Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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The Protective Effect of Ginseng Saponin against High Glucose-Induced Secretion of Insulin-Like Growth Factor (IGF)-I in Primary Cultured Rabbit Proximal Tubule Cells

신장 근위세뇨관 세포에서 고포도당에 의한 IGF-I 분비 촉진작용에 있어서 인삼의 차단효과

  • Jung, Ho-Kyoung (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Lim, Suel-Ki (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Min-Jung (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Chun-Sik (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Yoon, Kyung-Chul (Department of Ophthalmology, Chonnam National University Medical School and Hospital) ;
  • Han, Ho-Jae (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Soo-Hyun (Bio-therapy Human Resources Center, animal metical institute Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
  • 정호경 (전남대학교 수의과대학 바이오치료 산업인력 양성팀, 동물의학연구소) ;
  • 임슬기 (전남대학교 수의과대학 바이오치료 산업인력 양성팀, 동물의학연구소) ;
  • 박민정 (전남대학교 수의과대학 바이오치료 산업인력 양성팀, 동물의학연구소) ;
  • 배춘식 (전남대학교 수의과대학 바이오치료 산업인력 양성팀, 동물의학연구소) ;
  • 윤경철 (전남대학교 의과대학 안과학교실) ;
  • 한호재 (전남대학교 수의과대학 바이오치료 산업인력 양성팀, 동물의학연구소) ;
  • 박수현 (전남대학교 수의과대학 바이오치료 산업인력 양성팀, 동물의학연구소)
  • Published : 2009.03.31
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Abstract

Diabetic nephropathy is associated with the dysfunction of proximal tubule cells. Insulin-like growth factor 1(IGF-I) has also been considered to play an important role in the development of diabetic nephropathy. Ginsenosides have been used as a remedy for diabetes in Asian countries. Therefore, we examined the preventive effect of ginsenosides against high glucose-induced alteration of IGF-I secretion in the primary cultured proximal tubule cells. In present study, Ginseng saponin (GS) completely blocked high glucose-induced stimulation of IGF-I secretion in proximal tubule cells, whereas panaxatriol (PI) and panaxadiol (PD) partially suppressed. In addition, high glucose stimulated cAMP formation and protein kinase C(PKC) activity from cytosolic to membrane fraction. GS completely prevented high glucose-induced stimulation of cAMP and PKC activity while PT and PD partially did. Furthermore, high glucose-induced stimulation of IGF-I was blocked by the treatment of PKI (protein kinase A inhibitor) and bisindolylmaleimide I (protein kinase C inhibitor). In conclusion, GS prevented high glucose-induced dysfunction of proximal tubule cells.

인삼은 전통적으로 항당뇨 효과가 있는 것으로 보고되고 있다. Insulin-like growth factor (IGF)-I 역시 당뇨병성 신증의 발병 초기에 중요한 역할을 하는 것으로 알려져 있다. 이에 본 연구에서는 신장 근위세뇨관 세포에서 고포도당에 의한 IGF-I 분비에 대한 ginsenoside의 차단 효과 및 이와 관련된 신호전달계를 알아보았다. 결과는 다음과 같다. 고포도당에 의해 증가되었던 IGF-I분비 촉진 작용은 GTS, PD 및 PT 처리 시 차단되었으며, 세포 성장 작용 (세포 비대)에서도 같은 효과를 볼 수 있었다. 아울러 고포도당에 의한 cAMP 및 PKC 활성은 GTS 처리시 현저하게 차단되었으며 PD 및 PT 처리 시 역시 부분적으로 억제되는 것으로 나타났다. 이상의 결과를 볼 때 신장 근위세뇨관 세포에서 ginsenoside는 cAMP 및 PKC 활성 경로를 억제하여 고포도당에 의한 IGFs 분비 작용을 차단하는 것으로 나타났다.

Keywords

References

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