Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Comparison of a PCR Kit and a Selective Medium to Detect Pathogenic Bacteria in Eggs

PCR Kit와 선택배지를 이용한 계란의 병원성세균 검출 비교 평가

  • Kim, Dong-Ho (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute) ;
  • Yun, Hye-Jeong (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Song, Hyun-Pa (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute) ;
  • Lim, Sang-Yong (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute) ;
  • Jo, Min-Ho (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute) ;
  • Jo, Cheo-Run (Department of Animal Science and Biotechnology, Chungnam National University)
  • 김동호 (한국원자력연구원 방사선과학연구소) ;
  • 윤혜정 (충남대학교 동물자원생명과학과) ;
  • 송현파 (한국원자력연구원 방사선과학연구소) ;
  • 임상용 (한국원자력연구원 방사선과학연구소) ;
  • 조민호 (한국원자력연구원 방사선과학연구소) ;
  • 조철훈 (충남대학교 동물자원생명과학과)
  • Published : 2009.12.30
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Abstract

PCR technology has been widely used to detect and quantify microbial pathogens in foodstuffs, because the technique is rapid, sensitive, and selective. In this study, detection of contaminating pathogenic bacteria on shells of chicken eggs was performed using both a commercial multiplex polymerase chain reaction (PCR) kit and a viable count method employing a selective medium. The PCR kit was capable of detecting Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Listeria monocytogenes, Yersinia enterocolitica, Salmonella species, and Shigella species. Using the PCR method, five bacterial species were detected from 30 samples (33.3%) of 90 batches of eggs commercially available in a market. PCR products from B. cereus, S. aureus, L. monocytogenes, Y. enterocolitica, and E. coli O157:H7 were detected, and the numbers and frequencies of positive samples were 17 (18.8%), 12 (13.3%), 15 (16.6%), 16 (17.7%),and 4 (4.4%), respectively. None of any Salmonella species, C. jejuni, V. parahaemolyticus, or Shigella species was detected in this study. The results of PCR testing were confirmed using a typical viable count method employing a selective medium. We suggest that the multiplex polymerase chain reaction (mPCR) assay is a rapid and reliable method for detection of pathogenic bacteria contaminating eggs.

유통중인 계란의병원성균 오염상태를 시판되는 multiplex polymerase chain reaction (mPCR) kit로 검사한 결과, 총 90개의 검사시료 가운데 한 가지 이상의 미생물이 검출된 계란시료는 30개로, 검사시료의 미생물 오염도는 약 33.3% 수준이었다. 미생물별 검출 빈도는 B. cereus가 17개 시료(18.9%)에서 검출되어 가장 높은 빈도를 보였으며 Y. enterocolitica는 16개(17.8%), L. monocytogenes 15개(16.7%), St. aureus 12개(13.3%), E. coli O157:H7 4개(4.4%)의 검출빈도를 보였다. 한편, 선택배지를 이용한 viable cell count 방법에서는 27개의 시료에서 미생물이 검출되어 검사시료의 미생물 오염도는 약 30.0% 수준이였으며, 미생물별 검출 빈도는 B. cereus가 17개 시료(18.9%)에서 14개(15.6%) 시료로, Y. enterocolitica는 16개(17.8%)에서 12개(13.3%)로, L. monocytogenes는 15개(16.7%)에서 13개(14.4%)로, St. aureus는 12개(13.3%)에서 10개(11.1%)로 감소하였으며, E. coli O157:H7은 두 가지 검출방법간의 차이가 나타나지 않았다. 검출 대상 미생물 9종 가운데 Campylobacter jejuni, Vibrio parahaemolyticus, Salmonella spp., 및 Shigella spp.는 두 방법 모두에서 검출되지 않았다. 이상의 결과에서 살펴본 바와 같이 PCR을 이용한 병원성 미생물의 검출 방법은 viable cell count 방법보다 감도가 높음을 알 수 있었다.

Keywords

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