Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Biological and Antifungal Activity of Herbal Plant Extracts against Candida Species

수종의 한약재 추출물의 항산화 활성 및 항진균 활성

  • Kim, Jae-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Yi, Yong-Sub (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Lim, Yoong-Ho (BMIC, Department of Bioscience and Biotechnology, Konkuk University)
  • 김재영 (호서대학교 한방화장품과학과) ;
  • 이용섭 (호서대학교 한방화장품과학과) ;
  • 임융호 (건국대학교 생명/분자정보학센터 생명공학과)
  • Published : 2009.03.28
Download PDF
⟨ Previous Next ⟩

Abstract

Anticandidial activity of seven herbal extracts, Taraxacum Platycarpum, Houttuyniae Herba, Lonicerae Flos, Anemarrhena Rhizome, Forsythia Fruit, Paeoniae Ratix, and Coptidis Rhizoma, were determined against five different Candida sp. by agar diffusion assay. The concentration of total phenolic compounds of seven herbal extracts ranged from 0.6 to $2.5{\mu}g/mg$. The total antioxidant activities showed that Taraxacum Platycarpum and Houttuyniae Herba were 60% in 80% ethanol extract and Lonicera Flower and Paeoniae Ratix were 70, 75%, respectively, in 100% ethanol extract. Coptidis Rhizoma extract showed antifungal activity against non-Candida albicans, C. tropicalis and C. glabrata. The MIC values of a compound separated in TLC from Coptidis Rhizoma extract were 24, and $48{\mu}g/mL$ against C. tropicalis and C. glabrata. The above compound showed the same retention time with berberin in HPLC analysis.

본 연구에서는 여성의 질염을 일으키는 칸디다증 병원균에 대한 항진균 성분을 한약재에서 조사하였다. 한약재에 ethanol을 이용하여 phenol 성분을 추출하고, 추출물의 phenol 함량을 출정하였다. Ethanol농도에 따른 phenol성 물질의 용출량은 시료마다 다른 양상을 보이고 있는데, 40% ethanol 농도에서 가장 높은 용출량은 어성초, 지모, 금은화 등이며, 60% ethanol에서 가장 높은 용출량은 포공영, 작약 등이다. 황련은 80% ethanol 농도에서는 낮은 용출량을 보이나 다른 농도에서는 비슷한 용출량을 보이고 있고, 연교는 80% ethanol에서 높은 용출량을 보였다. 전자공여능은 ethanol 농도별 추출물에 따라 다르게 나타나고 있는데, 포공영은 80% ethanol 추출물 이상에서 전자공여 능을 보이며, 황련, 지모, 금은화는 100%의 ethanol추출물에서 전자공여능을 보였다. 한약재 추출물의 항균활성은 C. albicans는 황련 추출물 $200{\mu}g/mL$농도로 주입하였을 때 3mm의 저해환을 나타내었으며, C. tropicalis는 포공영 추출물을 $200{\mu}g/mL$ 농도로 주입시 2mm, 어성초 추출물을 $200{\mu}g/mL$ 농도로 주입시 2mm, 작약 추출물을 $200{\mu}g/mL$ 주입시 3 mm 저해환을 나타내었다. 황련 추출물은 50, 100, 150, $200{\mu}g/mL$ 농도로 주입시 2, 3, 6, 9mm의 저해환을 나타내었다. 추출물을 이용한 C. glabrata, C. parapsilesis, C. utils 3개 종에 대한 항균활성은 나타나지 않았다. 황련 추출물에서 항균성분은 TLC와 HPLC 분석을 통하여 berberin인 것으로 나타났다.

Keywords

References

  1. An, B. J., J. T. Kee, C. E. Lee, J. H. Kim,J. B. Son, J. H. Kwak, J. Y. Lee, T. S. Park, H. J. Bae, M. J. Jang, and C. H. Jo. 2005. A study on physiological activities of Coptidis Rhizoma and application for cosmetics ingredients. Kor. J. Herbology 20: 83-92
  2. Bae,J. H., M. S. Kim, and E. H. Kang. 2005, Antimicrobiill effect of Lonicerae Flos extracts on food-bome pathogens. Korean J. Food Sci. Technol. 37: 642-647
  3. Blois, M. S. 1958. Antioxidant determination by the use of a stable free redical. Nature 26: 1199-1200
  4. Brand-Williams, W., M. E. Cuvelier, and C, Berset. 1995. Use of a free radical method to evaluate antioxidant activity. Food Science and Technology 28: 25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  5. Bravo, L. 1998. Polyphenols: Chemistty, dietary sources, metabolism, and nutritional significance. Nutrition Reviews 56: 317 https://doi.org/10.1111/j.1753-4887.1998.tb01670.x
  6. Dural, B. and K. Shetty.2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transforrned Anise root extract. J. food Biochem. 25: 361-377 https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  7. Hakkinen, S. H. and A. R. Torronen. 2000. Content of flavonols and selected phenolic acids in strawberries and Vaccinium species: Influence of cultivar, cultivation site and technique. Food Research International 33: 517-924 https://doi.org/10.1016/S0963-9969(00)00086-7
  8. Hattori, T, K. Furuta. T. Nagao, T. Nagarnatsu, T. Ito, and Y. Suzuki. 1992. Studies on the antinephritic effect of plant cornponents (4): Reduction of protein excretion by berberine and Coptisine in rats with origina1-type anti-GMB nephritis. Jpn. J. Pharmacol. 59: 156-169
  9. Hollman, P. C. H., M. G. L. Hertog, and K. B. Katan. 1996. Analysis and health effects of flavonoids. Food Chemistry, 57: 43-96 https://doi.org/10.1016/0308-8146(96)00065-9
  10. Hong, S. H. and S. S. Roh. 2005. Effects of herba1 extracts on the inflanunatory reactions which use the rnakeup preparations. Korean J. Oriental Physiology and Pathology. 19: 1419-1426
  11. Kirn, K. Y., D. O. Chung, and H. J. Chung. 1997. Chemical cornposition and antimicrobial activities of Houttuynia cordata THUNB. Korean J. Food Sci. Technol. 29: 400-406
  12. Kitagawa, I, O. M. Sankawa, M. Tomoda, and I. Nishioka. 1982. Pharrnacology. Tokyo, Japan, 2nd Ed., pp. 234-236
  13. Kong, J. M., L. S. Chia, N. K. Goh, T. F. Chia, and R. Brouillard. 2003. Ana1ysis and biologica1 activities of anthocyanins. Phytochemistry, 64: 923-933 https://doi.org/10.1016/S0031-9422(03)00438-2
  14. Kuo, C. L., C. W. Chi, and T. Y. Liu. 2004. The anti-inflarnrnatory potentia1 of berberine in vitro and in vivo. Cancer Lett. 203: 127-137 https://doi.org/10.1016/j.canlet.2003.09.002
  15. Larsen, B. and G R. Motif. 2001. Understanding the bacteria1 flora of the ferna1e genita1 tract. Clin. lnfect Dis. 32: 69-77 https://doi.org/10.1086/318710
  16. Lee, J. H., S. I. Jeong, I. S. You, and S. K. Kim. 2001. The inhibitory effects of the methanol extracts of Houttuynia cordata THUNB against cadmium induced cytotoxicity (V). Kor. J. Pharmacogn 32: 61-67
  17. Lee, J. S., B. S. Min, and K. W. Bae. 1996. Effects of Forsythiae fructus on L1210 and HL-60 cells. Yakhak Hoeji 40: 462-467
  18. Liu, B., W. Li, Y. Chang, Y. Dong, and L. Ni. 2006. Extraction of berberine from rhizome of Coptis cmnensis Franch using supercritical fluid extraction. J Pharm Biomed Anal. 41: 1056-1060 https://doi.org/10.1016/j.jpba.2006.01.034
  19. Monagas, M., B. Bartolome, and C. Gomez-Cordoves. 2005. Updated knowledge about the presence of phenolic compounds in wine. Critical Revìews in Food Science and Nutrition 45: 85-118 https://doi.org/10.1080/10408690490911710
  20. Reichart, P. A., L. P. Samaranayake. Y. H. Sarnaranayake, M. Grote, E. Pow, and B. Cheung. 2002. High ora1 prevalence of Candida krusei in leprosy patients in northem Thailand. J. Clin. Microbiol. 40: 4479-4485 https://doi.org/10.1128/JCM.40.12.4479-4485.2002
  21. Robbins, R. J. 2003. Phenolic acids in foods: An overview of anaIyticaI methodology. J. Agric. Food Chem. 51: 2866-887 https://doi.org/10.1021/jf026182t
  22. Ruan, W., M.Lai, and J. Zhou. 2006. Anticancer effects of Chinese herbaI medicine, science or myth? J. Zhejiang Univ Sci B. 7: 1006-1014 https://doi.org/10.1631/jzus.2006.B1006
  23. Seneviratne, C. J., R. W. K. Wong, and L. P. Sarnaranayake. 2007. Potent anti-microbiaI activity of traditionaI chinese medicine herbs against Candida species. Mycoses 51: 30-34 https://doi.org/10.1111/j.1439-0507.2008.01584.x
  24. Song, J. H., M. J. Kirn, H. D. Kwon, and I. H. Park. 2003. AntirnicrobiaI activity of fractionaI extracts frorn Houttuynia cordata root. J. Korean Sco. Food Sci. Nutri. 32: 1053-1058 https://doi.org/10.3746/jkfn.2003.32.7.1053
  25. World health organization. 1997. Lead environrnental heaIth criteria 3. Geneva, WHO. p. 160
  26. Zhanel, G G , J. A. Karlowsky, S. A. Zelenitsky, M. A. Turik, and D. J. Hoban. 1998. Susceptibilities of Candida species isolated from the lower gastrointestinaI tracts of high-risk patients to the new sernisynthetic echinocandin LY303366 and other antifungaI agents. Antimicrob. Agents Chemother. 42: 2446-2448