Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Oral-Administered 17α-Methyltestosterone and Estradiol-17β for Sex Reversal of Hybrid Sturgeon, Bester Juvenile

베스테르 철갑상어 치어 성전환을 위한 17α-methyltestosterone과 estradiol-17β 경구투여 효과

  • Kwon, O-Nam (Marine Biology Center for Research & Education, Gangneung-Wonju National University) ;
  • Adachi, Shinji (Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University)
  • 권오남 (강릉원주대학교 해양생물연구교육센터) ;
  • 아다찌신지 (북해도대학 대학원수산과학연구원)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to examine the effects of oral-administered sex hormone for hybrid sturgeon, bester juvenile. The bester juveniles (2 months after hatching) were received a diet containing various doses of $17\alpha$-methyltestosterone (MT) or estradiol-$17\beta$ ($E_2$) for 6 months. Somatic growth of bester sturgeon juvenile did not show significant differences between experimental and control groups (27.9-30.5 cm; 125.1-161.7 g), although survival percentages showed a decreasing tendency in MT-treated animals. By histological examination, germ cells were recorded as smooth type in MT-treated fish and uneven type of germinal epithelium in $E_2$-treated animals. Their sex ratios were 5:4:1 (male: female: undifferentiation) in control and low dose of MT-treated fish (1 mg/kg), and 9:1:0 in fish treated with high dose of MT (10 mg/kg), whereas the ratios were reversed by both low and high doses of $E_2$ treatment, recorded as 2:8:0. Gonadal areas were not significantly differed in all trials (424,600.4 - 1,039,656.3 ${\mu}m^2$). Total number of germ cells, number of germ cells per gonadal areas and number of germ cells per area were significantly higher to 144.7-148.7 cells/section, 374.0-408.5 $cells/mm^2$ and 1,599.5-1,670.9 $cells/mm^2$ in $E_2$ treatment than those of others (30.4-63.9 cells/section, 148.4-226.9 $cells/mm^2$ and 850.0-1,050.6 $cells/mm^2$), respectively. And somatic growth according to their gender was not significantly differed between male and female.

Keywords

References

  1. Bang IC, Park SY, Lee Y-A, Lee C-H, Kim S-Y and im K-K. 2000. Early gonadogenesis and sex differentiation in sweet fish, Plecoglossus altivelis. J Aquaculture 13, 215-222
  2. Duncan DB. 1955. Multiple-range and multiple F tests. Biometrics 11, 1-42 https://doi.org/10.2307/3001478
  3. Eenennaam ALV, Eenennaam JPV, Medrano JF and oroshov SI. 1996. Rapid verification of meiotic ynogenesis and polyploidy in white sturgeon Acipenser transmontanus Richardson). Aquaculture 47, 177-189
  4. Francis RC. 1992. Sexual ability in teleosts: developmental factors. Suart. Rev Biol 67, 1-17 https://doi.org/10.1086/417445
  5. Goetz FW, Donaldson EM, Hunter GA and Dye HM. 1979. Effets of estradiol-17$\beta$ and 17$\alpha$-methyltestosterone on gonadal differentiation in the coho salmon, Oncorhynchus kistch. Aquaculture 17, 267-278 https://doi.org/10.1016/0044-8486(79)90083-8
  6. Ijiri S, Kaneko H, Kobayashi T, Wang D-S, Sakai F, Paul-Prasanth B, Nakamura M and Nagahama Y. 2008. Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis nioticus. Biol Reproduct 78, 333-341 https://doi.org/10.1095/biolreprod.107.064246
  7. Komatsu T, Nakamura S and Nakamura M. 2006. Masculinization of female golden rabbitfish Siganus guttatus using an aromatase inhibitor treatment during sex differentiation. Comp Biochem Physiol 143, 402-409
  8. Kowtal GV. 1987. Preliminary experiments in induction of polyploidy gynogenesis, and androgenesis in the white sturgeon, Acipenser transmontanus Richardson. Tiews K. Proceedings of the World Symposium of Selection Hybridization and Genetic Engineering in Aquaculture 27-30 May 1986, Borodeaux, vol. 2. Heenemann Verlagsgesellschaft, Berlin, pp. 317-324
  9. Lee Y-D, Rho S, Chang YJ, Baek HJ and An C-M. 1996. Sex differentiation of the rockfish, Sebastes schegeli. J Korean Fish 29, 44-50
  10. Nakamura M, Kobayashi T, Chang X-T and Nagahama Y. 1998. Gonadal sex differentiation in teleost fish. J Exp Zool 281, 362-372 https://doi.org/10.1002/(SICI)1097-010X(19980801)281:5<362::AID-JEZ3>3.0.CO;2-M
  11. Omoto N, Maebayashi M, Mitsuhashi E, Yoshitomi K, Adachi S, and Yamauchi K. 2001. Histological observations f gonadal sex differentiation in the $F_2$ hybrid sturgeon, the bester. Fish Sci 67, 1104-1110 https://doi.org/10.1046/j.1444-2906.2001.00367.x
  12. Omoto N, Maebayashi M, Mituhashi E, Yoshitomi K, Adachi S and Yamauchi K. 2002a. Effects of estradiol-17$\beta$ and 17$\alpha$-methyltestosterone on gonadal sex differentiation in the $F_2$ hybrid sturgeon, the bester. Fish Sci 68, 1047-1054 https://doi.org/10.1046/j.1444-2906.2002.00531.x
  13. Omoto M, Maebayashi M, Adachi S, Arai K and Yamauchi K. 2002b. Histological obervations of gonadal development in gynogenetic diploids and triploids of the hybrid gturgeon, the bester. Fish Sci 68, 1271-1272 https://doi.org/10.2331/fishsci.68.sup2_1271
  14. Omoto M, Maebayashi M, Adachi S, Arai K and Yamauchi K. 2005. Sex ratios of triploids and gynogenetic diploids induced in the hybrid sturgeon, the bester (Huso huso female×Acipenser ruthenus male). Aquaculture 245, 39-47 https://doi.org/10.1016/j.aquaculture.2004.12.004
  15. Romachov DD, Nikolyukin NI, Belyaeva VN, Timofeeva NA. 1963. Possibilities of producting diploid radiation-induced gynogenesis in sturgeons. Radiobiologiya 3, 104-109
  16. Tanaka H. 1988. Effects of estradiol-17$\beta$ on gonadal sex differentiation in flounder, Paralichthys olivaceus. Bull Natl Res Inst Aquaculture 13, 17-23
  17. Uchida D, Yamashita M, Kitano T and Iguchi T. 2004. An aromatase inhibitor or high water temperature induce oocyte apoptosis and depletion of P450 aromatase activity in the gonads of genetic female zebrafish during sex-reversal. Comp Biochem Physiol 137, 11-20
  18. Van Enennaam AL, Van Enennaam JP, Medrano JF, Dprpshov SI. 1996. Rapid verfication of meiotic gynogenesis and polyploidy in white sturgeon (Acipenser transmontanus Richardson). Aquaculture 147, 177-189 https://doi.org/10.1016/S0044-8486(96)01369-5
  19. Yamamoto T 1959. The effects of estrone dosage level upon the percentage of sex-reversals in genetic male (XY) of the medaka (Oryzias latipes). J Exp Zool 41, 133-154