine. Korean J. Food Sci. Technol. 35: 733-737 (2003)
  14. Chung HS, Hong SH, Do KR, Rhee HK, Jung SK, Hwang WJ, Kim HM. MooPoong (Gye Young Jeong) increases HDLcholesterol but decreases LDL cholesterol and body weight. Immunopharm. Immunot. 26: 225-232 (2004) https://doi.org/10.1081/IPH-120037718
  15. Chung KS, Oh WT, Nam SM, Son BS, Park YS. Effect off Korean rice-wine (yakju) on in vitro and in vivo progression of B16BL6 mouse melanoma and HRT18 human colon adenocarcinoma cells. Korean J. Food Sci. Technol. 30: 1470-1475 (1998)
  16. Kim SJ, Ko SH, Lee WY, Kim GW. Cytotoxic effects of Korean rice-wine (yakju) on cancer cells. Korean J. Food Sci. Technol. 36: 812-817 (2004)
  17. Kim SJ, Baek JY, Park CK, Kim GW. Gastroprotective effect of Korean rice-wine (yakju). Korean J. Food Sci. Technol. 36: 818-822 (2004)
  18. Cushman DW, Cheung HS. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 (1971) https://doi.org/10.1016/0006-2952(71)90292-9
  19. Born GV, Cross MJ. The aggregation of blood platelets. J. Physiol. 168: 178-195 (1963)
  20. Oikawa T, Hirotani K, Nakamura O, Shudo K, Hiragun A, Iwaguchi T. A highly potent antiangiogenic activity of retinoids. Cancer Lett. 48: 157-162 (1998) https://doi.org/10.1016/0304-3835(89)90054-2
  21. Li GH, Liu H, Shi YH, Le GW. Direct spectrophotometric measurement of angiotensin I-converting enzyme inhibitory activity for screening bioactive peptides. J. Pharmaceut. Biomed. 37: 219-224 (2005) https://doi.org/10.1016/j.jpba.2004.11.004
  22. Das UN. Essential fatty acids and their metabolites could function as endogenous HMG-CoA reductase and ACE enzyme inhibitors, anti-arrhythmic, anti-hypertensive, anti-atherosclerotic, and antiinflammatory, cytoprotective, and cardioprotective molecules. Lipids Health Dis. 15: 7-37 (2008)
  23. Oqawa A, Suzuk Y, Aoyama T, Takeuchi H. Dietary alpha-linoleic acid inhibits angiotensin-converting enzyme activity and mRNA expression levels in the aorta of spontaneously hypertensive rats. J. Oleo Sci. 58: 355-360 (2009) https://doi.org/10.5650/jos.58.355
  24. Frarndale RW, Sixma JJ, Barnes MJ, De Groot PG. The role of collagen in thrombosis and hemostasis. J. Thromb. Haemost. 2: 561-573 (2004) https://doi.org/10.1111/j.1538-7836.2004.00665.x
  25. Sarratt KL, Chen H, Zutter MM, Santoro SA, Hammer DA, Kahn ML. GPVI and ${\alpha}_2{\beta}_1$ play independent critical roles during platelet adhesion and aggregate formation to collagen under flow. Blood 106: 1268-1277 (2005) https://doi.org/10.1182/blood-2004-11-4434
  26. Jackson SP, Nesbitt WS, Kulkarni S. Signaling events underlying thrombus formation. J. Thromb. Haemost. 1: 1602-1612 (2003) https://doi.org/10.1046/j.1538-7836.2003.00267.x
  27. Emms H, Lewis GP. The role of prostaglandin endoperoxides, thromboxane A2, and adenosine diphosphate in collagen-induced aggregation in man and the rat. Brit. J. Pharmacol. 87: 109-115 (1986) https://doi.org/10.1111/j.1476-5381.1986.tb10162.x
  28. Hu K, Liu Q, Wang S, Ding K. New oligosaccharides prepared by acid hydrolysis of the polysaccharides from Nerium indicum Mill and their anti-angiogenesis activities. Carbohyd. Res. 26: 198-203 (2009)
  29. Ma J, Xin X, Meng L, Tong L, Lin L, Geng M, Ding J. The marinederived oligosaccharide sulfate (MdOS), a novel multiple tyrosine kinase inhibitor, combats tumor angiogenesis both in vitro and in vivo. PloS One 3: e3774 (1-11) (2008)
  30. Cole CL, Jayson GC. Oligosaccharides as anti-angiogenic agents. Expert Opin. Biol. Th. 8: 351-362 (2008) https://doi.org/10.1517/14712598.8.3.351
  31. Jubb AM, Pham TQ, Handy AM, Frantz GD, Peale FV, Wu TD, Koeppen HW, Hillan KJ. Expression of vascular endothelial growth factor, hypoxia inducible factor 1$\alpha$, and carbonic anhydrase IX in human tumours. J. Clin. Pathol. 57: 504-512 (2004) https://doi.org/10.1136/jcp.2003.012963
  32. Shi YH, Bingle L, Gong LH, Wang YX, Corke KP, Fang WG. Basic FGF augments hypoxia induced HIF-1-$\alpha$ expression and VEGF release in T47D breast cancer cells. Pathology 39: 396-400 (2007) https://doi.org/10.1080/00313020701444549
  33. Hellwig-Burgel T, Stiehl DP, Wagner AE, Metzen E, Jelkmann W. Hypoxia-inducible factor-1 (HIF-1): A novel transcription factor in immune reactions. J. Interf. Cytok. Res. 25: 297-310 (2005) https://doi.org/10.1089/jir.2005.25.297