Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Four Different Isolation Media for Staphylococcus aureus

황색포도상구균 분리배지 비교

  • Oh, Min-Hee (Food Safety Research Division, Korea Food Research Institute) ;
  • Kang, Seong-Il (3M Korea Innovation Center) ;
  • Hong, Sang-Phil (Food Safety Research Division, Korea Food Research Institute) ;
  • Oh, Se-Wook (Food Safety Research Division, Korea Food Research Institute)
  • Published : 2009.05.29
Download PDF
⟨ Previous Next ⟩

Abstract

Performance test was carried out between selective media which are generally used in Staphylococcus aureus isolation from food. Sensitivity, determined according to the appearance of characteristic colonies when 30 different S. aureus strains were tested, resulted as Baird-Parker agar (RPF)> $Petrifilm^{TM}$ Staph Express plate> Baird-Parker agar> Mannitol salt agar. Also, the four different media showed the same selectivity because all tested media did not produce the false positive colonies. Recovery efficiency from the artificially inoculated foodstuff was almost the same for the tested media. Presumptive colonies were collected from the dried fishery product using Mannitol salt agar and collected strains were tested on 4 different selective agar. Almost presumptive strains did not show the false positive colonies except for S. carnosus ssp carnosus. This strain was identified as false positive colonies on Mannitol salt agar, Baird-Parker agar and $Petrifilm^{TM}$ Staph Express plate. But Baird-Parker agar (RPF) did not show the false positive colonies with the same strains. So, it was concluded that the Baird-Parker agar (RPF) has more higher selectivity than other tested media in this experiment.

황색포도상구균 분리에 사용되고 있는 난황첨가 만니톨 식염한천배지, Baird-Parker 한천평판배지, Baird-Parker 한천평판배지(RPF) 및 Petrifilm에 대하여 성능 비교실험을 실시하였다. 민감도는 30종의 황색포도상구균에 대하여 분리배지의 특징적인 콜로니를 형성하는 비율로 측정하였는데 Baird-Parker 한천평판배지(RPF)> Petrifilm> Baird-Parker 한천평판배지> 난황첨가 만니톨 식염한천배지의 순으로 나타났다. 황색포도상구균이 아닌 균주를 이용하여 측정한 선택성은 4종 분리배지 모두 위양성을 나타내지 않았다. 인위적으로 접종한 식품에서의 회수실험은 4종 배지 모두 회수 효율이 비슷한 것으로 측정되었다. 수산건제품에서 황색포도상구균으로 추정되는 콜로니를 분리하여 4종 배지에서 위양성, 위음성 발현여부를 측정하였다. 그 결과, 황색포도상구균 이외의 대부분 분리균주에서는 위양성이 나타나지 않았지만 S. carnosus ssp carnosus의 경우 난황첨가 만니톨 식염한천배지, Baird-Parker 한천평판배지와 Petrifilm에서 위양성을 나타내었다. 그러나 Baird-Parker 한천평판배지(RPF)에서는 위양성 반응이 나타나지 않아 실험에 공시된 배지 중에서는 선택성이 상대적으로 높은 것으로 판단되었다.

Keywords

References

  1. KFDA, 2009. Available from: http://e-stat.kfda.go.kr. Accessed Jan. 12
  2. 식품의약품안전청 고시. 2008. 제 2008-15호. 식품의 기준 및 규격 개정 고시
  3. Bergodoll MS, Huang IY, Schantz EJ. 1974. Chemistry of the staphylococcal enterotoxins. J Agric Food Chem 22: 9-13 https://doi.org/10.1021/jf60191a001
  4. Rosec JP, Guiraud JP, Dalet C, Richard N. 1997. Enterotoxin production by staphylococci isolated from foods in France. Int J Food Microbiol 37: 213-221 https://doi.org/10.1016/S0168-1605(96)01234-2
  5. Kim ES, Jhon DY. 1996. Rapid detection of enterotoxigenic Staphylococcus aureus by polymerase chain reaction. Korean J Food Sci Technol 28: 1001-1008
  6. Park SY, Choi JW, Yeon JH, Lee MJ, Lee DH, Kim KS, Park KH, Ha SD. 2005. Assessment of contamination levels of foodborne pathogens isolated in major RTE foods marketed in convenience stores. Korean J Food Sci Technol 37: 274-278
  7. Yoon SK, Kang YS, Sohn MG, Kim CM, Park J. 2007. Prevalence of enterotoxigenic Staphylococcus aureus in retail ready-to-eat Korean Kimbab rolls. Food Sci Biotechnol 16: 621-625
  8. Bahk GJ, Oh DH, Ha SD, Park KH, Joung MS, Chun SJ, Park JS, Woo GJ, Hong CH. 2005. Quantitative microbial risk assessment model for Staphylococcus aureus in Kimbab. Korean J Food Sci Technol 37: 484-491
  9. Jinsung Unitech. 2007. Food-borne pathogens (Handbook)
  10. Baird-Parker AC. 1962. An improved diagnostic and selective medium for isolating coagulase-positive staphylococci. J Appl Bacteriol 25: 12-19 https://doi.org/10.1111/j.1365-2672.1962.tb01113.x
  11. Maxine EM, Eleanor BT. 1964. Comparison of several selective media for isolation and differentiation of coagulase-positive strains of Staphylococcus aureus. Appl Microbiol 12: 169-172
  12. Wendy AM, Victoria AA, Ann MS. 2003. $3M^{TM} Petrifilm^{TM}$ Staph Express count plate method for the enumeration of Staphylococcus aureus in selected types of meat, seafood, and poultry: Collaborative study. J AOAC Int 86: 947-953
  13. Niskanen A, Aalto M. 1978. Comparison of selective media for coagulase-positive enterotoxigenic Staphylococcus aureus. Appl Environ Microbiol 35: 1233-1236
  14. Philppe M, Jose ME, Patrick F, Damien M, Timotiiy JF, Patrice F, Pierre V. 1995. Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of expermental endocarditis. Infect and Immun 63: 4738-4743
  15. Schleifer KH, Fischer U. 1982. Description of a new species of the genus Staphylococcus: Staphylococcus carnosus. Int J Syst Bacteriol 32: 153-156 https://doi.org/10.1099/00207713-32-2-153
  16. Probst AJ, Hertel C, Richer L, Wassill L, Ludwig W, Hammes W. 1998. Staphylococcus condimenti sp. nov., from soy sauce mash, and Staphylococcus carnosus (Schleifer and Fischer 1982) subsp. utilis subsp. nov. Int J Syst Bacteriol 48: 651-658 https://doi.org/10.1099/00207713-48-3-651

Cited by

  1. Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products vol.27, pp.2, 2012, https://doi.org/10.13103/JFHS.2012.27.2.169
  2. 축·수산·전통식품 중 황색포도상 구균의 정량적 분석을 위한 PetrifilmTM Staph Express Count Plate의 성능 평가 vol.34, pp.3, 2009, https://doi.org/10.13103/jfhs.2019.34.3.309