Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Screening of Biological Activities of Ethanol Extracts from Several Varieties of Endives

품종별 치콘 추출물의 생리활성 탐색

  • 이광재 (강원도농업기술원 농산물이용시험장) ;
  • 박민희 (강원도농업기술원 농산물이용시험장) ;
  • 서현택 (강원도농업기술원 원예연구과) ;
  • 박유화 (강원도농업기술원 농산물이용시험장) ;
  • 권창주 (강원도농업기술원 농산물이용시험장) ;
  • 임상현 (강원도농업기술원 농산물이용시험장) ;
  • 김경희 (강원도농업기술원 농산물이용시험장) ;
  • 전신재 (강원도농업기술원 원예연구과) ;
  • 원재희 (강원도농업기술원 원예연구과)
  • Published : 2009.12.30
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Abstract

We investigated the biological activities of ethanol extracts obtained from five endive cultivars. Free radical-scavenging activities of extracts from Redoria, Halifax, Vintor, Kibora, and Metafora cultivars were 94.2%, 60.8%, 66.6%, 70.0% and 67.4%, respectively. The extract from Redoria showed the highest activity among all the tested samples including BHT (Butylated hydroxyanisole) and vitamine C, known as antioxidant. Endive extracts slightly (17.5 - 38.6%) inhibited $\alpha$-glucosidase activity. However, there is no inhibitory activity against $\alpha$-amylase. In terms of proteolytic activity, all endive extracts showed strong activity than pancreatin (used as a positive control), and the extract of Redoria also exhibited stronger activity than other extracts as well as free radical scavenging activity. These results indicate that endive, especially the Redoria cultivar, can be used as a natural resource for development of antioxidants or materials aiding digestion.

치커리의 종근으로 치콘을 재배하여 엽색이 노란색과 붉은색인 다섯 가지 종류의 치콘 추출물에 대한 항산화효과 및 혈당강하효과와 단백분해능을 측정하였다. DPPH free radical 소거능으로 항산화활성을 측정한 결과, 실험에 사용한 모든 시료가 우수한 항산화 활성을 가지고 있었으며 특히, 엽색이 노란색인 네 종류 (Kibora, Metafora, Vintor, Halifax)의 치콘보다 엽색이 붉은색인 Redoria종이 1 mg/mL의 농도에서 94.2%의 높은 항산화 활성을 나타냈다. 치콘 추출물의 혈당 조절능을 평가하기 위해, Porcine pancreas, Human saliva 및 Bacillus licheniformis 기원의 $\alpha$-amylase와 효모기원의 $\alpha$-glucosidase에 대한 추출물의 저해 활성을 조사한 결과, $\alpha$-amylase에서는 저해활성을 보이지 않은 반면에 $\alpha$-glucosidase에는 17.5~38.6%의 저해활성을 보였다. 치콘의 단백분해능을 측정한 결과, 실험에 사용한 다섯 종류의 치콘추출물 모두 대조품으로 사용한 Pancreatin보다 우수한 활성을 나타냈으며 항산화 활성과 마찬가지로 Redoria 추출물의 활성이 가장 우수하였다. 이러한 결과로 보아 치콘을 항산화제나 소화제 등의 천연 소재로 이용할 수 있을 것으로 사료된다.

Keywords

References

  1. Wiseman, H. (1996) Dietary influences on membrane function: Important in protection against oxidative damage and disease. Nutr. Biochem., 7, 2-6 https://doi.org/10.1016/0955-2863(95)00152-2
  2. Choi C.S., Song E.S., Kim J.S. and Kang M.H. (2003)Antioxidative activities of Castanea Ctenata Flos. Methanol extracts. Korean J. Food Sci. Technol., 35, 1216-1220
  3. Park, K.W. (1986) Western vegetable. Korea Univ. Publishing, 271-281
  4. Kim Y.B. (2006) Study on blanching culture of Chicory (Cichorium intybus L.) rootstock and storage methods of Chicon. Wonkwang Univ., a doctoral dissertation
  5. Franck A., Leenheer L. (2002) Polysaccharides II 'Inulin'. Biopolymers, 6, 439-480
  6. Ohta A., Ohtsuki M., Baba S., Hirayama M. and Adachi T. (1998) Comparison of the nutritional effects of fructooligosaccharides of different sugar chain length in rats. Nutr. Res., 18, 109-120 https://doi.org/10.1016/S0271-5317(97)00205-4
  7. Rhee S.K., Song K.B., Kim C.H., Park B.S., Jang E.K. and Jang K.H. (2002) Polysaccharides I. "Levan". Biopolymers, 5, 351-377 https://doi.org/10.1002/bip.1967.360050404
  8. Roberfroid M.B. and Delzenne N.M. (1998) Dietary fructans. Ann. Rev. Nutr., 18, 117-143 https://doi.org/10.1146/annurev.nutr.18.1.117
  9. Kim T.Y., Yoon Y.J. and Lee K.W. (1978) Studies on the constituents of the Chicory Root. Korean J. Food Sci. Technol., 10, 258-262
  10. Stevens, M.A. (1974) Varietal influence on nitritonal value. In:White, P.L. and Selvery, N.(eds) Nutritional qulities of fresh fruits and vegetable. Futura Publications, 87-109
  11. Kwon M.C., Han J.G., Qadir S.A., An J.H., Lee D.H. and Lee H.Y. (2008) Enhancement of immuno-potentiation of Cichorium endivia L. by ultrasonification extraction process. Korean J. Med. Crop Sci., 16, 1-7
  12. Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature, 26, 1198-1202
  13. Kwon S.K., Park S.W. and Choi W.Y. (1998) Properties of the proteolytic enzymes from Mulberry tree bark (Morus alba Linne). Korean J. Food Nutr., 11, 576-579
  14. Lim C.S., Li C.Y., Kim Y.M., Lee W.Y. and Rhee H.I. (2005) The inhibitory effect of Cornus walteri extract against α-amylase. J. Korean Soc. Appl. Biol. Chem., 48, 103-108
  15. Saija A. (1994) Pharmacological effects of anthocyanins from blood orange juice. Essenze-Deriv. Agrum., 64, 229-233
  16. Cho Y.J., Ju I.S., Kin B., Lee W.S., Kim M.J., Lee B.G.,An B.J., Kim J.H. and Kwon O.J. (2007) Biological activity of Omija (Schizandra chinensis Baillon) extracts. J. Korean Soc. Appl. Biol. Chem., 50, 198-203
  17. Woo N., Song E.S., Kim H.J., Seo M.S. and Kim A.J. (2007) The comparison of antioxidative activities of sprouts extract. Korean J. Food Nutr., 20, 356-362
  18. Choi N.Y. and Shin H.S. (2007) Physicochemical properties and physiological activities of Olive leaf (Olea europaea L. var. Kalamata) fractions. Food Eng. Prog., 11, 153-160
  19. Kim S.H., Choi H.S., Lee M.S. and Chung M.S. (2007)Volatile compounds and antioxidant activities of Adenophora remotiflora. Korean J. Food Sci. Technol., 39, 109-113
  20. Sessa R.A., Bennett M.H., Lewis M.J., Mansfield J.W.,Beale M.H. (2000) Metabolite profiling of sesquiterpene lactones from Lactuca species. Major latex components are novel oxalate and sulfate conjugates of lactucin and its derivatives. J. Biol. Chem., 275, 26877-26884
  21. Bischoff T.A., Kelley C.J., Karchesy Y., Laurantos M., Nguyen-Dinh P., Arefi A.G. (2004) Antimalarial activity of lactucin and lactucopicrin: sesquiterpene lactones isolated from Cichorium intybus L. J. Ethnopharmacol., 95, 455-457 https://doi.org/10.1016/j.jep.2004.06.035
  22. Wesołowska A., Nikiforuk A., Michalska K., Kisiel W.,Chojnacka-Wójcik E. (2006) Analgesic and sedative activities of lactucin and some lactucin-like guaianolides in mice. J. Ethnopharmacol., 107, 254-258 https://doi.org/10.1016/j.jep.2006.03.003
  23. Rollinger J.M., Mocka P., Zidorn C., Ellmerer E.P.,Langer T., Stuppner H. (2005) Application of the in combo screening approach for the discovery of non-alkaloid acetylcholinesterase inhibitors from Cichorium intybus. Curr. Drug Discov. Technol., 2, 185-193 https://doi.org/10.2174/1570163054866855
  24. Gua J., Jin Y.S., Han W., Shim T.H., Sa J.H. and Wang M.H. (2006) Studies for component analysis, Antioxidative and α-glucosidase inhibitory activity from Equisetum arvense. J. Korean Soc. Appl. Biol. Chem., 49, 77-81