Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effect of Paprika and Zooplankton Supplementation in the Diets on Carotenoid Deposition and Skin Pigmentation of the Rose Bitterling Rhodeus ocellatus

사료내 Paprika와 동물플랑크톤 혼합첨가가 흰줄납줄개 Rhodeus ocellatus의 Carotenoid 축적 및 체색에 미치는 영향

  • Lee, Choong-Ryul (Faculty of Marine Bioscience & Technology, Kangnung National University) ;
  • Bae, Ki-Min (Faculty of Marine Bioscience & Technology, Kangnung National University) ;
  • Lee, Sang-Min (Faculty of Marine Bioscience & Technology, Kangnung National University)
  • 이충열 (강릉대학교 해양생명공학부) ;
  • 배기민 (강릉대학교 해양생명공학부) ;
  • 이상민 (강릉대학교 해양생명공학부)
  • Published : 2009.02.25
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Abstract

This study was conducted to evaluate the effect of dietary supplementation of paprika powder as carotenoid source with three different zooplankton on carotenoid deposition and skin pigmentation of rose bitterling Rhodeus ocellatus. Two replicate groups of fish (initial average weight 0.8 g) were fed the five experimental diets containing 5% paprika (P5) and mixture of 5% paprika with 3% three zooplankton of water flea Moina macrocopa (P5M3), Artemia nauplius Artemia sp. (P5A3) and rotifer Brachionus plicatilis (P5R3) and without supplementation of carotenoid source (Con) for 10 weeks. Survival of fish among the treatments was not affected by dietary supplementation. Total carotenoid contents of whole body in fish fed the diets containing 3% zooplankton was higher than that of fish fed other diets. Especially, the highest total carotenoid contents of whole body was observed in fish fed the P5A3 diet. Similarity, Lightness, $a^*$ and $b^*$ values of the fish fed the diets containing 3% zooplankton tended to be higher than that in fish fed control and P5 diets. The apparent color intensity of skin and fin in fish fed the diets containing paprika with Artemia nauplius and rotifer was better than those of fish fed other diets. The results of this study suggest that dietary supplementation of paprika with Artemia nauplius and rotifer can improve skin pigmentation of rose bitterling.

본 연구는 사료내 색소원으로 paprika와 동물플랑크톤 혼합첨가가 흰줄납줄개의 carotenoid 축적 및 색택에 미치는 영향을 조사하였다. 대조사료(Con)를 제외한 모든 실험사료에 paprika 분말 5%를 첨가하고, paprika 분말과 동물플랑크톤의 상호작용을 알아보기 위하여 paprika 분말만 첨가한 실험사료(P5), P5사료에 water flea Moina macrocopa 3% 첨가사료(P5M3), Artemia nauplius (Artemia sp.) 3% 첨가사료(P5A3), rotifer Brachionus Plicatilis 3% 첨가사료(P5R3)로 총 5개의 실험사료를 제조한 후, 2반복으로 10주간 사육실험 하였다. 실험종료 후, 모든 실험구간에서 생존율의 차이는 없었으며, 총 carotenoids 함량은 동물플랑크톤이 첨가된 실험 구에서 높은 다소 높은 값을 보였다. 특히, P5A3 실험구에 가장 높은 값을 나타냈다. 흰줄납줄개의 색택은 paprika와 동물플랑크톤 혼합 첨가한 실험구가 대조구 및 P5 실험구에 비해 붉은색이 더 선명하였다. 이상의 결과를 고려할 때, 흰줄납줄개의 배합사료내 paprika와 동물플랑크톤의 혼합첨가는 붉은 색 개전에 도움이 되었고, 특히 Artemia nauplius 또는 rotifer 첨가가 좋을 것으로 판단된다.

Keywords

References

  1. Ahmadi, M. R., S. H. Safi, B. Bjerkeng，A. Gerami and R. Khodarahmi, 2005. Caspian sea gammarus (Pontogammarus maeoticus) as a carotenoid source for muscle pigmentation on rainbow trout (Oncorhynchμs mykiss). Iranian J. Fish. Sci., 5, 1-12
  2. AOAC, 1990. Official methods of analysis. 15th edition. Association of official analytical chemists. arlington, Virginia. pp. 1298
  3. Atalah, E., C. M. Hemandez Cruz, M. S. Izquierdo, G. Rosenlund, M. J. Caballero, A. Valencia and L. Robaina, 2007. Two microalgae Cηpthecodinium cohnii and Phaeodactylum tricornutum as altemative source of essential fatty acids in starter feeds for seabream (Sparus aurata). Aquaculture, 270, 178-185 https://doi.org/10.1016/j.aquaculture.2007.04.009
  4. Becker, E. W. and L. V. Venkataraman，1984. Production and utilization of the blue green alga Spirulina in India. Biomass, 4, 105-125 https://doi.org/10.1016/0144-4565(84)90060-X
  5. Booth, M. A., R. J. Warner-Smith, G L. Allan and B. D. Glencross, 2004. Effects of dietary astaxanthin source and light manipulation of the skin colour of Australian snapper Pagrus auratus (Bloch and Schneider, 1801). Aquacult. Res., 35, 182- 190
  6. Choubert, G and T. Storebakken, 1989. Dose response to astaxanthin and canthaxanthin pigmentation of rainbow trout fed various dietary carotenoid concentrations. Aquaculture, 81, 69-77 https://doi.org/10.1016/0044-8486(89)90231-7
  7. Chun, C.-Z., H.-G Park, S.-B. Hur and Y.-T. Kim, 1996. Biochemical studies of an endoglucanase from marine rotifer, Brachionus plicatilis. J. Aquacult., 9, 453-459
  8. Ciferri, O., 1983. Spirulina, the edible microorganism. Microbiol Rev., 47, 551-578
  9. Gouveia, L., P. Rema, O. Pereira and J. Empis, 2003. Colouring omametal fish (Cyprinus carpio and Carassius auratus) with microalgal biomass. Aquacult. Nutr., 9, 123-129 https://doi.org/10.1046/j.1365-2095.2003.00233.x
  10. Guillaume, J., S. Kaushik, P. Bergot and R. Metailler, 2001. Nutrition and feeding of fish and crustacans. Springer Verlag, pp.183-196
  11. Hancz, C., I. Magyary, T. Molnar, S. Sato, P. Hom and N. Taniguchi, 2003. Evaluation of color intensity enhanced by paprika as feed additive in goldfish and koi cqrp using computer- assisted image analysis. Fish. Sci., 69, 1158-1161 https://doi.org/10.1111/j.0919-9268.2003.00740.x
  12. Khozin-Goldberg, I., Z. Cohen, M. Pimental-Leibowitz, J. Nechev and D. Zilberg, 2006. Feeding with arachidonic acid-rich triacylglycerols from the microalga Parietochloris incisa improved recovery of guppies from infection with Tetrahymena sp. Aquaculture, 255, 142-150 https://doi.org/10.1016/j.aquaculture.2005.12.017
  13. Kim, S.-R., C.-R. Leε and S.-M. Lee, 2006. Effect of dietary supplementation of paprika and Sirulina on pigmentation of swiri Coreoleuciscus splendidus. J. Aquacult., 19, 261-266
  14. Kim, Y.-O., J.-Y. Jo and S.-Y. Oh, 2008. Effects of dietary Spirulina, Chlorella, and astaxanthin on the body color of red- and white-colored Carp, Cyprinus carpio. J. Kor, Fish. Soc., 41, 193-200
  15. Lee, S.-M., D. J. Kim, K. D. Kim, J. K. Kim and J. H. Lee, 2000. Growth and body composition of larval ayu (Plecoglossus altivelis) fed the micro-diets containing Kluyveromyces fragilis and Candida utilis. J. Kor. Fish. Soc., 33, 20-24
  16. Lee, C.-R. and S.-M. Lee, 2008. Effect of dietary supplementation of pigment sources on pigmentation of round tailed paradise fish Macropodus chinensis and pale chub Zacco platyptus. J. Aquacult., 21, 213-217
  17. Moon, J.-H. and J.-K. Kim, 1998. The disruption ofyeast cell wall by chemical treatrnent. Kor. J. Life Sci., 8, 197-202
  18. Park, J.-H., C.-S. Kim and S.-K. Noh, 2005. The effect of fresh paprika and paprika powder dried by far-infrared ray on inhibition oflipid oxidation in Lard Model System. Kor. J. Food Cook. Sci., 21, 475-481
  19. Sommer, T., F. M. L. Da Souza and N. M. Morrissy, 1992. Pigmentation of adult rainbow trout, Oncorhynchus mykiss, using the green alga Haematococcus pluvialis. Aquaculture, 106, 63-74 https://doi.org/10.1016/0044-8486(92)90250-O
  20. Sommer, T., W. T. Potts and N. M. Morrissy, 1991. Utilization of microalgal astaxanthin by rainbow trout (Oncorhynchus mykiss). Aquaculture, 94, 79-88 https://doi.org/10.1016/0044-8486(91)90130-Y
  21. Tsushima, M., H. Nemoto and T. Matsuno, 1999. The accumulation of pigments from paprika and other sources in the integument of gold fish Carassius auratus. Fish. Sci., 64, 656-657