Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Activity of Ethanol Extract from Sargassum thunbergii

지충이(Sargassum thunbergii) 에탄올 추출물의 항균활성

  • Lee, So-Young (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University) ;
  • Song, Eu-Jin (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University) ;
  • Yoon, So-Young (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University) ;
  • Kim, Seo-Jin (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University) ;
  • Lee, So-Jeong (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University) ;
  • Hong, Yong-Ki (Dept. of Biotechnology, Pukyong National University) ;
  • Lim, Sung-Mee (Dept. of Food Science & Technology, Tongmyong University) ;
  • Ahn, Dong-Hyun (Dept. of Food Science & Technology/ Institute of Food Science, Pukyong National University)
  • 이소영 (부경대학교 식품공학과/ 식품연구소) ;
  • 송유진 (부경대학교 식품공학과/ 식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/ 식품연구소) ;
  • 윤소영 (부경대학교 식품공학과/ 식품연구소) ;
  • 김서진 (부경대학교 식품공학과/ 식품연구소) ;
  • 이소정 (부경대학교 식품공학과/ 식품연구소) ;
  • 홍용기 (부경대학교 생물공학과) ;
  • 임성미 (동명대학교 식품공학과) ;
  • 안동현 (부경대학교 식품공학과/ 식품연구소)
  • Published : 2009.04.30
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Abstract

Antimicrobial activity of Sargassum thunbergii was determined by paper disc assay and minimum concentration inhibitor (MIC) test. A water extract of S. thunbergii did not show the antimicrobial activity, but an ethanol extract of S. thunbergii (SHE) inhibited Serratia liquefaciens, Salmonella Typhimurium, Pseudomonas aerogenosa and all of the tested gram-positive bacteria at 4 mg/mL. Especially, Bacillus subtilis, Clostridium perfringens and Listeria monocytogenes were susceptible to SHE. As the results of MIC test, SHE inhibited the growth of B. subtilis, Staphylococcus aureus and Listeria monocytogenes at concentration of $0.1{\sim}0.3%$, and inhibited C. perfringens at 0.01%. In the thermal and pH stability test for SHE, antibacterial activities of SHE were maintained when the SHE was treated at $121^{\circ}C$ for 15 minutes or under pH $2{\sim}8$. SHE was partitioned in the order of n-hexane, chloroform, ethyl acetate and butanol. As the results of the MIC test for each obtained fraction, no fraction exhibited higher antibacterial activity than that of the crude SHE. However, a mixture of chloroform, ethylacetate and ethanol fractions showed higher antibacterial activity than SHE.

Paper disc법을 통하여 항균력을 측정한 결과, 물 추출물에서는 항균효과가 나타나지 않았으나, 4 mg/mL 농도의 에탄올 추출물에서는 그람 음성균 중 S. liquefaciens, S. Typhimurium 및 P. aerogenosa에 대해 항균효과를 보였으며, 실험에 사용된 모든 그람 양성균에 대해 항균효과를 보였다. 특히, B. subtilis, C. perfringens 및 L. monocytogens에 대해 높은 항균력을 보였다. 또한 A. niger 및 P. expansum에는 항진균 효과가 없었으나 S. cerevisae에 대해서는 4 mg/mL 농도에서 항진균 활성을 보였다. 지충이 에탄올 조추출물에 대한 MIC test를 실시한 결과, 그람 음성균에 대한 MIC 값은 $0.6{\sim}0.8%$로 약한 항균활성을 보였으나, 그람 양성 균주 중 C. perfringens와 L. monocytogenes에 대해서는 0.01 및 0.1%에서 두 균주의 생육을 효과적으로 억제하였다. 열 및 pH 안정성 실험 결과, 지충이 에탄올 추출물은 $121^{\cir}C$에서 15분간의 열처리와 pH $2{\sim}8$ 처리에도 항균활성에 변화가 없어 이들 추출물 유래의 항균물질은 열 및 pH 변화에 안정한 물질임을 알 수 있었다. 지충이 에탄올 추출물의 silica gel column chromatography 분획물을 2개 또는 3개씩 1:1(또는 1:1:1)의 비율로 혼합한 후 항균력을 측정한 결과, 클로로포름 및 에탄올 분획 혼합물과 클로로포름, 에틸아세테이트 및 에탄올 분획 혼합물에서 조 추출물 이상의 항균활성이 나타났다.

Keywords

References

  1. Korea food and drug administration. 2005. Statistics of outbreak of food poisoning. Ministry of health and welfare
  2. Cherry JP. 1999. Improving the safety of fresh produce with antimicrobials. Food Technol 53: 54-59
  3. Chang DS, Shin DH, Chung DH, Kim CM, Lee IS. 2002. Food hygiene. Jungmoongak, Seoul. p 244-246
  4. Ferrand C, Marc F, Fritsch P. 2000. Chemical and toxicological studies of products resulting from sorbic acid and methylamine interaction in food conditions. Amino Acids 18: 251-263 https://doi.org/10.1007/s007260050022
  5. Buchanan RL, Shepherd AJ. 1981. Inhibition of Aspergillus parasiticus by thymol. J Food Sci 46: 976-977 https://doi.org/10.1111/j.1365-2621.1981.tb15404.x
  6. Yin MC, Cheng WS. 1998. Inhibition of Aspergillus niger and Aspergillus flavus by some herbs and spices. J Food Prot 61: 123-125 https://doi.org/10.4315/0362-028X-61.1.123
  7. Montes-Belmont R, Carvajal M. 1998. Control of Aspergillus flavus in maize with plant essential oil and their components. J Food Prot 61: 616-619 https://doi.org/10.4315/0362-028X-61.5.616
  8. Ouattara B, Simard RE, Holley RA, Piette GJP, Begin A. 1997. Antibacterial activity of selected fatty acids and essential oil against six meat spoilage organisms. Int J Food Microbiol 37: 155-162 https://doi.org/10.1016/S0168-1605(97)00070-6
  9. Hwang JS, Han YS. 2003. Isolation and identification of antimicrobial compound from Mokdan bark (Paeonia suffruticosa ANDR). J Korean Soc Food Sci Nutr 32: 1059-1065 https://doi.org/10.3746/jkfn.2003.32.7.1059
  10. Choi HY, Han YS. 2003. Isolation and Identification of antimicrobial compound from dansam (Salivia miltiorrhiza Bunge). J Korean Soc Food Sci Nutr 32: 22-28 https://doi.org/10.3746/jkfn.2003.32.1.022
  11. Sim CJ, Lee GH, Jung JH, Yi SD, Kim YH, Oh MJ. 2004. Isolation and identification of antimicrobial activity substances from Rhodiola sachlinensis. Kor J Food Preserv 11: 63-70
  12. Park UY, Kim SH, Kim JH, Kim YG, Chang DS. 1995. Purification of antimicrobial substance for the extract from the root bark of Morus alba. J Fd Hyg Safety 10: 225-230
  13. Awad NE, Selim MA, Saleh MM, Matloub AA. 2003. Seasonal variation of the lipoidal matters and hypolipidaemic activity of the red alga Corallina officinalis L. Phytother Res 17: 19-25 https://doi.org/10.1002/ptr.1005
  14. Ryu BH, Kim DS, Cho K, Sin DB. 1989. Antitumor activity of seaweeds agarne Sarcoma-180. Kor J Food Sci Technol 21: 595-600
  15. Liu JN, Yoshida Y, Wang MQ, Okai Y, Yamachita UB. 1997. B cell stimulating activity of seaweed extracts. Int J Immunopharmac 19: 135-142 https://doi.org/10.1016/S0192-0561(97)00016-7
  16. Iwashima M, Mori J, Ting X, Matsunaga T, Hayashi K, Shinoda D, Saito H, Sankawa U, Hayashi T. 2005. Antioxidant and antiviral activities of plastoquinones from the brown alga Sargassum micracanthum, and a new chromene derivative converted from the plastoquinones. Biol Pharm Bull 28: 374-377 https://doi.org/10.1248/bpb.28.374
  17. Kim JY, Kim KH, Suh HS, Choi WC. 1997. Antiinflammatory effects of new chemical compounds, HS-1580 series (HS-1580, HS-1581, HS-1582). J Life Sci 16: 1181-1187
  18. Yang F, Wang Lin, Hu Qiuhui. 2005. Preparation of polysaccharide derived from Sargassum thunbergii and its antioxidant activity. Food Sci 26: 224-227
  19. Lee HJ, Kim JH, Lee CH, Kim JS, Kwak ST, Lee KB, Song KS, Choi BW, Lee BH. 1999. Inhibitory activities of sea weeds on prolyl endopeptidase, tyrosinase and coagulation. Kor J Pharmacogn 30: 231-237
  20. Kuda T, Kunii T, Goto H, Suzuki T, Yano T. 2007. Varieties of antioxidant and antibacterial properties of Ecklonia kurome products harvested and processed in the Noto Peninsula, Japan. Food Chem 103: 900-905 https://doi.org/10.1016/j.foodchem.2006.09.042
  21. Choi JS, Park HJ, Jung HA, Chung HY, Jung JH, Choi WC. 2000. A cyclohexanonyl bromophenol from the red alga Symphyocladia latiuscula. J Nat Prod 63: 1705-1706 https://doi.org/10.1021/np0002278
  22. Amico G, Oriente G, Piatelli M, Tringali C, Fattorusso E, Mango S, Mayol L. 1980. Diterpenes based on the dolabellane skeleton from Dictyota dichotoma. Tetrahedron 36: 1409-1414 https://doi.org/10.1016/0040-4020(80)85055-1
  23. Glombitza KW, Rosener HU, Vilter H, Rauwald W. 1973. Antibiotics from algae. 8. Phloroglucinol from Phaeophyceae. Planta Med 24: 301-303 https://doi.org/10.1055/s-0028-1099502
  24. Fenical W, Sims JJ. 1973. Zonarol and isozonarol, fungitoxic hydroguinones from the brown seaweed Dictyopteris zonarioides. J Org Chem 38: 2383-2386 https://doi.org/10.1021/jo00953a022
  25. Lim CW, Lee JS, Cho YJ. 2000. Structure and some properties of the antimicrobial compounds in the red alga, Symphyosladia latiscula. J Korean Fish Soc 33: 280-287
  26. Kim SH, Lim SB, Ko YH, Oh MC, Park CS. 1994. Extraction yields of Hizikia fusiforme by solvents and their antimicrobial effects. Bull Korean Fish Soc 27: 462-468
  27. Kim JH, Lee DS, Lim CW, Park HY, Park JH. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. J Korean Fish Soc 35: 191-195 https://doi.org/10.5657/kfas.2002.35.2.191
  28. Seo Y, Lee HJ, Park KE, Kim YA, Ahn JW, Yoo JS, Lee BJ. 2004. Peroxynitrite-scavenging constituents from the brown alga Sargassum thunbergii. Biosci Biotech Eng 9: 212-216 https://doi.org/10.1007/BF02942295
  29. Zhuang C, Itoh H, Mizuno T. 1995. Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii). Biosci Biotech Biochem 59: 563-567 https://doi.org/10.1271/bbb.59.563
  30. Kang SY, Oh MJ, Shin JA. 2005. Antimicrobial activity of korean marine algae against fish pathogenic bacteria. J Fish Pathol 18: 147-156
  31. Lee JH, Lee KH, Yoo HI, Zhou XL, Kim YS, Han GC, Nam KW. 2006. Antimicrobial activity of Neorhodomela aculeata extracts against human skin pathogens. J Kor Fish Soc 39: 292-296 https://doi.org/10.5657/kfas.2006.39.3.292
  32. Val AG, Platas G, Basilio A, Cabello A, Gorrochategui J, Suay I, Vicente F, Portillo M, Rio MJ, Reina GG, Pelaez F. 2001. Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary islands, Spain). Int Microbiol 4: 35-40
  33. Murakami M, Makabe K, Okada S, Yamaguchi K, Konosu S. 1988. Screening of biologically activity compounds in microalgae. Nippon Suisan Cakki Shi 54: 1035-1039 https://doi.org/10.2331/suisan.54.1035
  34. Nadal NGM, Casillias CM, Rodriguez LV, Rodriguez JR, Vera LT. 1966. Antibiotic properties of marine algae-III. Cymopolia barbara. Bot Marina 9: 121-126
  35. Kubo I, Himejima M, Tsujimoto K, Muroi H, Ichikawa N. 1992. Antibacterial activity of crinitol and its potentiation. J Nat Prod 55: 780-785 https://doi.org/10.1021/np50084a012
  36. EI-Shenawy MA, Marth EH. 1989. Behavior of Listeria monosytogenes in the presence of sodium propionate. J Food Microbiol 8: 85-92 https://doi.org/10.1016/0168-1605(89)90084-6
  37. Park CS, Cha MS. 2000. Comparison of antibacterial activity of green tea extracts and preservation to the pathogenic bacteria. Korean J Food Nutr 13: 36-44
  38. Morales JL, Cantillo-Ciau ZO, Sanchez-Molina I, Mena-Rejon GJ. 2006. Screening of antibacterial and antifungal activity of six marine macroalgae from coasts of Yucatan Peninsula. Pharm Biol 44: 632-635 https://doi.org/10.1080/13880200600897569
  39. Magallanes C, Cordoba C, Orozco R. 2003. Antimicrobial activity of ethanolic extracts of marine algae from central coast of Peru. Rev Peru Biol 10: 125-132
  40. Nakamura S, Kato AM, Kobayashi K. 1991. New antimicrobial characteristics of lysozyme-dextran conjugate. J Agric Food Chem 39: 647-650 https://doi.org/10.1021/jf00004a003
  41. Kim JH, Lee DS, Lim CW, Park HY, Park JH. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. J Korean Fish Soc 35: 191-195 https://doi.org/10.5657/kfas.2002.35.2.191
  42. Yoon JW, Yoo MY, Choi JH, Lee MK, Oh DH. 2005. Antimicrobial effects of ethanol extracted and sub-fractionated materials from different parts of Quercus aliena Blume. J Korean Soc Food Sci Nutr 34: 910-914 https://doi.org/10.3746/jkfn.2005.34.6.910
  43. Kong MY, Kang TS, Lee MK, Park BK, Oh DH. 2001. Antimicrobial and antioxidative activities of solvent fractions of Quercus mongolica leaf. J Korean Soc Food Sci Nutr 30: 415-420
  44. Seo YW, Park KE, Nam TJ. 2007. Isolation of a new chromene from the brown alga Sargassum thunbergii. Bull Korean Chem Soc 28: 1831-1833 https://doi.org/10.5012/bkcs.2007.28.10.1831
  45. Kim YH, Kim EH, Lee CH, Kim MH, Rho JR. 2007. Two new monogalactosyl diacylglycerols from brown alga Sargassum thunbergii. Lipids 42: 395-399 https://doi.org/10.1007/s11745-007-3035-7
  46. Chimenti F, Bizzarri B, Bolasco A, Secci D, Chimenti P, Carradori S, Granese A, Rivanera D, Lilli D, Zicari A, Scaltrito MM, Sisto F. 2007. A novel class of selective anti-Helicobacter pylori agents 2-one-2H-chromene-3-carboxamide derivatives. Bioorg Med Chem Lett 17: 3065-3071 https://doi.org/10.1016/j.bmcl.2007.03.050

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