Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Rapid detection and quantification of porcine circovirus type 2 (PCV 2) DNA in Real-time PCR

Real-time PCR을 이용한 돼지써코바이러스 감염증 진단법 연구

  • 김은경 (경상남도축산진흥연구소) ;
  • 황보원 (경상남도축산진흥연구소) ;
  • 이종민 (경상남도축산진흥연구소) ;
  • 손병국 (경상남도축산진흥연구소) ;
  • 박호정 (경상남도축산진흥연구소) ;
  • 김도경 (경상남도축산진흥연구소)
  • Published : 2009.12.30
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Abstract

Assay for the detection and quantification of porcine circovirus type 2 (PCV 2) with the real-time PCR were developed. TaqMan probe real-time using a set of primer/probe was developed for detection of PCV 2. In this study we applied real-time PCR assay to 320 samples, collected from pig farms. In 151 of 320 samples, PCV 2 DNA was detected by conventional PCR assay. All samples positive for PCV 2 DNA in conventional PCR assay were also positive in Real-time PCR assay, but 69 of 169 samples that tested negative for PCV 2 DNA in conventional assay were tested positive in TaqMan probe real-time PCR assay. The test of TaqMan probe real-time PCR resulted in detection and quantification limits of 101 copies per sample. TaqMan probe real-time PCR assay increased the number of samples in which PCV 2 was detected by 21%. TaqMan probe real-time PCR assay is very efficient method in contrast to the conventinal PCR, becoming increasingly important method for gene analysis.

Keywords

References

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