Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Fermented Turmeric (Curcuma longa) by Bacillus natto Supplementation on Liver Function and Serum Lipid Parameters in Mice

낫토균으로 발효한 발효울금의 투여가 마우스의 간 기능 및 혈중 지질 함량에 미치는 영향

  • Kang, Jae-Ku (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Kang, Hyo-Jin (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Seo, Ji-Hye (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Kim, Sun-Ok (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Choi, Jung-Hyo (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Cho, Do-Yeun (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Park, Chang-Gyo (Myunggok Medical Research Institute, College of Medicine, Konyang University) ;
  • Lee, Hoi-Young (Myunggok Medical Research Institute, College of Medicine, Konyang University)
  • 강재구 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 강효진 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 서지혜 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 김선옥 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 최정효 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 조도연 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 박창교 (건양대학교 의과대학 명곡의과학연구센타) ;
  • 이회영 (건양대학교 의과대학 명곡의과학연구센타)
  • Published : 2009.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of turmeric and fermented turmeric by Bacillus natto on antioxidant activities, liver function recovery of acute hepatotoxicity mice, and serum lipid parameters in high fat diet fed mice were investigated. In the results of antioxidant activity by DPPH method, fermented turmeric had higher antioxidative activity than turmeric. Acute hepatotoxicity was induced by 0.5 mL of carbon tetrachloride ($CCl_4$) per kg of mice. Unlike turmeric, fermented turmeric significantly reduced the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) after 5 days compared to the controls with 0.5% methyl cellulose (p<0.05). In addition, higher recovery of liver damage by $CCl_4$ was observed in mice with fermented turmeric than with turmeric. High fat (20%) diet fed mice were divided into 4 groups to investigate the effects of turmeric and fermented turmeric on serum lipid parameters: C (vehicle), TuL (low dose (80 mg/kg) with turmeric), TuH (high dose (160 mg/kg) with turmeric), FTuL (low dose with fermented turmeric), and FTuH (high dose with fermented turmeric). The levels of LDL-cholesterol and HDL-cholesterol were significantly reduced and increased in FTuL, FTuH and TuH groups compared to the C group, respectively. However, there was no significant change in triglyceride levels by either turmeric or fermented turmeric compared to those by control. Collectively, these results strongly suggest that fermented turmeric by Bacillus natto could be used as a functional food for enhancement of health with better consumer acceptance.

본 연구는 낫토균으로 울금을 발효함으로써 울금의 기호성 향상 및 낫토키나아제의 작용으로 울금의 체내이용율을 증가시켜 생리활성 기능이 증가하는지 알아보고자 연구하였다. 울금을 낫토균으로 발효시킨 결과, 칼슘과 아연의 경우 울금에 비하여 발효울금에서 각각 약 6배와 44배 증가를 보였고, 셀레늄의 경우에는 발효울금에서만 새롭게 검출되었다. 항산화 활성 비교실험 결과, 울금과 발효울금의 항산화 활성은 각각의 실험농도에서 비슷한 효과를 나타내었으나 발효울금이 울금보다 항산화 활성이 약간 높았다. 발효울금의 간 기능 개선효과에 미치는 영향을 사염화탄소로 간 손상을 일으킨 마우스에서 실험한 결과, AST 및 ALT 효소 활성의 유의적인 감소를 보였고(p<0.05) 특히 사염화탄소에 의해 야기된 간조직의 염증, 괴사 및 지방의 축적이 확연히 적어지는 등 정상군에 가깝게 간 손상이 회복되어 발효울금은 간 기능성 개선에서 울금보다 효과적이었다. 낫토균으로 발효한 발효울금의 혈중 지질 개선효과를 평가하기 위해 고지방(20% 지방) 식이로 유도된 마우스에서 발효울금 및 울금의 경구투여에 의한 혈중 지질 함량 변화를 평가한 결과, 고지방 식이에 의해 증가된 총 콜레스테롤과 LDL 농도를 유의적으로 감소시켰으며 HDL 콜레스테롤 농도는 유의하게 증가시켰다(p<0.05). 본 연구에서 울금 및 발효울금 모두 혈중지질을 효과적으로 개선하였으나, 발효울금의 경우 저농도 투여에 의해서도 혈중 지질 개선효과가 나타나 울금에 비해 상대적인 우수성을 나타내었다. 또한 낫토균에 의한 울금의 발효가 울금 특유의 이취에 의한 쓴맛 및 신맛을 유의하게 개선하는 것으로 평가되어 적절한 제품화 과정을 거친다면 기능뿐만 아니라 소비자의 기호를 향상시킬 수 있는 건강 기능성 식품개발에 기여할 것으로 생각된다.

Keywords

References

  1. Kim CR. 2006. Enhancement of liver function by curcuma extract on acute hepatotoxicity in rat. Korean J Food Sci Ani Resour 26: 386-393
  2. Chi HJ, Kim HS. 1983. Curcumin content of cultivated tumeric in Korea. Korean J Pharmacognosy 14: 67-69
  3. Arora RB, Basu N, Kapoor V, Jain AP. 1971. Anti-inflammatory studies on Curcuma longa (turmeric). Indian J Med Res 59: 1289-1295
  4. Chainani WN. 2003. Safety and anti-inflammatory activity of curcumin: a component of turmeric (Curcuma longa). J Altern Complement Med 9: 161-168 https://doi.org/10.1089/107555303321223035
  5. Aggarwal BB, Kumar A, Bhartic AC. 2003. Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res 23: 363-398
  6. Huang MT, Smart RC, Wong CQ, Conney AH. 1988. Inhibitory effect of curcumin, chlorogenic acid, caffeic acid and ferulic acid on tumor promotion in mouse skin by 12-O-tetradecanoylphorbol-13-acetate. Cancer Res 48: 5941-5946
  7. Ozaki K, Kawata Y, Amano S, Hanazawa S. 2000. Stimulatory effect of curcumin on osteoclast apoptosis. Biochem Pharmacol 52: 437-440
  8. Ferreira LAF, Henriques OB, Andreoni AAS, Vital GRF, Campos MMC, Habermehl GG, Moraes VLG. 1992. Antivenom and biological effects of ar-turmerone isolated from Curcuma longa (Zingiberaceae). Toxicon 30: 1211-1218 https://doi.org/10.1016/0041-0101(92)90437-A
  9. Unnikrishnan MK, Rao MN. 1995. Inhibition of nitrite induced oxidation of hemoglobin by curcuminoids. Pharmazie 50: 490-492
  10. Park EJ, Yoon KR, Kim JH, Kang WS. 1998. Antioxidative property of turmeric (curcumae rhizoma) ethanol extract. Korean J Food Sci Technol 30: 266-271
  11. Deshpande UR, Gadre SG, Raste AS, Pillai D, Bhide SV, Samuel AM. 1998. Protective effect of turmeric (Curcuma longa L) extract on carbon tetrachloride-induced liver damage in rat. Indian J Exp Biol 36: 573-577
  12. Park EJ, Jeon CH, Ko G, Kim J, Sohn DH. 2000. Protective effect of curcumin in rat liver injury induced by carbon tetrachloride. J Pharm Pharmacol 52: 437-440 https://doi.org/10.1211/0022357001774048
  13. Park KJ. 2006. Method for fermenting tumeric and method for making eatable composition having main material for tumeric. Korean Patent 565,989
  14. Kim MO, Oh SC, Kim SS. 2008. Method of manufacturing curcuma domestica fermented extract. Korean Patent 842,022
  15. Milner M, Makise K. 2002. Natto and its active ingredient nattokinase: A potent and safe thrombolytic agent. Alternative & Complementary Therapies 8: 157-164 https://doi.org/10.1089/107628002760091001
  16. Nam HK. 1987. Influence of magnesium and calcium on the serum cholesterol level lowering (III)-Influence of Korea ginseng. J Korean Soc Food Nutr 16: 18-21
  17. Kwon OJ, Choi UK, Lee EJ, Cho YJ, Cha WS, Son DH, Chung YG. 2000. Chemical changes of meju made with barly bran using fermentation. Korean J Food Sci Technol 32: 1135-1141
  18. Ju JC, Shin JH, Lee SJ, Cho HS, Sung NJ. 2006. Antioxidative activity of hot water extracts from medicinal plants. J Korean Soc Food Sci Nutr 35: 7-14 https://doi.org/10.3746/jkfn.2006.35.1.007
  19. Sharma RA, McLelland HR, Hill KA, Ireson CR, Euden SA, Manson MM, Pirmohamed M, Marnett LJ, Gescher AJ, Steward WP. 2001. Pharmacodynamic and pharmacokinetic study of oral curcuma extract in patients with colorectal cancer. Clin Cancer Research 7: 1894-1900
  20. Pan MH, Huang TM, Lin JK. 1999. Biotransformation of curcumin through reduction and glucuronidation in mice. Drug Metab Dispos 27: 486-494
  21. Kim MH, Lee YS, Lee DH, Park HS, Sung CJ. 2001. The study of relation among serum copper, zinc, leptin and lipids of middle-school girls. J Korean Soc Food Sci Nutr 30: 540-546
  22. Jun YS, Sung CJ. 1996. Effects of dietary Fe and Se levels on lipid levels in serum and liver of rats. J Korean Soc Food Sci Nutr 25: 568-574
  23. Kim TH, Son YK, Hwang KH, Kim MH. 2008. Effects of Angelica keiskei Koidzumi and turmeric extract supplementation on serum lipid parameters in hypercholesterolemic diet or P-407-induced hyperlipidemic rats. J Korean Soc Food Sci Nutr 37: 708-713 https://doi.org/10.3746/jkfn.2008.37.6.708

Cited by

  1. Anti-obesity effect of a standardised ethanol extract from Curcuma longa L. fermented with Aspergillus oryzae in ob/ob mice and primary mouse adipocytes vol.92, pp.9, 2012, https://doi.org/10.1002/jsfa.5592
  2. A New Germacrane-Type Sesquiterpene from Fermented Curcuma longa L. vol.35, pp.7, 2014, https://doi.org/10.5012/bkcs.2014.35.7.2201
  3. Improvement Effect of Artificial Rice Containing Curcuma longa L. Extract on Lipid Parameters in C57BL/6J Mice vol.44, pp.8, 2015, https://doi.org/10.3746/jkfn.2015.44.8.1114
  4. Hepatoprotective effects of fermented Curcuma longa L. on carbon tetrachloride-induced oxidative stress in rats vol.151, 2014, https://doi.org/10.1016/j.foodchem.2013.11.058
  5. Effect of Tumeric (Curcuma longa) on Bile Acid and UDP-glucuronyl Transferase Activity in Rats Fed a High-fat and -cholesterol Diet vol.22, pp.8, 2012, https://doi.org/10.5352/JLS.2012.22.8.1064
  6. The effectiveness of fermented turmeric powder in subjects with elevated alanine transaminase levels: a randomised controlled study vol.13, pp.1, 2013, https://doi.org/10.1186/1472-6882-13-58
  7. Antioxidative, Antimicrobial and Anticytotoxic Activities of Seungmagalgeuntang and Fermented Seungmagalgeuntang vol.43, pp.7, 2014, https://doi.org/10.3746/jkfn.2014.43.7.980
  8. Optimized Production of GABA and γ-PGA in a Turmeric and Roasted Soybean Mixture Co-fermented by Bacillus subtilis and Lactobacillus plantarum vol.22, pp.2, 2016, https://doi.org/10.3136/fstr.22.209
  9. Quality Characteristics and Antioxidative Activities of Majakgwa added with Fermented Turmeric Powder vol.29, pp.3, 2013, https://doi.org/10.9724/kfcs.2013.29.3.223
  10. Effects of Fermented Turmeric Extracts on the Obesity in Rats Fed a High-Fat Diet vol.53, pp.1, 2011, https://doi.org/10.5187/JAST.2011.53.1.75
  11. Association Study Between the Polymorphisms of Exostosin-1 Gene and Economic Traits in Hanwoo vol.53, pp.1, 2011, https://doi.org/10.5187/JAST.2011.53.1.7
  12. Hepatoprotective Activity of Fermented Curcuma longa L. on Galactosamine-Intoxicated Rats vol.41, pp.6, 2012, https://doi.org/10.3746/jkfn.2012.41.6.790
  13. Standardized Ethanol Extract ofCurcuma longaL. Fermented byAspergillus oryzaePromotes Lipolysis via Activation of cAMP-Dependent PKA in 3T3-L1 Adipocytes 2013, https://doi.org/10.1111/jfbc.12011
  14. 울금과 강황의 항산화 및 항염증 활성 비교연구 vol.25, pp.1, 2009, https://doi.org/10.6116/kjh.2010.25.1.083
  15. Preclinical Evidence of Curcuma longa and Its Noncurcuminoid Constituents against Hepatobiliary Diseases: A Review vol.2020, pp.None, 2009, https://doi.org/10.1155/2020/8761435