Separation and Identification of Antimicrobial Substances from Green Tea Extracts

녹차추출물로부터 항균물질의 분리 및 구조동정

  • Shin, Young-Hee (Department of Food Science and Technology.Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Seung-Cheol (Department of Food and Biotechnology, Kyungnam University) ;
  • Choi, Sung-Gil (Department of Food Science and Technology.Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho-Jin (Department of Food Science and Technology.Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Cho, Sung-Hwan (Department of Food Science and Technology.Institute of Agriculture and Life Science, Gyeongsang National University)
  • 신영희 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 이승철 (경남대학교 식품생명공학과) ;
  • 최성길 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 허호진 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 조성환 (경상대학교 식품공학과.농업생명과학연구원)
  • Published : 2009.12.30

Abstract

Green tea has been shown to have multifunctional health-promoting properties including cholesterol level control an antidiabetic effect and anticancer, antioxidant, and antimicrobial properties, inboth in vivo and in vitro experiments.We earlier reported antifungal and antibacterial effects of green tea extract by investigating cell membrane functions. The purpose of the present study was to purify and identify antimicrobial substances from green tea extract. Such materials were extracted from green tea (Camellia sinensis. var. sinensis) and purified by high-performance liquid chromatography. The antimicrobial substances in the extract were identified as epicatechin gallate and epigallocatechin gallate by nuclear magnetic resonance spectrophotometry.

녹차에 관한 최근까지의 연구결과에서, 녹차는 생리활성의 촉진작용뿐아니라 항암, 항산화, 고혈압 억제효과, 체중 조절 등 다방면의 약리효과를 보이는 것으로 보고되고 있다. 아울러, 녹차의 항균 및 항진균 작용에 관한 연구결과도 발표되고 있으며, 본 연구팀에 의해서도 녹차추출물의 항균작용을 확인한 바 있어, 본 연구에서는 탁월한 항균작용을 소유하고 있는 녹차분획물질을 분리하여 그 분자구조를 동정하는데 목적이 있다. 따라서, 녹차추출물에서 탁월한 항균력을 가지는 항균활성물질을 HPLC 분리방법 및 NMR 분석법으로 분리 동정한 결과, 각각 (-)-epicatechin gallate 및 (-)-epigallocatechin gallate로 확인되었다.

Keywords

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