Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Post-Thaw Cryosurvival of Bovine Embryos Produced In Vitro and In Vivo after Controlled Freezing

  • Cho, Sang-Rae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choi, Sun-Ho (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Lee, Poong-Yeon (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, Jun-Kyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Jae-Bum (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Sung-Jae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyun-Jong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Shin, Seung-Oh (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, Dong-Soo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • Published : 2009.12.31
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Abstract

To enhance the embryo preservation technology and better application of embryo transfer technique to the field (dairy science or animal reproduction. etc.), we examined the viabilities of bovine embryos produced in vitro and in vivo after cryopreservation according to their developmental stage and thawing temperature. Bovine embryos from in vivo/vitro fertilization (Hanwoo) were examined at day 7, 8, and 9. Survival rates and total cell numbers of in vivo fertilized embryos were as follows: morulae 68.8% and $67\;{\pm}\;6.0$; blastocysts 80.5% and $120\;{\pm}\;10$; expanded blastocysts 77.4% and $138\;{\pm}\;9.7$, respectively. Rates of embryo development for blastocysts and expanded blastocysts after thawing were significantly higher than that of morula stage embryos (p<0.05). While survival rates of in vitro fertilized embryos according to developmental stage showed no significant difference among groups (morula 67.9%; blastocyst 74.3%; and expanded blastocyst 79.4%), total cell numbers were significantly lower than those of other groups (morula $64\;{\pm}\;5.9$; blastocyst $116\;{\pm}\;8.7$; and expanded blastocyst $135\;{\pm}\;9.1$) For the viability according to thawing temperature, survival rate was higher in $37^{\circ}C$.

Keywords

References

  1. Campos-Chillon LF, Walker DJ, Torre-Sanchez JF de la and Seidel Jr GE. 2006. In vitro assessment of direct transfer vitrification procedure for bovine embryos. Theriogenology 65:1200-1214 https://doi.org/10.1016/j.theriogenology.2005.07.015
  2. Dobrinsky JR, Johnson LA and Rath D. 1996. Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development. Biol. Reprod. 55:1069-1074 https://doi.org/10.1095/biolreprod55.5.1069
  3. Fashing ML and Garcia MA. 1992. Status of cryopreservation of bovine embryos from domestic animals. Cryobiology 29:1-18 https://doi.org/10.1016/0011-2240(92)90002-J
  4. Hasler JF. 2003. The current status and future of commercial embryo transfer in cattle. Anim. Reprod. Sci. 15:245-264 https://doi.org/10.1016/S0378-4320(03)00167-2
  5. Holm P and Callesen H. 1998. In vivo versus in vitro produced bovine ova: similarities and differences relevant for practical application. Reprod. Nutr. Dev. 38:579-594 https://doi.org/10.1051/rnd:19980601
  6. Ishimori H, Takahashi Y and Kanagawa H. 1992. Factors affecting survival of mouse blastocysts vitrified by a mixture of ethylene glycol and dimethyl sulfoxide. Theriogenology 38: 1175-1185 https://doi.org/10.1016/0093-691X(92)90129-F
  7. Kasai M, Ito K and Edashige K. 2002. Morphological appearance of the cryopreserved mouse blastocysts as a tool to identy the type of cryoinjury. Hum. Reprod. 17:1863-1874 https://doi.org/10.1093/humrep/17.7.1863
  8. Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T and Machida T. 1990. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fertil. 89:91-97. https://doi.org/10.1530/jrf.0.0890091
  9. King KK, Seide Ge Jr and Elsden RP. 1985. Bovine embryo transfer pregnancies. I Abortion rates and characteristics of calves. J. Anim. Sci. 61:747-757 https://doi.org/10.2527/jas1985.614747x
  10. Kojima T, Tsunoda Y, Ouguri N, Soma T and Sugie T. 1984. Survival of frozen-thawed rabbit morulae in the presence of ethylene glycol. Ipn. J. Anim. Reprod. 30:50-53 https://doi.org/10.1262/jrd1977.30.50
  11. Leibo SP. 1989. Equilibrium and nonequilibrium cryopreservation of embryos. Theriogenology 31:85-93 https://doi.org/10.1016/0093-691X(89)90566-9
  12. Lindl T and Bauer J. 1989. Zell und Gewebekultur. Stuttgart: G. Fischer Verlag
  13. Macfarlane DR, 1986. Devitrification in glass-forming aqueous solutions. Cryobiology 23:230-244 https://doi.org/10.1016/0011-2240(86)90049-0
  14. Miyamoto H and Ishibashi T. 1983. Survival of mouse embryos frozen-thawed slowly or rapidly in the presence of various cryoprotectants. J. Exp. Zool. 226:123-127 https://doi.org/10.1002/jez.1402260114
  15. Nagashima H, Cameron RDA, Kuwayama M, Young M, Beebe L, Blackshaw AW and Nottle MB. 1999. Survival of porcine delipated oocytes and embryos after cryopreservation by freezing or vitrification. J. Reprod. Dev. 45:167-176 https://doi.org/10.1262/jrd.45.167
  16. Pinyopummintr T and Bavister BD. 1991. In vitro-matured/in vitro-fertilized bovine oocytes can develop into morulae/ blastocysts in chemically defined, protein free culture media. Biol. Reprod 45:736-742 https://doi.org/10.1095/biolreprod45.5.736
  17. Pollard JW and Leibo SP. 1993. Comparative cryobiology of in vitro and in vivo produced bovine embryos. Theriogenology 30:287
  18. Rall WF. 1992. Cryopreservation of oocytes and embryos: methods and applications. Anim. Reprod. Sci. 28:237-245 https://doi.org/10.1016/0378-4320(92)90110-Y
  19. Saha S, Otoi T and Suzuki T. 1996. The efficiency of ethylene glycol, trehalose and polyvinylpyrrolidone for successful vitrification of IVF bovine embryos. J. Reprod. Dev. 42: 163-169 https://doi.org/10.1262/jrd.42.163
  20. Saha S, Takagi M, Boediono A and Suzuki T. 1994. Direct rehydration of in vitro fertilised bovine embryos after vitrification. Vet. Rec. 134:276-277 https://doi.org/10.1136/vr.134.11.276
  21. Son DS, Choe CY, Cho SR, Choi SH, Kim HJ, Hur TY, Jung YG, Kang HG and Kim IH. 2007. A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows. J. Reprod. Dev. 53: 1313-1318 https://doi.org/10.1262/jrd.19066
  22. Stringfellow and Sidel SM(Eds). 1998. Manual of the International embryo transfer socity (3rded) International Embryo Transfer Society
  23. Savoy. IL. Voelkel SA and Hu YX. 1992. Use of ethylene glycol as a cryoprotectant for bovine embryos allowing direct transfer of frozen-thawed embryos to recipient female. Theriogenology 37:687-697 https://doi.org/10.1016/0093-691X(92)90148-K
  24. Whitingham DG, Leibo SP and Mazur P. 1972. Survival of mouse embryo frozen to -196${^{\circ}C}$ and -296${^{\circ}C}$. Science 178: 411-414 https://doi.org/10.1126/science.178.4059.411
  25. Yonis AI, Brackett BG and Fayer-Hosken RA. 1989. Influence of serum and hormones on bovine oocyte maturation and fertilization in vitro. Gamete. Res. 23:189-201 https://doi.org/10.1002/mrd.1120230206